The role of CRF2 receptors in corticotropin-releasing factor- and urocortin-induced anorexia

Gennady N. Smagin, Leigh A. Howell, Donna H. Ryan, Errol B. De Souza, Ruth Babette Harris

Research output: Contribution to journalArticle

137 Citations (Scopus)

Abstract

The experiments presented in this study were designed to assess corticotropin-releasing factor (CRF) receptor subtype mediation of CRF- and urocortin (UCN)-induced decrease in food intake. Male Sprague-Dawley rats were treated with antisense and sense oligonucleotides (ON) to CRF2 receptor mRNAs for 36 h and then received an intracerebroventricular (i.c.v.) injection of CRF, UCN (3 μg) or saline. Antisense treatment significantly attenuated CRF- and UCN-induced suppression in food intake and HPA activation. Administration of CRF1 receptor antagonist did not affect the decrease in food intake or activation of the HPA axis induced by ICV infusion of 3 μg CRF. The data suggest that down-regulation of CRF2 receptors selectively attenuates CRF- and UCN-induced anorexia and hypothalamo- pituitary-adrenocortical activation in rats.

Original languageEnglish (US)
Pages (from-to)1601-1606
Number of pages6
JournalNeuroReport
Volume9
Issue number7
DOIs
StatePublished - May 11 1998
Externally publishedYes

Fingerprint

Urocortins
Corticotropin-Releasing Hormone
Anorexia
Eating
Corticotropin-Releasing Hormone Receptors
Antisense Oligonucleotides
Sprague Dawley Rats
Down-Regulation
CRF receptor type 2
Messenger RNA
Injections

Keywords

  • Antisense oligonucleotides
  • Corticosterone
  • HPA axis
  • Selective antagonist

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

The role of CRF2 receptors in corticotropin-releasing factor- and urocortin-induced anorexia. / Smagin, Gennady N.; Howell, Leigh A.; Ryan, Donna H.; De Souza, Errol B.; Harris, Ruth Babette.

In: NeuroReport, Vol. 9, No. 7, 11.05.1998, p. 1601-1606.

Research output: Contribution to journalArticle

Smagin, Gennady N. ; Howell, Leigh A. ; Ryan, Donna H. ; De Souza, Errol B. ; Harris, Ruth Babette. / The role of CRF2 receptors in corticotropin-releasing factor- and urocortin-induced anorexia. In: NeuroReport. 1998 ; Vol. 9, No. 7. pp. 1601-1606.
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