TNF-α inhibition attenuates adverse myocardial remodeling in a rat model of volume overload

Lynetta Johnson Jobe, Giselle C. Meléndez, Scott P. Levick, Yan Du, Gregory L. Brower, Joseph S. Janicki

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Tumor necrosis factor (TNF)-α is a proinflammatory cytokine that has been implicated in the pathogenesis of heart failure. In contrast, we have recently shown that myocardial levels of TNF-α are acutely elevated in the aortocaval (AV) fistula model of heart failure. Based on these observations, we hypothesized that progression of adverse myocardial remodeling secondary to volume overload would be prevented by inhibition of TNF-α with etanercept. Furthermore, a principal objective of this study was to elucidate the effect of TNF-α inhibition during different phases of the myocardial remodeling process. Eight-week-old male Sprague-Dawley rats were randomly divided into the following three groups: sham-operated controls, untreated AV fistulas, and etanercept-treated AV fistulas. Each group was further subdivided to study three different time points consisting of 3 days, 3 wk, and 8 wk postfistula. Etanercept was administered subcutaneously at 1 mg/kg body wt. Etanercept prevented collagen degradation at 3 days and significantly attenuated the decrease in collagen at 8 wk postfistula. Although TNF-α antagonism did not prevent the initial ventricular dilatation at 3 wk postfistula, etanercept was effective at significantly attenuating the subsequent ventricular hypertrophy, dilatation, and increased compliance at 8 wk postfistula. These positive adaptations achieved with etanercept administration translated into significant functional improvements. At a cellular level, etanercept also markedly attenuated increases in cardiomyocyte length, width, and area at 8 wk postfistula. These observations demonstrate that TNF-α has a pivotal role in adverse myocardial remodeling and that treatment with etanercept can attenuate the progression to heart failure.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume297
Issue number4
DOIs
StatePublished - Oct 1 2009
Externally publishedYes

Fingerprint

Tumor Necrosis Factor-alpha
Fistula
Heart Failure
Dilatation
Collagen
Etanercept
Cardiac Myocytes
Hypertrophy
Compliance
Sprague Dawley Rats
Cytokines

Keywords

  • Diastolic function
  • Etanercept
  • Extracellular matrix
  • Intrinsic contractility
  • Myocyte dimensions
  • Ventricular pressure-volume relationship

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

TNF-α inhibition attenuates adverse myocardial remodeling in a rat model of volume overload. / Jobe, Lynetta Johnson; Meléndez, Giselle C.; Levick, Scott P.; Du, Yan; Brower, Gregory L.; Janicki, Joseph S.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 297, No. 4, 01.10.2009.

Research output: Contribution to journalArticle

Jobe, Lynetta Johnson ; Meléndez, Giselle C. ; Levick, Scott P. ; Du, Yan ; Brower, Gregory L. ; Janicki, Joseph S. / TNF-α inhibition attenuates adverse myocardial remodeling in a rat model of volume overload. In: American Journal of Physiology - Heart and Circulatory Physiology. 2009 ; Vol. 297, No. 4.
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