Toxicity effect of silver nanoparticles on photosynthetic pigment content, growth, ROS production and ultrastructural changes of microalgae chlorella vulgaris

Layla J. Hazeem; Gamze Kuku; Etienne Dewailly; Christian Slomianny; Alexandre Barras; Abderrahmane Hamdi; Rabah Boukherroub; Mustafa Culha; Mohamed Bououdina

doi:10.3390/nano9070914

Toxicity effect of silver nanoparticles on photosynthetic pigment content, growth, ROS production and ultrastructural changes of microalgae chlorella vulgaris

Layla J. Hazeem, Gamze Kuku, Etienne Dewailly, Christian Slomianny, Alexandre Barras, Abderrahmane Hamdi, Rabah Boukherroub, Mustafa Culha, Mohamed Bououdina

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

Silver nanoparticles (Ag NPs) exhibit antibacterial activity and are extensively used in numerous applications. The aim of this study was to examine the toxic effect of Ag NPs on the marine microalga, Chlorella vulgaris. The microalgae, at the exponential growth phase, were treated with different concentrations of Ag NPs (50 and 100 nm) for 96 h. X-Ray diffraction (XRD) results indicated that the used NPs are single and pure Ag phase with a mean crystallite size of 21 and 32 nm. Ag NPs were found to have a negative effect on viable cell concentration, a variable effect on chlorophyll a concentration, and increased ROS formation. Transmission electron microscopy (TEM) analysis revealed that Ag NPs were present inside the microalgae cells and formed large aggregates in the culture medium. Ag⁺ ions, in the form of AgNO₃, were also assessed at higher concentrations and found to cause inhibitory effects.

Original language	English (US)
Article number	914
Journal	Nanomaterials
Volume	9
Issue number	7
DOIs	https://doi.org/10.3390/nano9070914
State	Published - Jul 2019
Externally published	Yes

Keywords

Ag ions
Chlorella vulgaris
Chlorophyll a
Reactive oxygen species
Silver nanoparticles
Viable cells

ASJC Scopus subject areas

General Chemical Engineering
General Materials Science

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/nano9070914

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title = "Toxicity effect of silver nanoparticles on photosynthetic pigment content, growth, ROS production and ultrastructural changes of microalgae chlorella vulgaris",

abstract = "Silver nanoparticles (Ag NPs) exhibit antibacterial activity and are extensively used in numerous applications. The aim of this study was to examine the toxic effect of Ag NPs on the marine microalga, Chlorella vulgaris. The microalgae, at the exponential growth phase, were treated with different concentrations of Ag NPs (50 and 100 nm) for 96 h. X-Ray diffraction (XRD) results indicated that the used NPs are single and pure Ag phase with a mean crystallite size of 21 and 32 nm. Ag NPs were found to have a negative effect on viable cell concentration, a variable effect on chlorophyll a concentration, and increased ROS formation. Transmission electron microscopy (TEM) analysis revealed that Ag NPs were present inside the microalgae cells and formed large aggregates in the culture medium. Ag+ ions, in the form of AgNO3, were also assessed at higher concentrations and found to cause inhibitory effects.",

keywords = "Ag ions, Chlorella vulgaris, Chlorophyll a, Reactive oxygen species, Silver nanoparticles, Viable cells",

author = "Hazeem, {Layla J.} and Gamze Kuku and Etienne Dewailly and Christian Slomianny and Alexandre Barras and Abderrahmane Hamdi and Rabah Boukherroub and Mustafa Culha and Mohamed Bououdina",

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year = "2019",

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doi = "10.3390/nano9070914",

language = "English (US)",

volume = "9",

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AU - Hazeem, Layla J.

AU - Kuku, Gamze

AU - Dewailly, Etienne

AU - Slomianny, Christian

AU - Barras, Alexandre

AU - Hamdi, Abderrahmane

AU - Boukherroub, Rabah

AU - Culha, Mustafa

AU - Bououdina, Mohamed

PY - 2019/7

Y1 - 2019/7

N2 - Silver nanoparticles (Ag NPs) exhibit antibacterial activity and are extensively used in numerous applications. The aim of this study was to examine the toxic effect of Ag NPs on the marine microalga, Chlorella vulgaris. The microalgae, at the exponential growth phase, were treated with different concentrations of Ag NPs (50 and 100 nm) for 96 h. X-Ray diffraction (XRD) results indicated that the used NPs are single and pure Ag phase with a mean crystallite size of 21 and 32 nm. Ag NPs were found to have a negative effect on viable cell concentration, a variable effect on chlorophyll a concentration, and increased ROS formation. Transmission electron microscopy (TEM) analysis revealed that Ag NPs were present inside the microalgae cells and formed large aggregates in the culture medium. Ag+ ions, in the form of AgNO3, were also assessed at higher concentrations and found to cause inhibitory effects.

AB - Silver nanoparticles (Ag NPs) exhibit antibacterial activity and are extensively used in numerous applications. The aim of this study was to examine the toxic effect of Ag NPs on the marine microalga, Chlorella vulgaris. The microalgae, at the exponential growth phase, were treated with different concentrations of Ag NPs (50 and 100 nm) for 96 h. X-Ray diffraction (XRD) results indicated that the used NPs are single and pure Ag phase with a mean crystallite size of 21 and 32 nm. Ag NPs were found to have a negative effect on viable cell concentration, a variable effect on chlorophyll a concentration, and increased ROS formation. Transmission electron microscopy (TEM) analysis revealed that Ag NPs were present inside the microalgae cells and formed large aggregates in the culture medium. Ag+ ions, in the form of AgNO3, were also assessed at higher concentrations and found to cause inhibitory effects.

KW - Ag ions

KW - Chlorella vulgaris

KW - Chlorophyll a

KW - Reactive oxygen species

KW - Silver nanoparticles

KW - Viable cells

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Toxicity effect of silver nanoparticles on photosynthetic pigment content, growth, ROS production and ultrastructural changes of microalgae chlorella vulgaris

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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