Topology and patch-clamp analysis of the sodium channel in relationship to the anti-lipid A antibody in campylobacteriosis

Seigo Usuki, Yoshihiko Nakatani, Kyoji Taguchi, Tetsuhiro Fujita, Shinya Tanabe, Iku Ustunomiya, Yihua Gu, Shaun A. Cawthraw, Diane G. Newell, Mohanasundari Pajaniappan, Stuart A. Thompson, Toshio Ariga, Robert K. Yu

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

An infecting strain VLA2/18 of Campylobacter jejuni was obtained from an individual with campylobacteriosis and used to prepare chicken sera by experimental infection to investigate the role of serum anti-ganglioside antibodies in Guillain-Barré syndrome. Both sera of the patient and chicken contained anti-ganglioside antibodies and anti-Lipid A (anti-Kdo2-Lipid A) antibodies directed against the lipid A portion of the bacterial lipooligosaccharide. The anti-Kdo2-Lipid A activities inhibited voltage-gated Na (Nav) channel of NSC-34 cells in culture. We hypothesized that anti-Kdo2-Lipid A antibody acts on the functional inhibition of Nav1.4. To test this possibility, a rabbit peptide antibody (anti-Nav1.4 pAb) against a 19-mer peptide (KELKDNHILNHVGLTDGPR) on the α subunit of Nav1.4 was produced. Anti-Nav1.4 pAb was cross-reactive to Kdo2-Lipid A. Anti-Kdo2-lipid A antibody activity in the chicken serum was tested for the Na+ current inhibition in NSC-34 cells in combination with μ-Conotoxin and tetrodotoxin. Contrary to our expectations, the anti-Kdo2-Lipid A antibody activity was extended to Nav channels other than Nav1.4. By overlapping structural analysis, it was found that there might be multiple peptide epitopes containing certain dipeptides showing a structural similarity with v-Lipid A. Thus, our study suggests the possibility that there are multiple epitopic peptides on the extracellular domains of Nav1.1 to 1.9, and some of them may represent target sites for anti-Kdo2-Lipid A antibody, to induce neurophysiological changes in GBS by disrupting the normal function of the Nav channels.

Original languageEnglish (US)
Pages (from-to)3359-3374
Number of pages16
JournalJournal of Neuroscience Research
Volume86
Issue number15
DOIs
StatePublished - Dec 8 2008

Fingerprint

Lipid A
Sodium Channels
Antibodies
Anti-Idiotypic Antibodies
Chickens
Peptides
Gangliosides
Serum
Conotoxins
Campylobacter jejuni
Dipeptides
Tetrodotoxin
Kdo2-lipid A
Epitopes
Cell Culture Techniques
Rabbits
Infection

Keywords

  • Campylobacter jejuni
  • Chicken
  • Ganglioside
  • Guillain-Barrée syndrome
  • Lipooligosaccharide
  • Sodium channel

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Topology and patch-clamp analysis of the sodium channel in relationship to the anti-lipid A antibody in campylobacteriosis. / Usuki, Seigo; Nakatani, Yoshihiko; Taguchi, Kyoji; Fujita, Tetsuhiro; Tanabe, Shinya; Ustunomiya, Iku; Gu, Yihua; Cawthraw, Shaun A.; Newell, Diane G.; Pajaniappan, Mohanasundari; Thompson, Stuart A.; Ariga, Toshio; Yu, Robert K.

In: Journal of Neuroscience Research, Vol. 86, No. 15, 08.12.2008, p. 3359-3374.

Research output: Contribution to journalArticle

Usuki, S, Nakatani, Y, Taguchi, K, Fujita, T, Tanabe, S, Ustunomiya, I, Gu, Y, Cawthraw, SA, Newell, DG, Pajaniappan, M, Thompson, SA, Ariga, T & Yu, RK 2008, 'Topology and patch-clamp analysis of the sodium channel in relationship to the anti-lipid A antibody in campylobacteriosis', Journal of Neuroscience Research, vol. 86, no. 15, pp. 3359-3374. https://doi.org/10.1002/jnr.21781
Usuki, Seigo ; Nakatani, Yoshihiko ; Taguchi, Kyoji ; Fujita, Tetsuhiro ; Tanabe, Shinya ; Ustunomiya, Iku ; Gu, Yihua ; Cawthraw, Shaun A. ; Newell, Diane G. ; Pajaniappan, Mohanasundari ; Thompson, Stuart A. ; Ariga, Toshio ; Yu, Robert K. / Topology and patch-clamp analysis of the sodium channel in relationship to the anti-lipid A antibody in campylobacteriosis. In: Journal of Neuroscience Research. 2008 ; Vol. 86, No. 15. pp. 3359-3374.
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abstract = "An infecting strain VLA2/18 of Campylobacter jejuni was obtained from an individual with campylobacteriosis and used to prepare chicken sera by experimental infection to investigate the role of serum anti-ganglioside antibodies in Guillain-Barr{\'e} syndrome. Both sera of the patient and chicken contained anti-ganglioside antibodies and anti-Lipid A (anti-Kdo2-Lipid A) antibodies directed against the lipid A portion of the bacterial lipooligosaccharide. The anti-Kdo2-Lipid A activities inhibited voltage-gated Na (Nav) channel of NSC-34 cells in culture. We hypothesized that anti-Kdo2-Lipid A antibody acts on the functional inhibition of Nav1.4. To test this possibility, a rabbit peptide antibody (anti-Nav1.4 pAb) against a 19-mer peptide (KELKDNHILNHVGLTDGPR) on the α subunit of Nav1.4 was produced. Anti-Nav1.4 pAb was cross-reactive to Kdo2-Lipid A. Anti-Kdo2-lipid A antibody activity in the chicken serum was tested for the Na+ current inhibition in NSC-34 cells in combination with μ-Conotoxin and tetrodotoxin. Contrary to our expectations, the anti-Kdo2-Lipid A antibody activity was extended to Nav channels other than Nav1.4. By overlapping structural analysis, it was found that there might be multiple peptide epitopes containing certain dipeptides showing a structural similarity with v-Lipid A. Thus, our study suggests the possibility that there are multiple epitopic peptides on the extracellular domains of Nav1.1 to 1.9, and some of them may represent target sites for anti-Kdo2-Lipid A antibody, to induce neurophysiological changes in GBS by disrupting the normal function of the Nav channels.",
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AU - Fujita, Tetsuhiro

AU - Tanabe, Shinya

AU - Ustunomiya, Iku

AU - Gu, Yihua

AU - Cawthraw, Shaun A.

AU - Newell, Diane G.

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