Three-dimensional structure of an immunoglobulin light-chain dimer with amyloidogenic properties

Philip C. Bourne, Paul A. Ramsland, Lin Shan, Zhao C. Fan, Christina R. DeWitt, Brandon B. Shultz, Simon S. Terzyan, Carolyn R. Moomaw, Clive A. Slaughter, Luke W. Guddat, Allen B. Edmundson

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The X-ray structure of an immunoglobulin light-chain dimer isolated from the urine as a 'Bence-Jones protein' from a patient with multiple myeloma and amyloidosis (Sea) was determined at 1.94 Å resolution and refined to R and Rfree factors of 0.22 and 0.25, respectively. This 'amyloidogenic' protein crystallized in the orthorhombic P212121 space group with unit-cell parameters a = 48.28, b = 83.32, c = 112.59 Å as determined at 100 K. In the vital organs (heart and kidneys), the equivalent of the urinary protein produced fibrillar amyloid deposits which were fatal to the patient. Compared with the amyloidogenic Mcg light-chain dimer, the Sea protein was highly soluble in aqueous solutions and only crystallized at concentrations approaching 100 mg m1-1. Both the Sea and Mcg proteins packed into crystals in highly ordered arrangements typical of strongly diffracting crystals of immunoglobulin fragments. Overall similarities and significant differences in the three-dimensional structures and crystalline properties are discussed for the Sea and Mcg Bence-Jones proteins, which together provide a generalized model of abnormalities present in λ chains, facilitating a better understanding of amyloidosis of light-chain origin (AL).

Original languageEnglish (US)
Pages (from-to)815-823
Number of pages9
JournalActa Crystallographica Section D: Biological Crystallography
Volume58
Issue number5
DOIs
StatePublished - Jan 1 2002
Externally publishedYes

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Immunoglobulin Light Chains
Oceans and Seas
Bence Jones Protein
Amyloidosis
Amyloidogenic Proteins
Light
Immunoglobulin Fragments
Proteins
R Factors
Amyloid Plaques
Multiple Myeloma
X-Rays
Urine
Kidney

ASJC Scopus subject areas

  • Structural Biology

Cite this

Bourne, P. C., Ramsland, P. A., Shan, L., Fan, Z. C., DeWitt, C. R., Shultz, B. B., ... Edmundson, A. B. (2002). Three-dimensional structure of an immunoglobulin light-chain dimer with amyloidogenic properties. Acta Crystallographica Section D: Biological Crystallography, 58(5), 815-823. https://doi.org/10.1107/S0907444902004183

Three-dimensional structure of an immunoglobulin light-chain dimer with amyloidogenic properties. / Bourne, Philip C.; Ramsland, Paul A.; Shan, Lin; Fan, Zhao C.; DeWitt, Christina R.; Shultz, Brandon B.; Terzyan, Simon S.; Moomaw, Carolyn R.; Slaughter, Clive A.; Guddat, Luke W.; Edmundson, Allen B.

In: Acta Crystallographica Section D: Biological Crystallography, Vol. 58, No. 5, 01.01.2002, p. 815-823.

Research output: Contribution to journalArticle

Bourne, PC, Ramsland, PA, Shan, L, Fan, ZC, DeWitt, CR, Shultz, BB, Terzyan, SS, Moomaw, CR, Slaughter, CA, Guddat, LW & Edmundson, AB 2002, 'Three-dimensional structure of an immunoglobulin light-chain dimer with amyloidogenic properties', Acta Crystallographica Section D: Biological Crystallography, vol. 58, no. 5, pp. 815-823. https://doi.org/10.1107/S0907444902004183
Bourne, Philip C. ; Ramsland, Paul A. ; Shan, Lin ; Fan, Zhao C. ; DeWitt, Christina R. ; Shultz, Brandon B. ; Terzyan, Simon S. ; Moomaw, Carolyn R. ; Slaughter, Clive A. ; Guddat, Luke W. ; Edmundson, Allen B. / Three-dimensional structure of an immunoglobulin light-chain dimer with amyloidogenic properties. In: Acta Crystallographica Section D: Biological Crystallography. 2002 ; Vol. 58, No. 5. pp. 815-823.
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