Transient increases in dendritic spine density contribute to dentate gyrus long-term potentiation

Marlena Wosiski-Kuhn, Alexis M. Stranahan

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Dendritic spines are the primary sites for excitatory neurotransmission in the adult brain and exhibit changes in their number and morphology with experience. The relationship between spine formation and synaptic activity has been best characterized along the apical dendrites of pyramidal neurons in the hippocampal CA1 subfield. However, less is known about the structural mechanisms at the spine that mediate plasticity in other hippocampal subfields. The dentate gyrus is the predominant point of entry for synaptic input to the hippocampus, and dentate granule cells differ from CA1 pyramidal neurons in terms of their morphology and biophysical properties. In order to understand the structural mechanisms for plasticity in the dentate gyrus, we measured dendritic spine density in hippocampal slice preparations at different intervals following synaptic stimulation. We observed that transient increases in dendritic spine density are detectable 30 min after induction of long-term potentiation (LTP). By 60 min poststimulation, dendritic spine density has returned to basal levels. Both early LTP and enhancements in dendritic spine density could beblocked by destabilizing actin filaments, but not by inhibitors of transcription or protein synthesis. These results indicate that spine formation is a transient event that is required for dentate gyrus LTP.

Original languageEnglish (US)
Pages (from-to)661-664
Number of pages4
JournalSynapse
Volume66
Issue number7
DOIs
StatePublished - Jul 2012

Keywords

  • Cytoskeleton
  • Dentate gyrus
  • Medial perforant path
  • Synaptic plasticity

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

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