The regulation of inflammatory cytokine secretion in macrophage cell line by the chemical constituents of Rhus sylvestris

Yan Ding, Huu Tung Nguyen, Sung In Kim, Ha Won Kim, Young Ho Kim

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

In our preliminary screening study on the anti-inflammatory activity, eight triterpenes, one sterol, and one chalcone were isolated from the CH2Cl2-soluble extract of the stems and leaves of Rhus sylvestris Siebold and Zucc (Anacardiaceae). On the basis of their spectroscopic data, these compounds were identified as 10α-cucurbitadienol (1), glut-5-en-3-ol (2), β-amyrin acetate (3), β-amyrin (4) and lupeol (5), cycloart-24-en-3-one (6), cycloart-25-en-3,24-dione (7), 24-hydroxycycloart-25-en-3-one (8), β-sitosterol (9), and 2′-hydroxy-4,4′-dimethoxychalcone (10). All of them were isolated from this plant for the first. Furthermore, the compounds in non-cytotoxic concentrations (0-1.0 μM) were tested for their ability to block inflammatory cytokine secretion in the presence of LPS in the murine RAW264.7 macrophage cell line. Among the compounds that were tested, compounds 8 and 9 reduced the LPS-induced secretion of IL-6, as well as TNF-α, in a mouse RAW264.7 macrophage cell line. Moreover, compounds 2, 3, 7, and 10 specifically diminished only the secretion of TNF-α even in 0.01 μM concentrations. It is thus suggested that they are potential therapeutics of TNF-α-related diseases and conditions, such as transplant rejection, type II diabetes, and atherosclerosis.

Original languageEnglish (US)
Pages (from-to)3607-3610
Number of pages4
JournalBioorganic and Medicinal Chemistry Letters
Volume19
Issue number13
DOIs
StatePublished - Jul 1 2009
Externally publishedYes

Fingerprint

Rhus
Macrophages
Cells
Anacardiaceae
Cytokines
Chalcone
Cell Line
Transplants
Triterpenes
Graft Rejection
Sterols
Medical problems
Type 2 Diabetes Mellitus
Interleukin-6
Atherosclerosis
Screening
Anti-Inflammatory Agents
Therapeutics

Keywords

  • Chalcone
  • Cytotoxic
  • Inflammatory cytokine
  • Interleukin-6
  • Rhus sylvestris
  • Triterpenoids
  • Tumor necrosis factor-α

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

Cite this

The regulation of inflammatory cytokine secretion in macrophage cell line by the chemical constituents of Rhus sylvestris. / Ding, Yan; Nguyen, Huu Tung; Kim, Sung In; Kim, Ha Won; Kim, Young Ho.

In: Bioorganic and Medicinal Chemistry Letters, Vol. 19, No. 13, 01.07.2009, p. 3607-3610.

Research output: Contribution to journalArticle

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