Tissue-specific expression in transgenic mice directed by the 5'-flanking sequences of the human gene encoding interphotoreceptor retinoid-binding protein

G. I. Liou, L. Geng, M. R. Al-Ubaidi, S. Matragoon, G. Hanten, W. Baehr, P. A. Overbeek

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Interphotoreceptor retinoid-binding protein (IRBP) is an extracellular protein that has been suggested to participate in the visual process as a carrier for visual retinoids. A chimeric gene composed of the human IRBP promoter fused to the bacterial reporter gene chloramphenicol acetyltransferase (CAT) was used to generate transgenic mice. Analysis of six transgenic families revealed that the CAT gene, concomitant with the endogenous IRBP gene, was expressed primarily in the retina and, to a lesser extent, in the pineal gland. These results establish that a 1.3-kilobase fragment from the 5' end of the human IRBP gene is sufficient to direct transgene expression to a visual subdivision of the central nervous system.

Original languageEnglish (US)
Pages (from-to)8373-8376
Number of pages4
JournalJournal of Biological Chemistry
Volume265
Issue number15
StatePublished - Jun 12 1990

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Gene encoding
5' Flanking Region
Transgenic Mice
Genes
Tissue
Chloramphenicol O-Acetyltransferase
Bacterial Genes
Pineal Gland
Retinoids
Transgenes
Reporter Genes
Retina
Neurology
Central Nervous System
interstitial retinol-binding protein
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Liou, G. I., Geng, L., Al-Ubaidi, M. R., Matragoon, S., Hanten, G., Baehr, W., & Overbeek, P. A. (1990). Tissue-specific expression in transgenic mice directed by the 5'-flanking sequences of the human gene encoding interphotoreceptor retinoid-binding protein. Journal of Biological Chemistry, 265(15), 8373-8376.

Tissue-specific expression in transgenic mice directed by the 5'-flanking sequences of the human gene encoding interphotoreceptor retinoid-binding protein. / Liou, G. I.; Geng, L.; Al-Ubaidi, M. R.; Matragoon, S.; Hanten, G.; Baehr, W.; Overbeek, P. A.

In: Journal of Biological Chemistry, Vol. 265, No. 15, 12.06.1990, p. 8373-8376.

Research output: Contribution to journalArticle

Liou, GI, Geng, L, Al-Ubaidi, MR, Matragoon, S, Hanten, G, Baehr, W & Overbeek, PA 1990, 'Tissue-specific expression in transgenic mice directed by the 5'-flanking sequences of the human gene encoding interphotoreceptor retinoid-binding protein', Journal of Biological Chemistry, vol. 265, no. 15, pp. 8373-8376.
Liou, G. I. ; Geng, L. ; Al-Ubaidi, M. R. ; Matragoon, S. ; Hanten, G. ; Baehr, W. ; Overbeek, P. A. / Tissue-specific expression in transgenic mice directed by the 5'-flanking sequences of the human gene encoding interphotoreceptor retinoid-binding protein. In: Journal of Biological Chemistry. 1990 ; Vol. 265, No. 15. pp. 8373-8376.
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