Toxic components of Auricularia polytricha

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

To find biologically active components of the higher fungi of Korea, the carpophores of Auricularia polytricha, a well-known edible mushroom, were extracted with 0.14 M NaCl solution. The extract was successively fractionated by adding ammonium sulfate at various concentrations, and the respective precipitates were separated by centrifugation, then dialyzed and freeze-dried. When a dose of 60 mg/kg of each was injected i.p. into ICR mice, the fraction which precipitated at 20% ammonium sulfate showed the highest toxicity, killing seven out of seven mice within two days. The fraction obtained at 40% ammonium sulfate showed the second highest toxicity. The two fractions were named auritoxin I and II after the genus name. However, they Nere shown to have nearly identical composition by physicochemical and instrumental analysis. The chemical analysis of auritoxin showed 93.9% polysac-charide and 6.8% protein. The polysaccharide moiety was found to have 12.3% α-linkage and 87.7% β-linkage and to be a heteromannoglucan consisting of 45.1% glucose, 43.9% mannose and 11.0% xylose. The protein moiety contained ten amino acids. The molecular weight of the toxin was 1.5×106 dalton by Sepharose CL-4B gel filtration. The median lethal doses of auritoxin in mice were 56.4, 157.2 and 454.6 mg/kg by i.p., s.c. and p.o. administrations, respectively. The signs of intoxication were convulsion during the first 30 minutes after the injection, coma or sleeping within an hour, tremor, lacrimation, nasal bleeding, congestion, and death in 24 hours. Among the various organs, the spleen was found to be enlarged remarkably. Human platelet aggregation was inhibited by the addition of auritoxin. The activity of malic dehydrogenase in vitro was inhibited by the toxin.

Original languageEnglish (US)
Pages (from-to)36-42
Number of pages7
JournalArchives of Pharmacal Research
Volume16
Issue number1
DOIs
StatePublished - Jan 1 1993
Externally publishedYes

Fingerprint

Poisons
Ammonium Sulfate
Toxicity
Inbred ICR Mouse
Malate Dehydrogenase
Epistaxis
Centrifugation
Agaricales
Xylose
Tremor
Korea
Mannose
Coma
Platelets
Fungi
Chemical analysis
Platelet Aggregation
Names
Gel Chromatography
Polysaccharides

Keywords

  • Auricularia polytricha
  • Auritoxin
  • Glycoprotein
  • Malic dehydrogenase
  • Median lethal dose
  • Platelet aggregation
  • Polysaccharide
  • Toxicity

ASJC Scopus subject areas

  • Molecular Medicine
  • Drug Discovery
  • Organic Chemistry

Cite this

Toxic components of Auricularia polytricha. / Kim, Ha Won.

In: Archives of Pharmacal Research, Vol. 16, No. 1, 01.01.1993, p. 36-42.

Research output: Contribution to journalArticle

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abstract = "To find biologically active components of the higher fungi of Korea, the carpophores of Auricularia polytricha, a well-known edible mushroom, were extracted with 0.14 M NaCl solution. The extract was successively fractionated by adding ammonium sulfate at various concentrations, and the respective precipitates were separated by centrifugation, then dialyzed and freeze-dried. When a dose of 60 mg/kg of each was injected i.p. into ICR mice, the fraction which precipitated at 20{\%} ammonium sulfate showed the highest toxicity, killing seven out of seven mice within two days. The fraction obtained at 40{\%} ammonium sulfate showed the second highest toxicity. The two fractions were named auritoxin I and II after the genus name. However, they Nere shown to have nearly identical composition by physicochemical and instrumental analysis. The chemical analysis of auritoxin showed 93.9{\%} polysac-charide and 6.8{\%} protein. The polysaccharide moiety was found to have 12.3{\%} α-linkage and 87.7{\%} β-linkage and to be a heteromannoglucan consisting of 45.1{\%} glucose, 43.9{\%} mannose and 11.0{\%} xylose. The protein moiety contained ten amino acids. The molecular weight of the toxin was 1.5×106 dalton by Sepharose CL-4B gel filtration. The median lethal doses of auritoxin in mice were 56.4, 157.2 and 454.6 mg/kg by i.p., s.c. and p.o. administrations, respectively. The signs of intoxication were convulsion during the first 30 minutes after the injection, coma or sleeping within an hour, tremor, lacrimation, nasal bleeding, congestion, and death in 24 hours. Among the various organs, the spleen was found to be enlarged remarkably. Human platelet aggregation was inhibited by the addition of auritoxin. The activity of malic dehydrogenase in vitro was inhibited by the toxin.",
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N2 - To find biologically active components of the higher fungi of Korea, the carpophores of Auricularia polytricha, a well-known edible mushroom, were extracted with 0.14 M NaCl solution. The extract was successively fractionated by adding ammonium sulfate at various concentrations, and the respective precipitates were separated by centrifugation, then dialyzed and freeze-dried. When a dose of 60 mg/kg of each was injected i.p. into ICR mice, the fraction which precipitated at 20% ammonium sulfate showed the highest toxicity, killing seven out of seven mice within two days. The fraction obtained at 40% ammonium sulfate showed the second highest toxicity. The two fractions were named auritoxin I and II after the genus name. However, they Nere shown to have nearly identical composition by physicochemical and instrumental analysis. The chemical analysis of auritoxin showed 93.9% polysac-charide and 6.8% protein. The polysaccharide moiety was found to have 12.3% α-linkage and 87.7% β-linkage and to be a heteromannoglucan consisting of 45.1% glucose, 43.9% mannose and 11.0% xylose. The protein moiety contained ten amino acids. The molecular weight of the toxin was 1.5×106 dalton by Sepharose CL-4B gel filtration. The median lethal doses of auritoxin in mice were 56.4, 157.2 and 454.6 mg/kg by i.p., s.c. and p.o. administrations, respectively. The signs of intoxication were convulsion during the first 30 minutes after the injection, coma or sleeping within an hour, tremor, lacrimation, nasal bleeding, congestion, and death in 24 hours. Among the various organs, the spleen was found to be enlarged remarkably. Human platelet aggregation was inhibited by the addition of auritoxin. The activity of malic dehydrogenase in vitro was inhibited by the toxin.

AB - To find biologically active components of the higher fungi of Korea, the carpophores of Auricularia polytricha, a well-known edible mushroom, were extracted with 0.14 M NaCl solution. The extract was successively fractionated by adding ammonium sulfate at various concentrations, and the respective precipitates were separated by centrifugation, then dialyzed and freeze-dried. When a dose of 60 mg/kg of each was injected i.p. into ICR mice, the fraction which precipitated at 20% ammonium sulfate showed the highest toxicity, killing seven out of seven mice within two days. The fraction obtained at 40% ammonium sulfate showed the second highest toxicity. The two fractions were named auritoxin I and II after the genus name. However, they Nere shown to have nearly identical composition by physicochemical and instrumental analysis. The chemical analysis of auritoxin showed 93.9% polysac-charide and 6.8% protein. The polysaccharide moiety was found to have 12.3% α-linkage and 87.7% β-linkage and to be a heteromannoglucan consisting of 45.1% glucose, 43.9% mannose and 11.0% xylose. The protein moiety contained ten amino acids. The molecular weight of the toxin was 1.5×106 dalton by Sepharose CL-4B gel filtration. The median lethal doses of auritoxin in mice were 56.4, 157.2 and 454.6 mg/kg by i.p., s.c. and p.o. administrations, respectively. The signs of intoxication were convulsion during the first 30 minutes after the injection, coma or sleeping within an hour, tremor, lacrimation, nasal bleeding, congestion, and death in 24 hours. Among the various organs, the spleen was found to be enlarged remarkably. Human platelet aggregation was inhibited by the addition of auritoxin. The activity of malic dehydrogenase in vitro was inhibited by the toxin.

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