Transforming growth factor-β1 modulates expression of adhesion and cytoskeletal proteins in human peritoneal fibroblasts

Ujjwal K. Rout, Ghassan M. Saed, Michael P. Diamond

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

Objective: To determine the effects of TGF-β1 on the expression of the α1, α2, α5, αv, and α6 integrin subunits and on vinculin, and F-actin in human peritoneal fibroblasts. Design: Descriptive study using cell culture, reverse transcriptase-polymerase chain reaction (RT-PCR), and immunofluorescent and confocal microscopy. Setting: Academic medical center. Patient(s): Gynecological surgery patients. Intervention(s): None. Main Outcome Measure(s): Effects of TGF-β1 on the steady state levels of α5, αv, and α6 integrin transcripts were examined in the normal peritoneal fibroblasts using RT-PCR. Expression levels of the α1, α2, α5, αv, and α6 integrin subunits and of F-actin were measured by immunofluorescent microscopy. The distribution pattern of the integrin subunits, vinculin, and F-actin were examined using confocal microscopy. Result(s): TGF-β1 significantly up-regulated the expression levels of the α5, αv, and α6 integrin subunits and modulated their expression pattern, resulting in relatively higher levels of these subunits in the focal contacts of peritoneal fibroblasts. It allocated vinculin expression primarily to the focal contacts of cells and caused distortion of F-actin structure. The transcript levels of the α5, αv, and α6 integrin subunits were not altered by TGF-β1 treatment. Conclusion(s): TGF-β1 may promote postoperative adhesion formation by inducing the migration of peritoneal fibroblasts by altering the expression levels and patterns of specific integrin subunits, vinculin, and F-actin.

Original languageEnglish (US)
Pages (from-to)154-161
Number of pages8
JournalFertility and sterility
Volume78
Issue number1
DOIs
StatePublished - Jul 15 2002
Externally publishedYes

Keywords

  • Adhesion
  • F-actin
  • Immunofluorescence
  • Integrin
  • PCR
  • Peritoneal fibroblasts
  • TGF-β
  • Vinculin

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

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