Tissue-specific expression in transgenic mice directed by the 5'-flanking sequences of the human gene encoding interphotoreceptor retinoid-binding protein

G. I. Liou, L. Geng, M. R. Al-Ubaidi, S. Matragoon, G. Hanten, W. Baehr, P. A. Overbeek

Research output: Contribution to journalArticle

56 Scopus citations

Abstract

Interphotoreceptor retinoid-binding protein (IRBP) is an extracellular protein that has been suggested to participate in the visual process as a carrier for visual retinoids. A chimeric gene composed of the human IRBP promoter fused to the bacterial reporter gene chloramphenicol acetyltransferase (CAT) was used to generate transgenic mice. Analysis of six transgenic families revealed that the CAT gene, concomitant with the endogenous IRBP gene, was expressed primarily in the retina and, to a lesser extent, in the pineal gland. These results establish that a 1.3-kilobase fragment from the 5' end of the human IRBP gene is sufficient to direct transgene expression to a visual subdivision of the central nervous system.

Original languageEnglish (US)
Pages (from-to)8373-8376
Number of pages4
JournalJournal of Biological Chemistry
Volume265
Issue number15
StatePublished - Jun 12 1990

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Liou, G. I., Geng, L., Al-Ubaidi, M. R., Matragoon, S., Hanten, G., Baehr, W., & Overbeek, P. A. (1990). Tissue-specific expression in transgenic mice directed by the 5'-flanking sequences of the human gene encoding interphotoreceptor retinoid-binding protein. Journal of Biological Chemistry, 265(15), 8373-8376.