Tolerogenic function of dimeric forms of HLA-g recombinant proteins: A comparative study in Vivo

Benoit Favier, Kiave Yune HoWangYin, Juan Wu, Julien Caumartin, Marina Daouya, Anatolij Horuzsko, Edgardo D. Carosella, Joel Le Maoult

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

HLA-G is a natural tolerogenic molecule involved in the best example of tolerance to foreign tissues there is: the maternal-fetal tolerance. The further involvement of HLA-G in the tolerance of allogeneic transplants has also been demonstrated and some of its mechanisms of action have been elucidated. For these reasons, therapeutic HLA-G molecules for tolerance induction in transplantation are actively investigated. In the present study, we studied the tolerogenic functions of three different HLA-G recombinant proteins: HLA-G heavy chain fused to β2-microglobulin (B2M), HLA-G heavy chain fused to B2M and to the Fc portion of an immunoglobulin, and HLA-G alpha-1 domain either fused to the Fc part of an immunoglobulin or as a synthetic peptide. Our results demonstrate the tolerogenic function of B2M-HLA-G fusion proteins, and especially of B2M-HLA-G5, which were capable of significantly delaying allogeneic skin graft rejection in a murine in vivo transplantation model. The results from our studies suggest that HLA-G recombinant proteins are relevant candidates for tolerance induction in human transplantation.

Original languageEnglish (US)
Article numbere21011
JournalPloS one
Volume6
Issue number7
DOIs
StatePublished - Jul 19 2011

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HLA-G Antigens
Staphylococcal Protein A
in vivo studies
Recombinant Proteins
recombinant proteins
immunoglobulins
graft rejection
synthetic peptides
skin (animal)
mechanism of action
therapeutics
mice
Immunoglobulin Fc Fragments
Transplantation
proteins
Transplantation (surgical)
Transplants
Molecules
Graft Rejection
Grafts

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Tolerogenic function of dimeric forms of HLA-g recombinant proteins : A comparative study in Vivo. / Favier, Benoit; HoWangYin, Kiave Yune; Wu, Juan; Caumartin, Julien; Daouya, Marina; Horuzsko, Anatolij; Carosella, Edgardo D.; Le Maoult, Joel.

In: PloS one, Vol. 6, No. 7, e21011, 19.07.2011.

Research output: Contribution to journalArticle

Favier, B, HoWangYin, KY, Wu, J, Caumartin, J, Daouya, M, Horuzsko, A, Carosella, ED & Le Maoult, J 2011, 'Tolerogenic function of dimeric forms of HLA-g recombinant proteins: A comparative study in Vivo', PloS one, vol. 6, no. 7, e21011. https://doi.org/10.1371/journal.pone.0021011
Favier, Benoit ; HoWangYin, Kiave Yune ; Wu, Juan ; Caumartin, Julien ; Daouya, Marina ; Horuzsko, Anatolij ; Carosella, Edgardo D. ; Le Maoult, Joel. / Tolerogenic function of dimeric forms of HLA-g recombinant proteins : A comparative study in Vivo. In: PloS one. 2011 ; Vol. 6, No. 7.
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