The origin of anti-nuclear antibodies in bcl-2 transgenic mice

Laura Mandik-Nayak, Sudhir Nayak, Caroline Sokol, Ashlyn Eaton-Bassiri, Michael P. Madaio, Andrew J. Caton, Jan Erikson

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

bcl-2 transgenic mice develop anti-double-stranded (ds) DNA antibodies similar to those present in systemic lupus erythematosus. To begin to understand where a breakdown in the regulation of autoreactive lymphocytes is occurring, we have used a bcl-2 transgene (Tg) in conjunction with an Ig Tg that allows us to identify and track anti-dsDNA B cells. Previously, we have shown that anti-dsDNA a cells are actively tolerized in BALB/c mice as manifested by their developmental arrest, follicular exclusion, increased in vivo turnover rate and lack of their antibody in the serum. The bcl-2 Tg mice increased the lifespan of anti-dsDNA a cells, but did not alter the other features of tolerance, indicating that the anergy of the anti-dsDNA a cells is independent of their reduced lifespan. Furthermore, these data suggest that the serum anti-dsDNA antibodies in bcl-2 transgenic mice are not due to a breakdown in the induction or maintenance of a cell anergy; rather they may originate from a cells that have transited through a germinal center.

Original languageEnglish (US)
Pages (from-to)353-364
Number of pages12
JournalInternational Immunology
Volume12
Issue number3
DOIs
StatePublished - Jan 1 2000

Fingerprint

Transgenic Mice
Anti-Idiotypic Antibodies
Transgenes
Germinal Center
Antibodies
Serum
Systemic Lupus Erythematosus
B-Lymphocytes
Maintenance
Lymphocytes
DNA

Keywords

  • Anti-nuclear antibodies
  • Apoptosis
  • Autoimmunity
  • Germinal center
  • Tolerance

ASJC Scopus subject areas

  • Immunology

Cite this

Mandik-Nayak, L., Nayak, S., Sokol, C., Eaton-Bassiri, A., Madaio, M. P., Caton, A. J., & Erikson, J. (2000). The origin of anti-nuclear antibodies in bcl-2 transgenic mice. International Immunology, 12(3), 353-364. https://doi.org/10.1093/intimm/12.3.353

The origin of anti-nuclear antibodies in bcl-2 transgenic mice. / Mandik-Nayak, Laura; Nayak, Sudhir; Sokol, Caroline; Eaton-Bassiri, Ashlyn; Madaio, Michael P.; Caton, Andrew J.; Erikson, Jan.

In: International Immunology, Vol. 12, No. 3, 01.01.2000, p. 353-364.

Research output: Contribution to journalArticle

Mandik-Nayak, L, Nayak, S, Sokol, C, Eaton-Bassiri, A, Madaio, MP, Caton, AJ & Erikson, J 2000, 'The origin of anti-nuclear antibodies in bcl-2 transgenic mice', International Immunology, vol. 12, no. 3, pp. 353-364. https://doi.org/10.1093/intimm/12.3.353
Mandik-Nayak L, Nayak S, Sokol C, Eaton-Bassiri A, Madaio MP, Caton AJ et al. The origin of anti-nuclear antibodies in bcl-2 transgenic mice. International Immunology. 2000 Jan 1;12(3):353-364. https://doi.org/10.1093/intimm/12.3.353
Mandik-Nayak, Laura ; Nayak, Sudhir ; Sokol, Caroline ; Eaton-Bassiri, Ashlyn ; Madaio, Michael P. ; Caton, Andrew J. ; Erikson, Jan. / The origin of anti-nuclear antibodies in bcl-2 transgenic mice. In: International Immunology. 2000 ; Vol. 12, No. 3. pp. 353-364.
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