Transplantation of Cardiac Mesenchymal Stem Cell-Derived Exosomes for Angiogenesis

Chengwei Ju, Youngjun Li, Yan Shen, Yutao Liu, Jingwen Cai, Naifeng Liu, Gengshan Ma, Yao Liang Tang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We demonstrated the effects of exosomes secreted by cardiac mesenchymal stem cells (C-MSC-Exo) in protecting acute ischemic myocardium from reperfusion injury. To investigate the effect of exosomes from C-MSC on angiogenesis, we injected C-MSC-Exo or PBS intramuscularly into ischemic hind limb. Blood perfusion of limb was evaluated by laser Doppler Imaging. We observed that ischemic limb treated with C-MSC-Exo exhibits improved blood perfusion compared to ischemic limb treated with PBS at 2 weeks and 1 month after induction of limb ischemia. To explore the potential mechanisms underlying C-MSC-Exo’s angiogenetic effect, we performed microRNA array analysis and identify mmu-miR-7116-5p as the most abundant enriched miRNA detected in C-MSC-Exo. Bioinformatics’ analysis shows that miR-7116-5p negatively regulates protein polyubiquitination. In conclusion, our study demonstrated that intramuscular delivery of C-MSC-Exo after limb ischemia improves blood perfusion, and we identified the most abundant miRNAs that are preferentially enriched in C-MSC-Exo.

Original languageEnglish (US)
JournalJournal of Cardiovascular Translational Research
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

Mesenchymal Stem Cell Transplantation
Exosomes
Extremities
MicroRNAs
Perfusion
Ischemia
Computational Biology
Reperfusion Injury
Mesenchymal Stromal Cells
Myocardium
Lasers

Keywords

  • Angiogenesis
  • Cardiac mesenchymal stem cells
  • Exosomes
  • Hind limb ischemia
  • miR-7116-5p

ASJC Scopus subject areas

  • Molecular Medicine
  • Genetics
  • Pharmaceutical Science
  • Cardiology and Cardiovascular Medicine
  • Genetics(clinical)

Cite this

Transplantation of Cardiac Mesenchymal Stem Cell-Derived Exosomes for Angiogenesis. / Ju, Chengwei; Li, Youngjun; Shen, Yan; Liu, Yutao; Cai, Jingwen; Liu, Naifeng; Ma, Gengshan; Tang, Yao Liang.

In: Journal of Cardiovascular Translational Research, 01.01.2018.

Research output: Contribution to journalArticle

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AU - Ma, Gengshan

AU - Tang, Yao Liang

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