Therapeutic doses of radiation alter proliferation and attachment of osteoblasts to implant surfaces

Mansur Ahmad, Christopher Sampair, Abu Nasir Mohammad Nazmul Hossain, Neerja Khurana, Andrew Nerness, Patcharaporn Wutticharoenmongkol

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Osseointegration of implants in irradiated bone is inadequate. The effect of radiation on cell-implant material interaction has not been adequately studied. The goal of this study was to investigate the effects of ionizing radiation on the proliferation, differentiation, and attachment of osteoblasts to commercially pure titanium (cpTi). Human fetal osteoblasts (hFOB) were irradiated either before or after plating in tissue culture (TC) dishes with or without cpTi disks. Radiation was single dose of 10 cGy, 25 cGy, 50 cGy, 1 Gy, 2 Gy, 4 Gy or 8 Gy. Cell proliferation was determined by counting trypsinized cells on 7 days after irradiation. Attachment of irradiated hFOB was measured indirectly by counting cells 2 and 6 h after plating. Differentiation was evaluated by alkaline phosphatase activity. Compared with nonirradiated sham controls, higher doses of radiation significantly reduced cell attachment and proliferation. Both proliferation and attachment were significantly lower on cpTi compared with TC. Attachment decreased based on the length of postirradiation period. Although differentiation was significantly enhanced by a dose of 8 Gy, proliferation was lowest. These initial studies show that effects of therapeutic doses of radiation on osteoblasts varied depending on the surface, time-elapsed, and amount of radiation.

Original languageEnglish (US)
Pages (from-to)926-934
Number of pages9
JournalJournal of Biomedical Materials Research - Part A
Volume86
Issue number4
DOIs
StatePublished - Sep 15 2008

Fingerprint

Osteoblasts
Dosimetry
Titanium
Radiation
Tissue culture
Plating
Cell Proliferation
Osseointegration
Radiation Effects
Therapeutic Uses
Therapeutics
Ionizing Radiation
Ionizing radiation
Phosphatases
Alkaline Phosphatase
Cell proliferation
Bone
Bone and Bones
Irradiation

Keywords

  • Attachment
  • Ionizing radiation
  • Osteoblasts
  • Proliferation
  • Titanium

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

Cite this

Therapeutic doses of radiation alter proliferation and attachment of osteoblasts to implant surfaces. / Ahmad, Mansur; Sampair, Christopher; Nazmul Hossain, Abu Nasir Mohammad; Khurana, Neerja; Nerness, Andrew; Wutticharoenmongkol, Patcharaporn.

In: Journal of Biomedical Materials Research - Part A, Vol. 86, No. 4, 15.09.2008, p. 926-934.

Research output: Contribution to journalArticle

Ahmad, Mansur ; Sampair, Christopher ; Nazmul Hossain, Abu Nasir Mohammad ; Khurana, Neerja ; Nerness, Andrew ; Wutticharoenmongkol, Patcharaporn. / Therapeutic doses of radiation alter proliferation and attachment of osteoblasts to implant surfaces. In: Journal of Biomedical Materials Research - Part A. 2008 ; Vol. 86, No. 4. pp. 926-934.
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