Transcriptional profiling of uterine leiomyoma rats treated by a traditional herb pair, curcumae rhizoma and sparganii rhizoma

Cheng Hao Yu, Jin Shuang Zhao, Hui Zhao, Teng Peng, Dong Cheng Shen, Qiu Xia Xu, Yao Li, R Clinton Webb, Mong Heng Wang, Xing Ming Shi, Cheng Peng, Wei Jun Ding

Research output: Contribution to journalArticle

Abstract

The aim of this study was to elucidate the concise effects of a traditional herb pair, Curcumae rhizoma-Sparganii rhizoma (CRSR), on uterine leiomyoma (UL) by analyzing transcriptional profiling. The UL rat model was made by intramuscular injection of progesterone and gavage administration of diethylstilbestrol. From 11 weeks of the establishment of the model, rats of the UL+CRSR group were gavaged daily with CRSR (6.67 g/kg). The serum concentrations of progesterone (P) and estradiol (E2) were determined by radioimmunoassay, the uterine index was measured by caliper measurement, and the pathological status was observed by hematoxylin and eosin stain. Gene expression profiling was checked by NimbleGen Rat Gene Expression Microarrays. The results indicated that the uterine mass of UL+CRSR rats was significantly shrunk and serum P and E2 levels significantly reduced compared to UL animals and nearly to the level of normal rats. Results of microarrays displayed the extensive inhibition of CRSR upon the expression of proliferation and deposition of extracellular matrix (ECM)-related genes, and significantly regulated a wide range of metabolism disorders. Furthermore, CRSR extensively regulated key pathways of the UL process, such as MAPK, PPAR, Notch, and TGF-b/Smad. Regulation of the crucial pathways for the UL process and ECM metabolism may be the underlying mechanisms of CRSR treatment. Further studies will provide clear clues for effectively treating UL with CRSR.

Original languageEnglish (US)
Article numbere8132
JournalBrazilian Journal of Medical and Biological Research
Volume52
Issue number6
DOIs
StatePublished - Jan 1 2019

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Leiomyoma
Rats
Microarrays
Metabolism
Gene expression
Progesterone
Peroxisome Proliferator-Activated Receptors
Diethylstilbestrol
Extracellular Matrix
Hematoxylin
Eosine Yellowish-(YS)
Estradiol
Animals
Coloring Agents
Genes
Intramuscular Injections
Gene Expression Profiling
Serum
Radioimmunoassay
Gene Expression

Keywords

  • Curcumae rhizoma
  • Extracellular matrix
  • Sparganii rhizoma
  • Transcriptional profiling
  • Uterine leiomyoma

ASJC Scopus subject areas

  • Biophysics
  • Neuroscience(all)
  • Biochemistry
  • Physiology
  • Immunology
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Cell Biology

Cite this

Transcriptional profiling of uterine leiomyoma rats treated by a traditional herb pair, curcumae rhizoma and sparganii rhizoma. / Yu, Cheng Hao; Zhao, Jin Shuang; Zhao, Hui; Peng, Teng; Shen, Dong Cheng; Xu, Qiu Xia; Li, Yao; Webb, R Clinton; Wang, Mong Heng; Shi, Xing Ming; Peng, Cheng; Ding, Wei Jun.

In: Brazilian Journal of Medical and Biological Research, Vol. 52, No. 6, e8132, 01.01.2019.

Research output: Contribution to journalArticle

Yu, Cheng Hao ; Zhao, Jin Shuang ; Zhao, Hui ; Peng, Teng ; Shen, Dong Cheng ; Xu, Qiu Xia ; Li, Yao ; Webb, R Clinton ; Wang, Mong Heng ; Shi, Xing Ming ; Peng, Cheng ; Ding, Wei Jun. / Transcriptional profiling of uterine leiomyoma rats treated by a traditional herb pair, curcumae rhizoma and sparganii rhizoma. In: Brazilian Journal of Medical and Biological Research. 2019 ; Vol. 52, No. 6.
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abstract = "The aim of this study was to elucidate the concise effects of a traditional herb pair, Curcumae rhizoma-Sparganii rhizoma (CRSR), on uterine leiomyoma (UL) by analyzing transcriptional profiling. The UL rat model was made by intramuscular injection of progesterone and gavage administration of diethylstilbestrol. From 11 weeks of the establishment of the model, rats of the UL+CRSR group were gavaged daily with CRSR (6.67 g/kg). The serum concentrations of progesterone (P) and estradiol (E2) were determined by radioimmunoassay, the uterine index was measured by caliper measurement, and the pathological status was observed by hematoxylin and eosin stain. Gene expression profiling was checked by NimbleGen Rat Gene Expression Microarrays. The results indicated that the uterine mass of UL+CRSR rats was significantly shrunk and serum P and E2 levels significantly reduced compared to UL animals and nearly to the level of normal rats. Results of microarrays displayed the extensive inhibition of CRSR upon the expression of proliferation and deposition of extracellular matrix (ECM)-related genes, and significantly regulated a wide range of metabolism disorders. Furthermore, CRSR extensively regulated key pathways of the UL process, such as MAPK, PPAR, Notch, and TGF-b/Smad. Regulation of the crucial pathways for the UL process and ECM metabolism may be the underlying mechanisms of CRSR treatment. Further studies will provide clear clues for effectively treating UL with CRSR.",
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AU - Yu, Cheng Hao

AU - Zhao, Jin Shuang

AU - Zhao, Hui

AU - Peng, Teng

AU - Shen, Dong Cheng

AU - Xu, Qiu Xia

AU - Li, Yao

AU - Webb, R Clinton

AU - Wang, Mong Heng

AU - Shi, Xing Ming

AU - Peng, Cheng

AU - Ding, Wei Jun

PY - 2019/1/1

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N2 - The aim of this study was to elucidate the concise effects of a traditional herb pair, Curcumae rhizoma-Sparganii rhizoma (CRSR), on uterine leiomyoma (UL) by analyzing transcriptional profiling. The UL rat model was made by intramuscular injection of progesterone and gavage administration of diethylstilbestrol. From 11 weeks of the establishment of the model, rats of the UL+CRSR group were gavaged daily with CRSR (6.67 g/kg). The serum concentrations of progesterone (P) and estradiol (E2) were determined by radioimmunoassay, the uterine index was measured by caliper measurement, and the pathological status was observed by hematoxylin and eosin stain. Gene expression profiling was checked by NimbleGen Rat Gene Expression Microarrays. The results indicated that the uterine mass of UL+CRSR rats was significantly shrunk and serum P and E2 levels significantly reduced compared to UL animals and nearly to the level of normal rats. Results of microarrays displayed the extensive inhibition of CRSR upon the expression of proliferation and deposition of extracellular matrix (ECM)-related genes, and significantly regulated a wide range of metabolism disorders. Furthermore, CRSR extensively regulated key pathways of the UL process, such as MAPK, PPAR, Notch, and TGF-b/Smad. Regulation of the crucial pathways for the UL process and ECM metabolism may be the underlying mechanisms of CRSR treatment. Further studies will provide clear clues for effectively treating UL with CRSR.

AB - The aim of this study was to elucidate the concise effects of a traditional herb pair, Curcumae rhizoma-Sparganii rhizoma (CRSR), on uterine leiomyoma (UL) by analyzing transcriptional profiling. The UL rat model was made by intramuscular injection of progesterone and gavage administration of diethylstilbestrol. From 11 weeks of the establishment of the model, rats of the UL+CRSR group were gavaged daily with CRSR (6.67 g/kg). The serum concentrations of progesterone (P) and estradiol (E2) were determined by radioimmunoassay, the uterine index was measured by caliper measurement, and the pathological status was observed by hematoxylin and eosin stain. Gene expression profiling was checked by NimbleGen Rat Gene Expression Microarrays. The results indicated that the uterine mass of UL+CRSR rats was significantly shrunk and serum P and E2 levels significantly reduced compared to UL animals and nearly to the level of normal rats. Results of microarrays displayed the extensive inhibition of CRSR upon the expression of proliferation and deposition of extracellular matrix (ECM)-related genes, and significantly regulated a wide range of metabolism disorders. Furthermore, CRSR extensively regulated key pathways of the UL process, such as MAPK, PPAR, Notch, and TGF-b/Smad. Regulation of the crucial pathways for the UL process and ECM metabolism may be the underlying mechanisms of CRSR treatment. Further studies will provide clear clues for effectively treating UL with CRSR.

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