Thyroid hormone responsiveness is developmentally regulated in the rat small intestine: A possible role for the α-2 receptor variant

Richard A. Hodint, Shufen Meng, Sherman M. Chamberlain

Research output: Contribution to journalArticle

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Abstract

Thyroid hormone (T3) alters gene expression through binding to a receptor protein located within the nucleus of target cells. Multiple forms of the T3 receptor (TR) have been identified and are encoded by the α and β c-erbA genes. We have previously found that TR β-1 is the major receptor form expressed in the adult rat small intestine, although there are also moderate levels of c-erbA α-2, a nonhormone-binding variant that is thought to inhibit T3 action. In developing rats, we studied the regulation of two small intestinal enterocyte genes previously shown to be T3 responsive, lactase and 3.0-kilobase intestinal alkaline phosphatase (IAP). Animals were treated with six daily ip injections of either saline (control) or 30 μgrams/100 g BW T3 (T3 group) and killed at 10 and 25 days of age. Northern analyses of RNA derived from intestinal tissues showed that the magnitude of the T3-induced changes in lactase and IAP gene expression increased with development. Jejunal 3.0-kilobase IAP messenger RNA (mRNA) levels were unaffected by T3 at 10 days, but increased by 15-fold at 25 days. Similarly, jejunal lactase mRNA levels were unchanged by T3 at 10 days, but decreased by 75% at 25 days. Qualitatively similar results were seen in the duodenum and ileum. Studies of TR expression revealed that TR β-1 mRNA levels were unchanged during the developmental period, whereas the levels of c-erbA α-2 decreased by 90% between 5-25 days after birth. These results indicate that the rat small intestine becomes increasingly T3 responsive during postnatal development. These changes occur in parallel with a decline in c-erbA α-2 levels, suggesting that this T3 receptor variant may play a role in this hormonal responsiveness.

Original languageEnglish (US)
Pages (from-to)564-568
Number of pages5
JournalEndocrinology
Volume135
Issue number2
DOIs
StatePublished - Aug 1994

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Thyroid Hormone Receptors
Thyroid Hormones
Small Intestine
Lactase
Alkaline Phosphatase
Messenger RNA
erbA Genes
Gene Expression
Enterocytes
Triiodothyronine
Cell Nucleus
Ileum
Duodenum
Parturition
RNA
Injections
Genes
Proteins

ASJC Scopus subject areas

  • Endocrinology

Cite this

Thyroid hormone responsiveness is developmentally regulated in the rat small intestine : A possible role for the α-2 receptor variant. / Hodint, Richard A.; Meng, Shufen; Chamberlain, Sherman M.

In: Endocrinology, Vol. 135, No. 2, 08.1994, p. 564-568.

Research output: Contribution to journalArticle

Hodint, Richard A. ; Meng, Shufen ; Chamberlain, Sherman M. / Thyroid hormone responsiveness is developmentally regulated in the rat small intestine : A possible role for the α-2 receptor variant. In: Endocrinology. 1994 ; Vol. 135, No. 2. pp. 564-568.
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