TY - JOUR
T1 - Tissue-specific expression, hormonal regulation and 5′-flanking gene region of the rat clara cell 10 kDa protein
T2 - Comparison to rabbit uteroglobin
AU - Hagen, Gustav
AU - Wolf, Markus
AU - Katyal, Sikandar L.
AU - Singh, Gurmukh
AU - Beato, Miguel
AU - Suske, Guntram
N1 - Funding Information:
We thank Dr. H. Bruller for the synthesis of oligonucleotides. Drs. R.Hache\ M.Kalff and E.Slater are gratefully acknowledged for critical reading of the manuscript. This work was supported by grants from the Deutsche Forschungsgemeinschaft (SFB215/B3 and Mu601/5-l).
PY - 1990/5/25
Y1 - 1990/5/25
N2 - The amino acid sequence of rat Clara Cell 10 kDa secretory protein (CC10) shows 55% identity to rabbit uteroglobin. In order to define the relationship between rat CC10 and rabbit uteroglobin in detail, the tissuespecific expression and hormonal regulation of rat CC10 mRNA was analyzed. We report that like rabbit uteroglobin, rat CC10 mRNA is expressed in lung and esophagus, as well as in uteri of estrogen- and progesterone-treated females. Expression of CC10 mRNA in lung is regulated by glucocorticoids. The similarity in expression pattern of rat CC10 mRNA and rabbit uteroglobin mRNA is reflected by a striking similarity in the 5′-flanking regions of the two genes. Despite this overall similarity, two regions of 0.3 kb and 2.1 kb are absent in the rat CC10 upstream gene region. The larger region includes a cluster of hormone receptor binding sites, believed to be responsible for differential regulation of rabbit uteroglobin by glucocorticoids and progesterone. Thus, while the sequence identities in the coding and 5′-flanking regions point towards a common ancestor for the uteroglobin and CC10 gene, later events (deletions/insertions) might have caused speciesspecific differences In their regulation.
AB - The amino acid sequence of rat Clara Cell 10 kDa secretory protein (CC10) shows 55% identity to rabbit uteroglobin. In order to define the relationship between rat CC10 and rabbit uteroglobin in detail, the tissuespecific expression and hormonal regulation of rat CC10 mRNA was analyzed. We report that like rabbit uteroglobin, rat CC10 mRNA is expressed in lung and esophagus, as well as in uteri of estrogen- and progesterone-treated females. Expression of CC10 mRNA in lung is regulated by glucocorticoids. The similarity in expression pattern of rat CC10 mRNA and rabbit uteroglobin mRNA is reflected by a striking similarity in the 5′-flanking regions of the two genes. Despite this overall similarity, two regions of 0.3 kb and 2.1 kb are absent in the rat CC10 upstream gene region. The larger region includes a cluster of hormone receptor binding sites, believed to be responsible for differential regulation of rabbit uteroglobin by glucocorticoids and progesterone. Thus, while the sequence identities in the coding and 5′-flanking regions point towards a common ancestor for the uteroglobin and CC10 gene, later events (deletions/insertions) might have caused speciesspecific differences In their regulation.
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U2 - 10.1093/nar/18.10.2939
DO - 10.1093/nar/18.10.2939
M3 - Article
C2 - 2349092
AN - SCOPUS:0025345772
SN - 0305-1048
VL - 18
SP - 2939
EP - 2946
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 10
ER -