The role of CRF2 receptors in corticotropin-releasing factor- and urocortin-induced anorexia

Gennady N. Smagin, Leigh A. Howell, Donna H. Ryan, Errol B. De Souza, Ruth B.S. Harris

Research output: Contribution to journalArticle

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Abstract

The experiments presented in this study were designed to assess corticotropin-releasing factor (CRF) receptor subtype mediation of CRF- and urocortin (UCN)-induced decrease in food intake. Male Sprague-Dawley rats were treated with antisense and sense oligonucleotides (ON) to CRF2 receptor mRNAs for 36 h and then received an intracerebroventricular (i.c.v.) injection of CRF, UCN (3 μg) or saline. Antisense treatment significantly attenuated CRF- and UCN-induced suppression in food intake and HPA activation. Administration of CRF1 receptor antagonist did not affect the decrease in food intake or activation of the HPA axis induced by ICV infusion of 3 μg CRF. The data suggest that down-regulation of CRF2 receptors selectively attenuates CRF- and UCN-induced anorexia and hypothalamo- pituitary-adrenocortical activation in rats.

Original languageEnglish (US)
Pages (from-to)1601-1606
Number of pages6
JournalNeuroReport
Volume9
Issue number7
DOIs
StatePublished - May 11 1998
Externally publishedYes

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Keywords

  • Antisense oligonucleotides
  • Corticosterone
  • HPA axis
  • Selective antagonist

ASJC Scopus subject areas

  • Neuroscience(all)

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