Toward gene therapy of endometriosis: transductional and transcriptional targeting of adenoviral vectors to endometriosis cells

Essam Eldin R. Othman, Zeng B. Zhu, David T. Curiel, Nilufar Khatoon, Hosam T. Salem, Essam Al Din M. Khalifa, Ayman Al-Hendy

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Objective: The purpose of this study was to screen a panel of targeted adenoviruses as vectors for endometriosis gene therapy. Study Design: Endometriotic cells were obtained from subjects with ovarian endometriomas. Liver tissues were taken from donors during hepatic transplantation surgery. Human endometriotic cells and liver tissues were transfected by targeted adenoviruses expressing luciferase reporter gene. Luciferase activity that was mediated by each virus was expressed as a percentage of adenovirus serotype 5 (Ad5-CMV-luc) activity. The 2-tailed Studentt test was used to compare the adenovirus data. Results: In endometriotic cells, the adenovirus-RGD (Ad-RGD-luc), adenovirus under secretory leukocyte protease inhibitor promoter (Ad-SLPI-luc), and adenovirus under heparanase promoter (Ad-heparanase-luc) showed significantly higher activity, compared with the adenovirus serotype 5. In liver tissues, adenovirus-survivin (Ad-survivin-luc) and Ad-heparanase-luc had significantly lower activity, compared with adenovirus serotype 5. Conclusion: Ad-heparanase-luc showed "endometriosis on, liver off" phenotype and is a promising vector for endometriosis gene therapy.

Original languageEnglish (US)
Pages (from-to)117.e1-117.e6
JournalAmerican Journal of Obstetrics and Gynecology
Volume199
Issue number2
DOIs
StatePublished - Jan 1 2008

Fingerprint

Endometriosis
Adenoviridae
Genetic Therapy
Liver
Luciferases
Secretory Leukocyte Peptidase Inhibitor
Reporter Genes
Liver Transplantation
Viruses
Phenotype
heparanase

Keywords

  • adenovirus
  • endometriosis
  • gene therapy
  • targeting strategy

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Othman, E. E. R., Zhu, Z. B., Curiel, D. T., Khatoon, N., Salem, H. T., Khalifa, E. A. D. M., & Al-Hendy, A. (2008). Toward gene therapy of endometriosis: transductional and transcriptional targeting of adenoviral vectors to endometriosis cells. American Journal of Obstetrics and Gynecology, 199(2), 117.e1-117.e6. https://doi.org/10.1016/j.ajog.2008.01.059

Toward gene therapy of endometriosis : transductional and transcriptional targeting of adenoviral vectors to endometriosis cells. / Othman, Essam Eldin R.; Zhu, Zeng B.; Curiel, David T.; Khatoon, Nilufar; Salem, Hosam T.; Khalifa, Essam Al Din M.; Al-Hendy, Ayman.

In: American Journal of Obstetrics and Gynecology, Vol. 199, No. 2, 01.01.2008, p. 117.e1-117.e6.

Research output: Contribution to journalArticle

Othman, Essam Eldin R. ; Zhu, Zeng B. ; Curiel, David T. ; Khatoon, Nilufar ; Salem, Hosam T. ; Khalifa, Essam Al Din M. ; Al-Hendy, Ayman. / Toward gene therapy of endometriosis : transductional and transcriptional targeting of adenoviral vectors to endometriosis cells. In: American Journal of Obstetrics and Gynecology. 2008 ; Vol. 199, No. 2. pp. 117.e1-117.e6.
@article{09f99983ed4c43faa0ab9d403824e040,
title = "Toward gene therapy of endometriosis: transductional and transcriptional targeting of adenoviral vectors to endometriosis cells",
abstract = "Objective: The purpose of this study was to screen a panel of targeted adenoviruses as vectors for endometriosis gene therapy. Study Design: Endometriotic cells were obtained from subjects with ovarian endometriomas. Liver tissues were taken from donors during hepatic transplantation surgery. Human endometriotic cells and liver tissues were transfected by targeted adenoviruses expressing luciferase reporter gene. Luciferase activity that was mediated by each virus was expressed as a percentage of adenovirus serotype 5 (Ad5-CMV-luc) activity. The 2-tailed Studentt test was used to compare the adenovirus data. Results: In endometriotic cells, the adenovirus-RGD (Ad-RGD-luc), adenovirus under secretory leukocyte protease inhibitor promoter (Ad-SLPI-luc), and adenovirus under heparanase promoter (Ad-heparanase-luc) showed significantly higher activity, compared with the adenovirus serotype 5. In liver tissues, adenovirus-survivin (Ad-survivin-luc) and Ad-heparanase-luc had significantly lower activity, compared with adenovirus serotype 5. Conclusion: Ad-heparanase-luc showed {"}endometriosis on, liver off{"} phenotype and is a promising vector for endometriosis gene therapy.",
keywords = "adenovirus, endometriosis, gene therapy, targeting strategy",
author = "Othman, {Essam Eldin R.} and Zhu, {Zeng B.} and Curiel, {David T.} and Nilufar Khatoon and Salem, {Hosam T.} and Khalifa, {Essam Al Din M.} and Ayman Al-Hendy",
year = "2008",
month = "1",
day = "1",
doi = "10.1016/j.ajog.2008.01.059",
language = "English (US)",
volume = "199",
pages = "117.e1--117.e6",
journal = "American Journal of Obstetrics and Gynecology",
issn = "0002-9378",
publisher = "Mosby Inc.",
number = "2",

}

TY - JOUR

T1 - Toward gene therapy of endometriosis

T2 - transductional and transcriptional targeting of adenoviral vectors to endometriosis cells

AU - Othman, Essam Eldin R.

AU - Zhu, Zeng B.

AU - Curiel, David T.

AU - Khatoon, Nilufar

AU - Salem, Hosam T.

AU - Khalifa, Essam Al Din M.

AU - Al-Hendy, Ayman

PY - 2008/1/1

Y1 - 2008/1/1

N2 - Objective: The purpose of this study was to screen a panel of targeted adenoviruses as vectors for endometriosis gene therapy. Study Design: Endometriotic cells were obtained from subjects with ovarian endometriomas. Liver tissues were taken from donors during hepatic transplantation surgery. Human endometriotic cells and liver tissues were transfected by targeted adenoviruses expressing luciferase reporter gene. Luciferase activity that was mediated by each virus was expressed as a percentage of adenovirus serotype 5 (Ad5-CMV-luc) activity. The 2-tailed Studentt test was used to compare the adenovirus data. Results: In endometriotic cells, the adenovirus-RGD (Ad-RGD-luc), adenovirus under secretory leukocyte protease inhibitor promoter (Ad-SLPI-luc), and adenovirus under heparanase promoter (Ad-heparanase-luc) showed significantly higher activity, compared with the adenovirus serotype 5. In liver tissues, adenovirus-survivin (Ad-survivin-luc) and Ad-heparanase-luc had significantly lower activity, compared with adenovirus serotype 5. Conclusion: Ad-heparanase-luc showed "endometriosis on, liver off" phenotype and is a promising vector for endometriosis gene therapy.

AB - Objective: The purpose of this study was to screen a panel of targeted adenoviruses as vectors for endometriosis gene therapy. Study Design: Endometriotic cells were obtained from subjects with ovarian endometriomas. Liver tissues were taken from donors during hepatic transplantation surgery. Human endometriotic cells and liver tissues were transfected by targeted adenoviruses expressing luciferase reporter gene. Luciferase activity that was mediated by each virus was expressed as a percentage of adenovirus serotype 5 (Ad5-CMV-luc) activity. The 2-tailed Studentt test was used to compare the adenovirus data. Results: In endometriotic cells, the adenovirus-RGD (Ad-RGD-luc), adenovirus under secretory leukocyte protease inhibitor promoter (Ad-SLPI-luc), and adenovirus under heparanase promoter (Ad-heparanase-luc) showed significantly higher activity, compared with the adenovirus serotype 5. In liver tissues, adenovirus-survivin (Ad-survivin-luc) and Ad-heparanase-luc had significantly lower activity, compared with adenovirus serotype 5. Conclusion: Ad-heparanase-luc showed "endometriosis on, liver off" phenotype and is a promising vector for endometriosis gene therapy.

KW - adenovirus

KW - endometriosis

KW - gene therapy

KW - targeting strategy

UR - http://www.scopus.com/inward/record.url?scp=47949092846&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=47949092846&partnerID=8YFLogxK

U2 - 10.1016/j.ajog.2008.01.059

DO - 10.1016/j.ajog.2008.01.059

M3 - Article

C2 - 18674655

AN - SCOPUS:47949092846

VL - 199

SP - 117.e1-117.e6

JO - American Journal of Obstetrics and Gynecology

JF - American Journal of Obstetrics and Gynecology

SN - 0002-9378

IS - 2

ER -