The use of C0t-1 probe DNA for the detection of low levels of DNA fragmentation.

P. Wong, Sylvia B Smith, N. Bora, S. Gentleman

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In many apoptotic systems the final demise of the DNA results in the generation of multinucleosomal-sized DNA fragments, which appear as a DNA ladder after agarose electrophoresis. Extensive DNA fragmentation can be detected by ethidium bromide staining. Visualization of low levels of DNA fragmentation, however, requires both a more sensitive detection system, as well as a method of DNA extraction, that limits the extent of high molecular weight DNA shearing. We have found that the use of C0t-1 repetitive DNA as a probe for Southern analysis of DNA ladders is a sensitive method to detect low levels of DNA fragmentation. We have applied this methodology in the detection of DNA fragmentation in the normal developing C57BL/6 mouse retina at stages in which there is known DNA fragmentation, as well as in human Y-79 retinoblastoma cells grown in culture. We have found that in many instances in which there is no detectable DNA ladder with ethidium bromide staining a very definitive DNA ladder can be visualized via Southern blot analysis with a C0t-1 DNA probe.

Original languageEnglish (US)
Pages (from-to)649-653
Number of pages5
JournalBiochemistry and cell biology = Biochimie et biologie cellulaire
Volume72
Issue number11-12
DOIs
StatePublished - Jan 1 1994
Externally publishedYes

Fingerprint

DNA Probes
DNA Fragmentation
DNA
Ladders
Ethidium
Staining and Labeling
Retinoblastoma
Southern Blotting
Inbred C57BL Mouse
Sepharose
Electrophoresis
Retina
Molecular Weight
Shearing
Cell culture
Visualization

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

The use of C0t-1 probe DNA for the detection of low levels of DNA fragmentation. / Wong, P.; Smith, Sylvia B; Bora, N.; Gentleman, S.

In: Biochemistry and cell biology = Biochimie et biologie cellulaire, Vol. 72, No. 11-12, 01.01.1994, p. 649-653.

Research output: Contribution to journalArticle

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