Toxic neurofilamentous axonopathies and fast axonal transport. v. reduced bidirectional vesicle transport in cultured neurons by acrylamide and glycidamide

C. H. Harris, Adarsh K Gulati, M. A. Friedman, D. W. Sickles

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Fast axonal transport deficiencies as mechanisms of action of aoylamide in producing axonal degeneration are under evaluation. The current study determines the effects of acry- lamide and several analogues on the number of vesicles moving within the neurite processes of cultured rat embryonic neurons. Acrylamide produced severe, concentration- dependent (0.25-1.0 mM) and time-dependent (0-60 min) reduction in the quantity of vesicles translocated in both the anterograde and retrograde directions. Clycidamide, a potential neurotoxic metabolite of acrylamide, produced a time-dependent but not a concentration-dependent (in the 0.25-1.0 mM range) reduction in bidirectional transport. Based on inhibition at 60 min, glycidamide was estimated to be 4 times more potent than acrylamide in altering transport. Propionamide, a C1,-C2saturated nonneurotoxic acrylamide analogue, had no effect on axonal transport. While a tendency for methylene bisacrylamide (MbACR) to reduce vesicle transport was noted, at the concentration used no statistically significant differences from control were observed. The data support the correlation between toxicant-induced fast anterograde and retrograde axonal transport reductions and axonal degeneration produced by acrylamide and its analogues.

Original languageEnglish (US)
Pages (from-to)343-356
Number of pages14
JournalJournal of Toxicology and Environmental Health
Volume42
Issue number3
DOIs
StatePublished - Jan 1 1994

Fingerprint

Transport Vesicles
Axonal Transport
Acrylamide
Poisons
Neurons
Metabolites
Rats
Neurites
glycidamide

ASJC Scopus subject areas

  • Toxicology
  • Pollution

Cite this

Toxic neurofilamentous axonopathies and fast axonal transport. v. reduced bidirectional vesicle transport in cultured neurons by acrylamide and glycidamide. / Harris, C. H.; Gulati, Adarsh K; Friedman, M. A.; Sickles, D. W.

In: Journal of Toxicology and Environmental Health, Vol. 42, No. 3, 01.01.1994, p. 343-356.

Research output: Contribution to journalArticle

@article{5892fc6ef6b64b36a89dfe296ff9fb92,
title = "Toxic neurofilamentous axonopathies and fast axonal transport. v. reduced bidirectional vesicle transport in cultured neurons by acrylamide and glycidamide",
abstract = "Fast axonal transport deficiencies as mechanisms of action of aoylamide in producing axonal degeneration are under evaluation. The current study determines the effects of acry- lamide and several analogues on the number of vesicles moving within the neurite processes of cultured rat embryonic neurons. Acrylamide produced severe, concentration- dependent (0.25-1.0 mM) and time-dependent (0-60 min) reduction in the quantity of vesicles translocated in both the anterograde and retrograde directions. Clycidamide, a potential neurotoxic metabolite of acrylamide, produced a time-dependent but not a concentration-dependent (in the 0.25-1.0 mM range) reduction in bidirectional transport. Based on inhibition at 60 min, glycidamide was estimated to be 4 times more potent than acrylamide in altering transport. Propionamide, a C1,-C2saturated nonneurotoxic acrylamide analogue, had no effect on axonal transport. While a tendency for methylene bisacrylamide (MbACR) to reduce vesicle transport was noted, at the concentration used no statistically significant differences from control were observed. The data support the correlation between toxicant-induced fast anterograde and retrograde axonal transport reductions and axonal degeneration produced by acrylamide and its analogues.",
author = "Harris, {C. H.} and Gulati, {Adarsh K} and Friedman, {M. A.} and Sickles, {D. W.}",
year = "1994",
month = "1",
day = "1",
doi = "10.1080/15287399409531884",
language = "English (US)",
volume = "42",
pages = "343--356",
journal = "Journal of Toxicology and Environmental Health",
issn = "0098-4108",
publisher = "Taylor and Francis Ltd.",
number = "3",

}

TY - JOUR

T1 - Toxic neurofilamentous axonopathies and fast axonal transport. v. reduced bidirectional vesicle transport in cultured neurons by acrylamide and glycidamide

AU - Harris, C. H.

AU - Gulati, Adarsh K

AU - Friedman, M. A.

AU - Sickles, D. W.

PY - 1994/1/1

Y1 - 1994/1/1

N2 - Fast axonal transport deficiencies as mechanisms of action of aoylamide in producing axonal degeneration are under evaluation. The current study determines the effects of acry- lamide and several analogues on the number of vesicles moving within the neurite processes of cultured rat embryonic neurons. Acrylamide produced severe, concentration- dependent (0.25-1.0 mM) and time-dependent (0-60 min) reduction in the quantity of vesicles translocated in both the anterograde and retrograde directions. Clycidamide, a potential neurotoxic metabolite of acrylamide, produced a time-dependent but not a concentration-dependent (in the 0.25-1.0 mM range) reduction in bidirectional transport. Based on inhibition at 60 min, glycidamide was estimated to be 4 times more potent than acrylamide in altering transport. Propionamide, a C1,-C2saturated nonneurotoxic acrylamide analogue, had no effect on axonal transport. While a tendency for methylene bisacrylamide (MbACR) to reduce vesicle transport was noted, at the concentration used no statistically significant differences from control were observed. The data support the correlation between toxicant-induced fast anterograde and retrograde axonal transport reductions and axonal degeneration produced by acrylamide and its analogues.

AB - Fast axonal transport deficiencies as mechanisms of action of aoylamide in producing axonal degeneration are under evaluation. The current study determines the effects of acry- lamide and several analogues on the number of vesicles moving within the neurite processes of cultured rat embryonic neurons. Acrylamide produced severe, concentration- dependent (0.25-1.0 mM) and time-dependent (0-60 min) reduction in the quantity of vesicles translocated in both the anterograde and retrograde directions. Clycidamide, a potential neurotoxic metabolite of acrylamide, produced a time-dependent but not a concentration-dependent (in the 0.25-1.0 mM range) reduction in bidirectional transport. Based on inhibition at 60 min, glycidamide was estimated to be 4 times more potent than acrylamide in altering transport. Propionamide, a C1,-C2saturated nonneurotoxic acrylamide analogue, had no effect on axonal transport. While a tendency for methylene bisacrylamide (MbACR) to reduce vesicle transport was noted, at the concentration used no statistically significant differences from control were observed. The data support the correlation between toxicant-induced fast anterograde and retrograde axonal transport reductions and axonal degeneration produced by acrylamide and its analogues.

UR - http://www.scopus.com/inward/record.url?scp=0028175589&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028175589&partnerID=8YFLogxK

U2 - 10.1080/15287399409531884

DO - 10.1080/15287399409531884

M3 - Article

C2 - 7517455

AN - SCOPUS:0028175589

VL - 42

SP - 343

EP - 356

JO - Journal of Toxicology and Environmental Health

JF - Journal of Toxicology and Environmental Health

SN - 0098-4108

IS - 3

ER -