TNP-ATP-resistant P2X ionic current on the central terminals and somata of rat primary sensory neurons

Kenzo Tsuzuki, Ariel Ase, Philippe Séguéla, Terumasa Nakatsuka, Cong Yi Wang, Jin-Xiong She, Jianguo G. Gu

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

P2X receptors have been suggested to be expressed on the central terminals of Aδ-afferent fibers innervating dorsal horn lamina V and play a role in modulating sensory synaptic transmission. These P2X receptors have been widely thought to be P2X2+3 receptors. However, we have recently found that P2X receptor-mediated modulation of sensory transmission in lamina V is not inhibited by trinitrophenyl-adenosine triphosphate (TNP-ATP), a potent antagonist of P2X1, P2X3 homomers, and P2X2+3 heteromers. To provide direct evidence for the presence of TNP-ATP-resistant P2X receptors on primary afferent fibers, we examined α,β-methylene-ATP (αβmeATP)-evoked currents and their sensitivity to TNP-ATP in rat dorsal root ganglion (DRG) neurons, αβmeATP evoked fast currents, slow currents, and mixed currents that contained both fast and slow current-components. Fast currents and fast current components in the mixed currents were both completely inhibited by 0.1 μM TNP-ATP (n = 14). Both slow currents and slow-current components in the mixed currents showed broad spectrum of sensitivity to 1 μM TNP-ATP, ranging from complete block (TNP-ATP-sensitive) to little block (TNP-ATP-resistant). TNP-ATP-resistant currents evoked by 10 μM αβmeATP could be largely inhibited by 10 μM iso-pyridox-alphosphate-6-azophenyl-2′,4′-disulphonic acid. Cells with P2X currents that were highly resistant to TNP-ATP were found to be insensitive to capsaicin. These results suggest that TNP-ATP-resistant P2X receptor subtypes are expressed on capsaicin-insensitive Aδ-afferent fibers and play a role in modulating sensory transmission to lamina V neurons.

Original languageEnglish (US)
Pages (from-to)3235-3242
Number of pages8
JournalJournal of Neurophysiology
Volume89
Issue number6
DOIs
StatePublished - Jun 1 2003

Fingerprint

Carisoprodol
Sensory Receptor Cells
Adenosine Triphosphate
Capsaicin
Purinergic P2X2 Receptors
Neurons
Spinal Ganglia
Synaptic Transmission

ASJC Scopus subject areas

  • Neuroscience(all)
  • Physiology

Cite this

TNP-ATP-resistant P2X ionic current on the central terminals and somata of rat primary sensory neurons. / Tsuzuki, Kenzo; Ase, Ariel; Séguéla, Philippe; Nakatsuka, Terumasa; Wang, Cong Yi; She, Jin-Xiong; Gu, Jianguo G.

In: Journal of Neurophysiology, Vol. 89, No. 6, 01.06.2003, p. 3235-3242.

Research output: Contribution to journalArticle

Tsuzuki, Kenzo ; Ase, Ariel ; Séguéla, Philippe ; Nakatsuka, Terumasa ; Wang, Cong Yi ; She, Jin-Xiong ; Gu, Jianguo G. / TNP-ATP-resistant P2X ionic current on the central terminals and somata of rat primary sensory neurons. In: Journal of Neurophysiology. 2003 ; Vol. 89, No. 6. pp. 3235-3242.
@article{b2dd196ff28f47aa80c9a981f28cad66,
title = "TNP-ATP-resistant P2X ionic current on the central terminals and somata of rat primary sensory neurons",
abstract = "P2X receptors have been suggested to be expressed on the central terminals of Aδ-afferent fibers innervating dorsal horn lamina V and play a role in modulating sensory synaptic transmission. These P2X receptors have been widely thought to be P2X2+3 receptors. However, we have recently found that P2X receptor-mediated modulation of sensory transmission in lamina V is not inhibited by trinitrophenyl-adenosine triphosphate (TNP-ATP), a potent antagonist of P2X1, P2X3 homomers, and P2X2+3 heteromers. To provide direct evidence for the presence of TNP-ATP-resistant P2X receptors on primary afferent fibers, we examined α,β-methylene-ATP (αβmeATP)-evoked currents and their sensitivity to TNP-ATP in rat dorsal root ganglion (DRG) neurons, αβmeATP evoked fast currents, slow currents, and mixed currents that contained both fast and slow current-components. Fast currents and fast current components in the mixed currents were both completely inhibited by 0.1 μM TNP-ATP (n = 14). Both slow currents and slow-current components in the mixed currents showed broad spectrum of sensitivity to 1 μM TNP-ATP, ranging from complete block (TNP-ATP-sensitive) to little block (TNP-ATP-resistant). TNP-ATP-resistant currents evoked by 10 μM αβmeATP could be largely inhibited by 10 μM iso-pyridox-alphosphate-6-azophenyl-2′,4′-disulphonic acid. Cells with P2X currents that were highly resistant to TNP-ATP were found to be insensitive to capsaicin. These results suggest that TNP-ATP-resistant P2X receptor subtypes are expressed on capsaicin-insensitive Aδ-afferent fibers and play a role in modulating sensory transmission to lamina V neurons.",
author = "Kenzo Tsuzuki and Ariel Ase and Philippe S{\'e}gu{\'e}la and Terumasa Nakatsuka and Wang, {Cong Yi} and Jin-Xiong She and Gu, {Jianguo G.}",
year = "2003",
month = "6",
day = "1",
doi = "10.1152/jn.01171.2002",
language = "English (US)",
volume = "89",
pages = "3235--3242",
journal = "Journal of Neurophysiology",
issn = "0022-3077",
publisher = "American Physiological Society",
number = "6",

}

TY - JOUR

T1 - TNP-ATP-resistant P2X ionic current on the central terminals and somata of rat primary sensory neurons

AU - Tsuzuki, Kenzo

AU - Ase, Ariel

AU - Séguéla, Philippe

AU - Nakatsuka, Terumasa

AU - Wang, Cong Yi

AU - She, Jin-Xiong

AU - Gu, Jianguo G.

PY - 2003/6/1

Y1 - 2003/6/1

N2 - P2X receptors have been suggested to be expressed on the central terminals of Aδ-afferent fibers innervating dorsal horn lamina V and play a role in modulating sensory synaptic transmission. These P2X receptors have been widely thought to be P2X2+3 receptors. However, we have recently found that P2X receptor-mediated modulation of sensory transmission in lamina V is not inhibited by trinitrophenyl-adenosine triphosphate (TNP-ATP), a potent antagonist of P2X1, P2X3 homomers, and P2X2+3 heteromers. To provide direct evidence for the presence of TNP-ATP-resistant P2X receptors on primary afferent fibers, we examined α,β-methylene-ATP (αβmeATP)-evoked currents and their sensitivity to TNP-ATP in rat dorsal root ganglion (DRG) neurons, αβmeATP evoked fast currents, slow currents, and mixed currents that contained both fast and slow current-components. Fast currents and fast current components in the mixed currents were both completely inhibited by 0.1 μM TNP-ATP (n = 14). Both slow currents and slow-current components in the mixed currents showed broad spectrum of sensitivity to 1 μM TNP-ATP, ranging from complete block (TNP-ATP-sensitive) to little block (TNP-ATP-resistant). TNP-ATP-resistant currents evoked by 10 μM αβmeATP could be largely inhibited by 10 μM iso-pyridox-alphosphate-6-azophenyl-2′,4′-disulphonic acid. Cells with P2X currents that were highly resistant to TNP-ATP were found to be insensitive to capsaicin. These results suggest that TNP-ATP-resistant P2X receptor subtypes are expressed on capsaicin-insensitive Aδ-afferent fibers and play a role in modulating sensory transmission to lamina V neurons.

AB - P2X receptors have been suggested to be expressed on the central terminals of Aδ-afferent fibers innervating dorsal horn lamina V and play a role in modulating sensory synaptic transmission. These P2X receptors have been widely thought to be P2X2+3 receptors. However, we have recently found that P2X receptor-mediated modulation of sensory transmission in lamina V is not inhibited by trinitrophenyl-adenosine triphosphate (TNP-ATP), a potent antagonist of P2X1, P2X3 homomers, and P2X2+3 heteromers. To provide direct evidence for the presence of TNP-ATP-resistant P2X receptors on primary afferent fibers, we examined α,β-methylene-ATP (αβmeATP)-evoked currents and their sensitivity to TNP-ATP in rat dorsal root ganglion (DRG) neurons, αβmeATP evoked fast currents, slow currents, and mixed currents that contained both fast and slow current-components. Fast currents and fast current components in the mixed currents were both completely inhibited by 0.1 μM TNP-ATP (n = 14). Both slow currents and slow-current components in the mixed currents showed broad spectrum of sensitivity to 1 μM TNP-ATP, ranging from complete block (TNP-ATP-sensitive) to little block (TNP-ATP-resistant). TNP-ATP-resistant currents evoked by 10 μM αβmeATP could be largely inhibited by 10 μM iso-pyridox-alphosphate-6-azophenyl-2′,4′-disulphonic acid. Cells with P2X currents that were highly resistant to TNP-ATP were found to be insensitive to capsaicin. These results suggest that TNP-ATP-resistant P2X receptor subtypes are expressed on capsaicin-insensitive Aδ-afferent fibers and play a role in modulating sensory transmission to lamina V neurons.

UR - http://www.scopus.com/inward/record.url?scp=0038210244&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0038210244&partnerID=8YFLogxK

U2 - 10.1152/jn.01171.2002

DO - 10.1152/jn.01171.2002

M3 - Article

C2 - 12783957

AN - SCOPUS:0038210244

VL - 89

SP - 3235

EP - 3242

JO - Journal of Neurophysiology

JF - Journal of Neurophysiology

SN - 0022-3077

IS - 6

ER -