The renaturation and purification of RGD-staphylokinase by gel filtration

A. Cheng, Gang Song, Hua Bo Su, Min Yu, Yu Yang Li, Hou Yan Song

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

A recombinant RGD-Staphylokinase(RGD-Sak) with thrombolytic and anti-thrombolytic bifunction was expressed in E. coli. The expression product accumulates as inclusion bodies. In order to obtain active molecule, the RGD-Sak in the inclusion body should be denatured and then renatured. The renaturation of RGD-Sak was performed by gel filtration. Comparing with the traditional way of dilution renaturation, gel filtration way is better than the traditional one, since there are some advantages, such as simple processing, high recovery, low cost and higher purity after renaturation, After renaturation, RGD-Sak was purified by Q-Sepharose FF, and the purity was more than 95%. Analysis of CD spectra showed that the final product from the two renaturation ways have similar CD spectra. It was demonstrated that RGD-Sak molecules proceeded correct refolding through gel filtration or dilution renaturation process.

Original languageEnglish (US)
Pages (from-to)693-697
Number of pages5
JournalSheng wu gong cheng xue bao = Chinese journal of biotechnology
Volume18
Issue number6
StatePublished - Nov 2002
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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