Therapeutic effect of a topical CCR2 antagonist on induced alveolar bone loss in mice

S. P. Barros, Roger Mauricio Arce Munoz, P. Galloway, R. Lawter, S. Offenbacher

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background and Objective: Chemokines are known to regulate leukocyte trafficking, recruitment and infiltration in periodontal diseases. The study objective was to determine the effect of an experimental oral/topical chemokine (C-C motif) receptor 2 (CCR2)-antagonist treatment on alveolar bone loss in a mouse model of Porphyromonas gingivalis-induced periodontitis. Material and Methods: Balb/C mice (n=41) were randomly assigned to four groups. Group1 was infected by P. gingivalis applied orally/topically for 5wk. Group2 was also infected and then treated with vehicle (aqueous methylcellulose) for an additional 4wk. Group3 was infected and orally/topically treated with CCR2 antagonist (10mg/kg). Group4 served as a noninfected, nontreated control group. Mice received intraperitoneal injections of Alizarin (30mg/kg) and calcein (20mg/kg) three times from the last day of infection to determine mineral deposition, reflecting bone dynamics. Mandibles were analysed by morphometry and confocal fluorescence microscopy. Results: Alveolar bone loss was compared among groups using Tukey's test, and bone formation was qualitatively observed. Infected mice showed significantly greater alveolar bone loss than noninfected control animals (group1 vs. 4, p<0.01). Vehicle-treated mice (group2) showed the largest area of alveolar bone loss (p<0.01), while mice treated with the CCR2 antagonist showed the smallest area of alveolar bone loss and were similar to the control group (group3 vs. 4, p=0.14). Qualitative analysis of fluorescent dye uptake indicated increased bone formation in CCR2-antagonist-treated mice, suggesting an improved bone repairing process. Conclusion: The results suggested that treatment with CCR2 antagonist inhibited alveolar bone loss and improved bone formation in this model. These data support further evaluation of CCR2 antagonist as a therapeutic target for the development of new treatment modalities on bacterially induced alveolar bone resorption.

Original languageEnglish (US)
Pages (from-to)246-251
Number of pages6
JournalJournal of Periodontal Research
Volume46
Issue number2
DOIs
StatePublished - Apr 1 2011
Externally publishedYes

Fingerprint

Alveolar Bone Loss
Therapeutic Uses
Osteogenesis
Porphyromonas gingivalis
CCR Receptors
Bone and Bones
Control Groups
Methylcellulose
Periodontitis
Periodontal Diseases
Therapeutics
Bone Resorption
Intraperitoneal Injections
Mandible
Fluorescent Dyes
Fluorescence Microscopy
Chemokines
Confocal Microscopy
Minerals
Leukocytes

Keywords

  • Alveolar bone
  • Animal model
  • Chemokine
  • Inflammation
  • Inflammatory mediator
  • in vivo model

ASJC Scopus subject areas

  • Periodontics

Cite this

Therapeutic effect of a topical CCR2 antagonist on induced alveolar bone loss in mice. / Barros, S. P.; Arce Munoz, Roger Mauricio; Galloway, P.; Lawter, R.; Offenbacher, S.

In: Journal of Periodontal Research, Vol. 46, No. 2, 01.04.2011, p. 246-251.

Research output: Contribution to journalArticle

Barros, S. P. ; Arce Munoz, Roger Mauricio ; Galloway, P. ; Lawter, R. ; Offenbacher, S. / Therapeutic effect of a topical CCR2 antagonist on induced alveolar bone loss in mice. In: Journal of Periodontal Research. 2011 ; Vol. 46, No. 2. pp. 246-251.
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