Toward using stable spermatozoal RNAs for prognostic assessment of male factor fertility

G. Charles Ostermeier, Robert J. Goodrich, Michael Peter Diamond, David J. Dix, Stephen A. Krawetz

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

Objective: To establish the stability of spermatozoal RNAs as a means to validate their use as a male fertility marker. Design: Semen samples were randomly selected for 1 of 3 cryopreservation treatments. Setting: An academic research environment. Patient(s): Men aged 19 to 55 years who had fathered a child by natural conception within the past 6 months. Intervention(s): Ejaculates were collected by masturbation and total spermatozoan RNA was isolated from two semen samples of ideal quality; one sample of medium quality, having been subjected to an additional freeze-thaw cycle, and two samples of poor quality, having been subjected to a third freeze-thaw cycle. Main Outcome Measure(s): Labeled cDNAs were generated and then used to interrogate Atlas Nylon Human Toxicology 1.2 microarrays. The spermatozoan transcriptomes were compared using a binomial approach. Result(s): The analysis identified a total of 228 unique spermatozoal transcripts among all samples. The medium quality sample shared 98% and 39% of its RNAs with the ideal and poor quality samples, respectively. A set of 36 RNAs resistant to insult were observed, some of which have been implicated in regulating male fertility, when all individuals were compared. Conclusion(s): These results support the view that a population of spermatozoal RNAs is rapidly degraded in response to insult, whereas another population appears protected from such damage. Because spermatozoal RNAs echo the gene expression of spermatogenesis, the latter is likely to prove useful as a clinical maker of fertility status.

Original languageEnglish (US)
Pages (from-to)1687-1694
Number of pages8
JournalFertility and sterility
Volume83
Issue number6
DOIs
StatePublished - Jun 1 2005

Fingerprint

Fertility
RNA
Semen
Masturbation
Atlases
Cryopreservation
Nylons
RNA Stability
Spermatogenesis
Transcriptome
Toxicology
Population
Complementary DNA
Outcome Assessment (Health Care)
Gene Expression
Research
Therapeutics

Keywords

  • Cryopreservation
  • Diagnosis
  • Microarray
  • RNA
  • Sperm transcripts

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

Cite this

Toward using stable spermatozoal RNAs for prognostic assessment of male factor fertility. / Ostermeier, G. Charles; Goodrich, Robert J.; Diamond, Michael Peter; Dix, David J.; Krawetz, Stephen A.

In: Fertility and sterility, Vol. 83, No. 6, 01.06.2005, p. 1687-1694.

Research output: Contribution to journalArticle

Ostermeier, G. Charles ; Goodrich, Robert J. ; Diamond, Michael Peter ; Dix, David J. ; Krawetz, Stephen A. / Toward using stable spermatozoal RNAs for prognostic assessment of male factor fertility. In: Fertility and sterility. 2005 ; Vol. 83, No. 6. pp. 1687-1694.
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