The proteasome-associated deubiquitinating enzyme Usp14 is essential for the maintenance of synaptic ubiquitin levels and the development of neuromuscular junctions

Ping Chung Chen, Lu Ning Qin, Xiao Ming Li, Brandon J. Walters, Julie A. Wilson, Lin Mei, Scott M. Wilson

Research output: Contribution to journalArticle

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Abstract

Dysfunction of the ubiquitin proteasome system (UPS) has been implicated in the pathogenesis of many neurological diseases, including Alzheimer's, spinocerebellar ataxia, and several motor neuron diseases. Recent research indicates that changes in synaptic transmission mayplay a critical role in the progression of neurological disease; however, the mechanisms by which the UPS regulates synaptic structure and function have not been well characterized. In this report, we show that Usp14 is indispensable for synaptic development and function at neuromuscular junctions (NMJs). Usp14-deficient axJ mice display a resting tremor, a reduction in muscle mass, and notable hindlimb rigidity without any detectable loss of motor neurons. Instead, loss of Usp14 causes developmental defects at motor neuron endplates. Presynaptic defects include phosphorylated neurofilament accumulations, nerve terminal sprouting, and poor arborization of the motor nerve terminals, whereas postsynaptic acetylcholine receptors display immature plaque-like morphology. These structural changes in the NMJ correlated with ubiquitin loss in the spinal cord and sciatic nerve. Further studies demonstrated that the greatest loss of ubiquitin was found in synaptosomal fractions, suggesting that the endplate swellings may be caused by decreased protein turnover at the synapse. Transgenic restoration of Usp14 in the nervous system corrected the levels of monomeric ubiquitin in the motor neuron circuit and the defects that were observed in the motor endplates and muscles of the axJ mice. These data define a critical role for Usp14 at mammalian synapses and suggest a requirement for local ubiquitin recycling by the proteasome to control the development and function of NMJs.

Original languageEnglish (US)
Pages (from-to)10909-10919
Number of pages11
JournalJournal of Neuroscience
Volume29
Issue number35
DOIs
StatePublished - Sep 2 2009

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Neuromuscular Junction
Proteasome Endopeptidase Complex
Ubiquitin
Maintenance
Motor Neurons
Motor Endplate
Synapses
Spinocerebellar Ataxias
Muscles
Spinal Nerves
Motor Neuron Disease
Intermediate Filaments
Recycling
Cholinergic Receptors
Tremor
Sciatic Nerve
Hindlimb
Synaptic Transmission
Nervous System
Disease Progression

ASJC Scopus subject areas

  • Neuroscience(all)

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The proteasome-associated deubiquitinating enzyme Usp14 is essential for the maintenance of synaptic ubiquitin levels and the development of neuromuscular junctions. / Chen, Ping Chung; Qin, Lu Ning; Li, Xiao Ming; Walters, Brandon J.; Wilson, Julie A.; Mei, Lin; Wilson, Scott M.

In: Journal of Neuroscience, Vol. 29, No. 35, 02.09.2009, p. 10909-10919.

Research output: Contribution to journalArticle

Chen, Ping Chung ; Qin, Lu Ning ; Li, Xiao Ming ; Walters, Brandon J. ; Wilson, Julie A. ; Mei, Lin ; Wilson, Scott M. / The proteasome-associated deubiquitinating enzyme Usp14 is essential for the maintenance of synaptic ubiquitin levels and the development of neuromuscular junctions. In: Journal of Neuroscience. 2009 ; Vol. 29, No. 35. pp. 10909-10919.
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