Tie2cre-induced inactivation of the miRNA-processing enzyme Dicer disrupts invariant NKT cell development

Li Zhou, Kook Heon Seo, Hong Zhi He, Rafal W Pacholczyk, Dong Mei Meng, Chang Gui Li, Jianrui Xu, Jin-Xiong She, Zheng Dong, Qing Sheng Mi

Research output: Contribution to journalArticle

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Abstract

MicroRNAs (miRNAs) are a class of evolutionarily conserved small noncoding RNAs that are increasingly being recognized as important regulators of gene expression. The ribonuclease III enzyme Dicer is essential for the processing of miRNAs. CD1d-restricted invariant natural killer T (iNKT) cells are potent regulators of diverse immune responses. The role of Dicer-generated miRNAs in the development and function of immune regulatory iNKT cells is unknown. Here, we generated a mouse strain with a tissue-specific disruption of Dicer, and showed that lack of miRNAs after the deletion of Dicer by Tie2-Cre (expressed in hematopoietic cells and endothelial cells) interrupted the development and maturation of iNKT cells in the thymus and significantly decreased the number of iNKT cells in different immune organs. Thymic and peripheral iNKT cell compartments were changed in miRNA-deficient mice, with a significantly increased frequency of CD4+CD8+ iNKT cells in the thymus and a significantly decreased frequency of CD4+ iNKT cells in the spleen. MiRNA-deficient iNKT cells display profound defects in α-GalCer-induced activation and cytokine production. Bone marrow (BM) from miRNA-deficient mice poorly reconstituted iNKT cells compared to BM from WT mice. Also, using a thymic iNKT cell transfer model, we found that iNKT cell homeostasis was impaired in miRNA-deficient recipient mice. Our data indicate that miRNAs expressed in hematopoietic cells and endothelial cells are potent regulators of iNKT cell development, function, and homeostasis.

Original languageEnglish (US)
Pages (from-to)10266-10271
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume106
Issue number25
DOIs
StatePublished - Jun 23 2009

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Ribonuclease III
Natural Killer T-Cells
MicroRNAs
Thymus Gland
Homeostasis
Endothelial Cells
Bone Marrow
Small Untranslated RNA
Regulator Genes

Keywords

  • Bone marrow
  • Galactosylceramide
  • T cell
  • Thymus

ASJC Scopus subject areas

  • General

Cite this

Tie2cre-induced inactivation of the miRNA-processing enzyme Dicer disrupts invariant NKT cell development. / Zhou, Li; Seo, Kook Heon; He, Hong Zhi; Pacholczyk, Rafal W; Meng, Dong Mei; Li, Chang Gui; Xu, Jianrui; She, Jin-Xiong; Dong, Zheng; Mi, Qing Sheng.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, No. 25, 23.06.2009, p. 10266-10271.

Research output: Contribution to journalArticle

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