Transforming growth factor-B1 and matrix metalloproteinase-7 promoter variants induce risk for Helicobacter pylori-associated gastric precancerous lesions

B R Achyut, Uday C Ghoshal, Nikhil Moorchung, Balraj Mittal

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

The expression of growth factors, proteolytic enzymes, fibrogenic factors, and cytokines is altered in the Helicobacter pylori-infected gastric mucosa. Therefore, we aimed to evaluate the association of functional promoter variants of transforming growth factor (TGF)-B1 and matrix metalloproteinase (MMP)-7 genes with gastritis and gastric precancerous lesions. After upper gastrointestinal endoscopy, a total of 130 rapid urease test-positive patients with nonulcer dyspepsia were examined for H. pylori infection using modified Giemsa stain and IgG anti-CagA ELISA. All patients and 200 asymptomatic controls were genotyped for TGF-B1 (-509 C>T) and MMP-7 (-181 A>G) substitutions using PCR-RFLP. The genotype and allele frequencies of TGF-B1 and MMP-7 polymorphisms did not differ between patients and controls (p > 0.05). However, the CagA-positive patients with TGF-B1 -509 T allele had higher risk for gastric atrophy (p = 0.026, odds ratio [OR] = 2.38) and lymphoid follicle development (p = 0.028, OR = 2.29). In addition, CagA-positive patients carrying MMP-7 -181 G allele had risk for lymphoid follicle formation (p = 0.027, OR = 2.30). Thus, the present study revealed significant association of functional MMP-7 and TGF-B1 gene variants toward susceptibility to H. pylori-induced precancerous gastric lesions.

Original languageEnglish (US)
Pages (from-to)295-301
Number of pages7
JournalDNA and Cell Biology
Volume28
Issue number6
DOIs
StatePublished - Jun 2009
Externally publishedYes

Keywords

  • Adult
  • Alleles
  • Antigens, Bacterial
  • Atrophy
  • Bacterial Proteins
  • Female
  • Gastritis
  • Genotype
  • Helicobacter Infections
  • Helicobacter pylori
  • Humans
  • Lymphoid Tissue
  • Male
  • Matrix Metalloproteinase 7
  • Middle Aged
  • Polymorphism, Single Nucleotide
  • Precancerous Conditions
  • Promoter Regions, Genetic
  • Risk
  • Sequence Deletion
  • Stomach
  • Stomach Neoplasms
  • Transforming Growth Factor beta1
  • Journal Article
  • Research Support, Non-U.S. Gov't

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