The role of calcium/calmodulin-activated calcineurin in rapid and slow endocytosis at central synapses

Tao Sun, Xin Sheng Wu, Jianhua Xu, Benjamin D. McNeil, Zhiping P. Pang, Wanjun Yang, Li Bai, Syed Qadri, Jeffery D. Molkentin, David T. Yue, Ling Gang Wu

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Abstract

Although the calcium/calmodulin-activated phosphatase calcineurin may dephosphorylate many endocytic proteins, it is not considered a key molecule in mediating the major forms of endocytosis at synapses - slow, clathrin-dependent and the rapid, clathrin-independent endocytosis. Here we studied the role of calcineurin in endocytosis by reducing calcium influx, inhibiting calmodulin with pharmacological blockers and knockdown of calmodulin, and by inhibiting calcineurin with pharmacological blockers and knock-out of calcineurin. These manipulations significantly inhibited both rapid and slow endocytosis at the large calyx-type synapse in 7- to 10-d-old rats and mice, and slow, clathrin-dependent endocytosis at the conventional cultured hippocampal synapse of rats and mice. These results suggest that calcium influx during nerve firing activates calcium/calmodulin-dependent calcineurin, which controls the speed of both rapid and slow endocytosis at synapses by dephosphorylating endocytic proteins. The calcium/calmodulin/calcineurin signaling pathway may underlie regulation of endocytosis by nerve activity and calcium as reported at many synapses over the last several decades.

Original languageEnglish (US)
Pages (from-to)11838-11847
Number of pages10
JournalJournal of Neuroscience
Volume30
Issue number35
DOIs
StatePublished - Sep 1 2010

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Calcineurin
Calmodulin
Endocytosis
Synapses
Calcium
Clathrin
Pharmacology
Proteins

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

The role of calcium/calmodulin-activated calcineurin in rapid and slow endocytosis at central synapses. / Sun, Tao; Wu, Xin Sheng; Xu, Jianhua; McNeil, Benjamin D.; Pang, Zhiping P.; Yang, Wanjun; Bai, Li; Qadri, Syed; Molkentin, Jeffery D.; Yue, David T.; Wu, Ling Gang.

In: Journal of Neuroscience, Vol. 30, No. 35, 01.09.2010, p. 11838-11847.

Research output: Contribution to journalArticle

Sun, T, Wu, XS, Xu, J, McNeil, BD, Pang, ZP, Yang, W, Bai, L, Qadri, S, Molkentin, JD, Yue, DT & Wu, LG 2010, 'The role of calcium/calmodulin-activated calcineurin in rapid and slow endocytosis at central synapses', Journal of Neuroscience, vol. 30, no. 35, pp. 11838-11847. https://doi.org/10.1523/JNEUROSCI.1481-10.2010
Sun, Tao ; Wu, Xin Sheng ; Xu, Jianhua ; McNeil, Benjamin D. ; Pang, Zhiping P. ; Yang, Wanjun ; Bai, Li ; Qadri, Syed ; Molkentin, Jeffery D. ; Yue, David T. ; Wu, Ling Gang. / The role of calcium/calmodulin-activated calcineurin in rapid and slow endocytosis at central synapses. In: Journal of Neuroscience. 2010 ; Vol. 30, No. 35. pp. 11838-11847.
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