The use of sodium trimetaphosphate as a biomimetic analog of matrix phosphoproteins for remineralization of artificial caries-like dentin

Yan Liu, Nan Li, Yipin Qi, Li Na Niu, Sally Elshafiy, Jing Mao, Lorenzo Breschi, David Henry Pashley, Franklin Chi Meng Tay

Research output: Contribution to journalArticle

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Abstract

Objectives: This study examined the use of sodium trimetaphosphate (STMP) as a biomimetic analog of matrix phosphoproteins for remineralization of artificial carious-affected dentin. Methods: Artificial carious lesions with lesion depths of 300 ± 30 μm were created by pH-cycling. 2.5% hydrolyzed STMP was applied to the artificial carious lesions to phosphorylate the partially-demineralized collagen matrix. Half of the STMP-treated specimens were bonded with One-Step. The adhesive and non-adhesive infiltrated specimens were remineralized in a Portland cement-simulated body fluid system containing polyacrylic acid (PAA) to stabilize amorphous calcium phosphate as nanoprecursors. Micro-computed tomography (micro-CT) and transmission electron microscopy (TEM) were used to evaluate the results of remineralization after a 4-month period. Results: In absence of PAA and STMP as biomimetic analogs (control groups), there was no remineralization irrespective of whether the lesions were infiltrated with adhesive. For the STMP-treated experimental groups immersed in PAA-containing simulated body fluid, specimens without adhesive infiltration were more heavily remineralized than those infiltrated with adhesive. Statistical analysis of the 4-month micro-CT data revealed significant differences in the lesion depth, relative mineral content along the lesion surface and changes in ΔZ between the non-adhesive and adhesive experimental groups (p < 0.05 for all the three parameters). TEM examination indicated that collagen degradation occurred in both the non-adhesive and adhesive control and experimental groups after 4 months of remineralization. Significance: Biomimetic remineralization using STMP is a promising method to remineralize artificial carious lesions particularly in areas devoid of seed crystallites. Future studies should consider the incorporation of MMP-inhibitors within the partially-demineralized collagen matrix to prevent collagen degradation during remineralization.

Original languageEnglish (US)
Pages (from-to)465-477
Number of pages13
JournalDental Materials
Volume27
Issue number5
DOIs
StatePublished - May 1 2011

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Biomimetics
carbopol 940
Phosphoproteins
Dentin
Adhesives
Sodium
Collagen
Body fluids
Body Fluids
Tomography
Acids
Electron Microscope Tomography
Transmission electron microscopy
Degradation
Control Groups
Matrix Metalloproteinase Inhibitors
Calcium phosphate
Dental Caries
Portland cement
Transmission Electron Microscopy

Keywords

  • Artificial carious lesion
  • Biomimetic
  • Collagen
  • Degradation
  • Remineralization
  • Sodium trimetaphosphate

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

Cite this

The use of sodium trimetaphosphate as a biomimetic analog of matrix phosphoproteins for remineralization of artificial caries-like dentin. / Liu, Yan; Li, Nan; Qi, Yipin; Niu, Li Na; Elshafiy, Sally; Mao, Jing; Breschi, Lorenzo; Pashley, David Henry; Tay, Franklin Chi Meng.

In: Dental Materials, Vol. 27, No. 5, 01.05.2011, p. 465-477.

Research output: Contribution to journalArticle

Liu, Yan ; Li, Nan ; Qi, Yipin ; Niu, Li Na ; Elshafiy, Sally ; Mao, Jing ; Breschi, Lorenzo ; Pashley, David Henry ; Tay, Franklin Chi Meng. / The use of sodium trimetaphosphate as a biomimetic analog of matrix phosphoproteins for remineralization of artificial caries-like dentin. In: Dental Materials. 2011 ; Vol. 27, No. 5. pp. 465-477.
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abstract = "Objectives: This study examined the use of sodium trimetaphosphate (STMP) as a biomimetic analog of matrix phosphoproteins for remineralization of artificial carious-affected dentin. Methods: Artificial carious lesions with lesion depths of 300 ± 30 μm were created by pH-cycling. 2.5{\%} hydrolyzed STMP was applied to the artificial carious lesions to phosphorylate the partially-demineralized collagen matrix. Half of the STMP-treated specimens were bonded with One-Step. The adhesive and non-adhesive infiltrated specimens were remineralized in a Portland cement-simulated body fluid system containing polyacrylic acid (PAA) to stabilize amorphous calcium phosphate as nanoprecursors. Micro-computed tomography (micro-CT) and transmission electron microscopy (TEM) were used to evaluate the results of remineralization after a 4-month period. Results: In absence of PAA and STMP as biomimetic analogs (control groups), there was no remineralization irrespective of whether the lesions were infiltrated with adhesive. For the STMP-treated experimental groups immersed in PAA-containing simulated body fluid, specimens without adhesive infiltration were more heavily remineralized than those infiltrated with adhesive. Statistical analysis of the 4-month micro-CT data revealed significant differences in the lesion depth, relative mineral content along the lesion surface and changes in ΔZ between the non-adhesive and adhesive experimental groups (p < 0.05 for all the three parameters). TEM examination indicated that collagen degradation occurred in both the non-adhesive and adhesive control and experimental groups after 4 months of remineralization. Significance: Biomimetic remineralization using STMP is a promising method to remineralize artificial carious lesions particularly in areas devoid of seed crystallites. Future studies should consider the incorporation of MMP-inhibitors within the partially-demineralized collagen matrix to prevent collagen degradation during remineralization.",
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AU - Elshafiy, Sally

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