The small chemical vacuolin-1 inhibits Ca2+-dependent lysosomal exocytosis but not cell resealing

Jan Cerny, Yan Feng, Anan Yu, Katsuya Miyake, Barbara Borgonovo, Judith Klumperman, Jacopo Meldolesi, Paul L. McNeil, Tomas Kirchhausen

Research output: Contribution to journalArticle

81 Scopus citations

Abstract

Resealing after wounding, the process of repair following plasma membrane damage, requires exocytosis. Vacuolins are molecules that induce rapid formation of large, swollen structures derived from endosomes and lysosomes by homotypic fusion combined with uncontrolled fusion of the inner and limiting membranes of these organelles. Vacuolin-1, the most potent compound, blocks the Ca2+- dependent exocytosis of lysosomes induced by ionomycin or plasma membrane wounding, without affecting the process of resealing. In contrast, other cell structures and membrane trafficking functions including exocytosis of enlargeosomes are unaffected. Because cells heal normally in the presence of vacuolin-1, we suggest that lysosomes are dispensable for resealing.

Original languageEnglish (US)
Pages (from-to)883-888
Number of pages6
JournalEMBO Reports
Volume5
Issue number9
DOIs
StatePublished - Sep 2004

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

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    Cerny, J., Feng, Y., Yu, A., Miyake, K., Borgonovo, B., Klumperman, J., Meldolesi, J., McNeil, P. L., & Kirchhausen, T. (2004). The small chemical vacuolin-1 inhibits Ca2+-dependent lysosomal exocytosis but not cell resealing. EMBO Reports, 5(9), 883-888. https://doi.org/10.1038/sj.embor.7400243