TY - JOUR
T1 - Transfusion-induced immunosuppression results in diminished host survival in a murine neuroblastoma model
AU - Lieberman, Michael D.
AU - Shou, Jian
AU - Sigal, Robert K.
AU - Yu, Jack
AU - Goldfine, Jill
AU - Daly, John M.
N1 - Funding Information:
1 Supported in part by the Georgene S. Harmelin Surgical Oncology Research Fund and by NC1 Grant 5-T32-CA 09619-o. Dr. Lieberman is a recipient of the Cletus W. Schwegman Oncology Research Fellowship.
PY - 1990/5
Y1 - 1990/5
N2 - Perioperative blood transfusion has been associated with decreased survival in cancer patients. The immunologic consequences of H-2 incompatible blood transfusion as related to neoplasia are unclear. This report examined the effect of multiple allogeneic blood transfusions, compared to syngeneic transfusions and saline infusion, on cellular immunity, tumor growth, and host survival in a murine C1300 neuroblastoma model. A/J mice were randomized to receive two weekly transfusions of washed whole blood cells from C57 B1/6 or A/J donors or saline. Animals transfused with allogeneic blood, compared to syngeneic transfusions or saline infusions, had a significantly diminished lymphocyte response to mitogen (P < 0.001), reduced donor-specific (P < 0.001) and third party alloantigen (P < 0.01) MLR, and reduced cytotoxicity against a natural killer (NK) cell-sensitive target (P < 0.001). These in vitro deficits in cellular immunity correlated with a significantly greater Day 21 tumor weight to total body weight ratio in the allogeneic group (0.33) compared with the syngeneic (0.25) and saline (0.28) groups P < 0.05). Median host survival was reduced in the allogeneic group (24 days) compared with the syngeneic (30 days) and saline (31 days) groups. There were no significant differences in cellular immunity, tumor growth, or survival between syngeneic and saline control groups. Allogeneic blood transfusion had an adverse affect on NK and T-lymphocyte function which was associated with enhanced tumor growth and reduced survival in tumor-bearing mice.
AB - Perioperative blood transfusion has been associated with decreased survival in cancer patients. The immunologic consequences of H-2 incompatible blood transfusion as related to neoplasia are unclear. This report examined the effect of multiple allogeneic blood transfusions, compared to syngeneic transfusions and saline infusion, on cellular immunity, tumor growth, and host survival in a murine C1300 neuroblastoma model. A/J mice were randomized to receive two weekly transfusions of washed whole blood cells from C57 B1/6 or A/J donors or saline. Animals transfused with allogeneic blood, compared to syngeneic transfusions or saline infusions, had a significantly diminished lymphocyte response to mitogen (P < 0.001), reduced donor-specific (P < 0.001) and third party alloantigen (P < 0.01) MLR, and reduced cytotoxicity against a natural killer (NK) cell-sensitive target (P < 0.001). These in vitro deficits in cellular immunity correlated with a significantly greater Day 21 tumor weight to total body weight ratio in the allogeneic group (0.33) compared with the syngeneic (0.25) and saline (0.28) groups P < 0.05). Median host survival was reduced in the allogeneic group (24 days) compared with the syngeneic (30 days) and saline (31 days) groups. There were no significant differences in cellular immunity, tumor growth, or survival between syngeneic and saline control groups. Allogeneic blood transfusion had an adverse affect on NK and T-lymphocyte function which was associated with enhanced tumor growth and reduced survival in tumor-bearing mice.
UR - http://www.scopus.com/inward/record.url?scp=0025354397&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025354397&partnerID=8YFLogxK
U2 - 10.1016/0022-4804(90)90020-3
DO - 10.1016/0022-4804(90)90020-3
M3 - Article
C2 - 2352426
AN - SCOPUS:0025354397
SN - 0022-4804
VL - 48
SP - 498
EP - 503
JO - Journal of Surgical Research
JF - Journal of Surgical Research
IS - 5
ER -