Transforming growth factor-B1 and matrix metalloproteinase-7 promoter variants induce risk for Helicobacter pylori-associated gastric precancerous lesions

B R Achyut, Uday C Ghoshal, Nikhil Moorchung, Balraj Mittal

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The expression of growth factors, proteolytic enzymes, fibrogenic factors, and cytokines is altered in the Helicobacter pylori-infected gastric mucosa. Therefore, we aimed to evaluate the association of functional promoter variants of transforming growth factor (TGF)-B1 and matrix metalloproteinase (MMP)-7 genes with gastritis and gastric precancerous lesions. After upper gastrointestinal endoscopy, a total of 130 rapid urease test-positive patients with nonulcer dyspepsia were examined for H. pylori infection using modified Giemsa stain and IgG anti-CagA ELISA. All patients and 200 asymptomatic controls were genotyped for TGF-B1 (-509 C>T) and MMP-7 (-181 A>G) substitutions using PCR-RFLP. The genotype and allele frequencies of TGF-B1 and MMP-7 polymorphisms did not differ between patients and controls (p > 0.05). However, the CagA-positive patients with TGF-B1 -509 T allele had higher risk for gastric atrophy (p = 0.026, odds ratio [OR] = 2.38) and lymphoid follicle development (p = 0.028, OR = 2.29). In addition, CagA-positive patients carrying MMP-7 -181 G allele had risk for lymphoid follicle formation (p = 0.027, OR = 2.30). Thus, the present study revealed significant association of functional MMP-7 and TGF-B1 gene variants toward susceptibility to H. pylori-induced precancerous gastric lesions.

Original languageEnglish (US)
Pages (from-to)295-301
Number of pages7
JournalDNA and Cell Biology
Volume28
Issue number6
DOIs
StatePublished - Jun 2009
Externally publishedYes

Fingerprint

Matrix Metalloproteinase 7
Transforming Growth Factors
Helicobacter pylori
Stomach
Odds Ratio
Alleles
Azure Stains
Gastrointestinal Endoscopy
Urease
Dyspepsia
Helicobacter Infections
Gastritis
Gastric Mucosa
Gene Frequency
Restriction Fragment Length Polymorphisms
Genes
Atrophy
Intercellular Signaling Peptides and Proteins
Peptide Hydrolases
Enzyme-Linked Immunosorbent Assay

Keywords

  • Adult
  • Alleles
  • Antigens, Bacterial
  • Atrophy
  • Bacterial Proteins
  • Female
  • Gastritis
  • Genotype
  • Helicobacter Infections
  • Helicobacter pylori
  • Humans
  • Lymphoid Tissue
  • Male
  • Matrix Metalloproteinase 7
  • Middle Aged
  • Polymorphism, Single Nucleotide
  • Precancerous Conditions
  • Promoter Regions, Genetic
  • Risk
  • Sequence Deletion
  • Stomach
  • Stomach Neoplasms
  • Transforming Growth Factor beta1
  • Journal Article
  • Research Support, Non-U.S. Gov't

Cite this

Transforming growth factor-B1 and matrix metalloproteinase-7 promoter variants induce risk for Helicobacter pylori-associated gastric precancerous lesions. / Achyut, B R; Ghoshal, Uday C; Moorchung, Nikhil; Mittal, Balraj.

In: DNA and Cell Biology, Vol. 28, No. 6, 06.2009, p. 295-301.

Research output: Contribution to journalArticle

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AB - The expression of growth factors, proteolytic enzymes, fibrogenic factors, and cytokines is altered in the Helicobacter pylori-infected gastric mucosa. Therefore, we aimed to evaluate the association of functional promoter variants of transforming growth factor (TGF)-B1 and matrix metalloproteinase (MMP)-7 genes with gastritis and gastric precancerous lesions. After upper gastrointestinal endoscopy, a total of 130 rapid urease test-positive patients with nonulcer dyspepsia were examined for H. pylori infection using modified Giemsa stain and IgG anti-CagA ELISA. All patients and 200 asymptomatic controls were genotyped for TGF-B1 (-509 C>T) and MMP-7 (-181 A>G) substitutions using PCR-RFLP. The genotype and allele frequencies of TGF-B1 and MMP-7 polymorphisms did not differ between patients and controls (p > 0.05). However, the CagA-positive patients with TGF-B1 -509 T allele had higher risk for gastric atrophy (p = 0.026, odds ratio [OR] = 2.38) and lymphoid follicle development (p = 0.028, OR = 2.29). In addition, CagA-positive patients carrying MMP-7 -181 G allele had risk for lymphoid follicle formation (p = 0.027, OR = 2.30). Thus, the present study revealed significant association of functional MMP-7 and TGF-B1 gene variants toward susceptibility to H. pylori-induced precancerous gastric lesions.

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