Transplantation of cultured choroid plexus epithelial cells via cerebrospinal fluid shows prominent neuroprotective effects against acute ischemic brain injury in the rat

Naoya Matsumoto, Akihiko Taguchi, Hitoshi Kitayama, Yumi Watanabe, Masayoshi Ohta, Tomoyuki Yoshihara, Yutaka Itokazu, Mari Dezawa, Yoshihisa Suzuki, Hisashi Sugimoto, Makoto Noda, Chizuka Ide

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Choroid plexus (CP) epithelial cells (CPECs) produce cerebrospinal fluid (CSF) to provide the CNS with a specialized microenvironment. Our previous study showed that the conditioned medium of cultured CPECs enhanced the survival and neurite extension of hippocampal neurons. The present study examined the ability of cultured CPECs to protect against ischemic brain injury when transplanted into the CSF. Rats were subjected to a transient occlusion of the middle cerebral artery, followed by an injection of cultured CPECs into the fourth ventricle. The injection markedly reduced neurological deficits and infarction volume within 24 h. Other beneficial effects were (1) a reduction in number of apoptotic and inflammatory cells, (2) an up-regulation of the mRNA expression of an anti-apoptotic effecter, cAMP-response element binding protein, and (3) a down-regulation of the production of pro-inflammatory factors such as interleukin-1 beta and inducible nitric oxide synthase. The injected CPECs were located within the ventricles and on the brain's surface, not in the ischemic foci, suggesting that they exert their effects by releasing diffusible neuroprotective factors into the CSF. The transplantation of CPECs via CSF is a potential new strategy for protecting against ischemic brain injury.

Original languageEnglish (US)
Pages (from-to)283-288
Number of pages6
JournalNeuroscience Letters
Volume469
Issue number3
DOIs
StatePublished - Jan 29 2010

Fingerprint

Choroid Plexus
Neuroprotective Agents
Brain Injuries
Cerebrospinal Fluid
Transplantation
Epithelial Cells
Fourth Ventricle
Cyclic AMP Response Element-Binding Protein
Injections
Middle Cerebral Artery Infarction
Nitric Oxide Synthase Type II
Neurites
Conditioned Culture Medium
Interleukin-1beta
Infarction
Cell Survival
Up-Regulation
Down-Regulation
Neurons
Messenger RNA

Keywords

  • Cell transplantation
  • Cerebrospinal fluid
  • Choroid plexus epithelial cell
  • Ischemic brain injury
  • Neuroprotection

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Transplantation of cultured choroid plexus epithelial cells via cerebrospinal fluid shows prominent neuroprotective effects against acute ischemic brain injury in the rat. / Matsumoto, Naoya; Taguchi, Akihiko; Kitayama, Hitoshi; Watanabe, Yumi; Ohta, Masayoshi; Yoshihara, Tomoyuki; Itokazu, Yutaka; Dezawa, Mari; Suzuki, Yoshihisa; Sugimoto, Hisashi; Noda, Makoto; Ide, Chizuka.

In: Neuroscience Letters, Vol. 469, No. 3, 29.01.2010, p. 283-288.

Research output: Contribution to journalArticle

Matsumoto, N, Taguchi, A, Kitayama, H, Watanabe, Y, Ohta, M, Yoshihara, T, Itokazu, Y, Dezawa, M, Suzuki, Y, Sugimoto, H, Noda, M & Ide, C 2010, 'Transplantation of cultured choroid plexus epithelial cells via cerebrospinal fluid shows prominent neuroprotective effects against acute ischemic brain injury in the rat', Neuroscience Letters, vol. 469, no. 3, pp. 283-288. https://doi.org/10.1016/j.neulet.2009.09.060
Matsumoto, Naoya ; Taguchi, Akihiko ; Kitayama, Hitoshi ; Watanabe, Yumi ; Ohta, Masayoshi ; Yoshihara, Tomoyuki ; Itokazu, Yutaka ; Dezawa, Mari ; Suzuki, Yoshihisa ; Sugimoto, Hisashi ; Noda, Makoto ; Ide, Chizuka. / Transplantation of cultured choroid plexus epithelial cells via cerebrospinal fluid shows prominent neuroprotective effects against acute ischemic brain injury in the rat. In: Neuroscience Letters. 2010 ; Vol. 469, No. 3. pp. 283-288.
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