Transplantation of Cardiac Mesenchymal Stem Cell-Derived Exosomes for Angiogenesis

Chengwei Ju, Youngjun Li, Yan Shen, Yutao Liu, Jingwen Cai, Naifeng Liu, Gengshan Ma, Yaoliang Tang

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

We demonstrated the effects of exosomes secreted by cardiac mesenchymal stem cells (C-MSC-Exo) in protecting acute ischemic myocardium from reperfusion injury. To investigate the effect of exosomes from C-MSC on angiogenesis, we injected C-MSC-Exo or PBS intramuscularly into ischemic hind limb. Blood perfusion of limb was evaluated by laser Doppler Imaging. We observed that ischemic limb treated with C-MSC-Exo exhibits improved blood perfusion compared to ischemic limb treated with PBS at 2 weeks and 1 month after induction of limb ischemia. To explore the potential mechanisms underlying C-MSC-Exo’s angiogenetic effect, we performed microRNA array analysis and identify mmu-miR-7116-5p as the most abundant enriched miRNA detected in C-MSC-Exo. Bioinformatics’ analysis shows that miR-7116-5p negatively regulates protein polyubiquitination. In conclusion, our study demonstrated that intramuscular delivery of C-MSC-Exo after limb ischemia improves blood perfusion, and we identified the most abundant miRNAs that are preferentially enriched in C-MSC-Exo.

Original languageEnglish (US)
JournalJournal of Cardiovascular Translational Research
DOIs
StateAccepted/In press - 2018

Keywords

  • Angiogenesis
  • Cardiac mesenchymal stem cells
  • Exosomes
  • Hind limb ischemia
  • miR-7116-5p

ASJC Scopus subject areas

  • Molecular Medicine
  • Genetics
  • Pharmaceutical Science
  • Cardiology and Cardiovascular Medicine
  • Genetics(clinical)

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