Two cannula method for parenteral infusion and serial blood sampling in the freely moving rat

M. T. Moslen, M. F. Kanz, J. Bhatia, E. M. Catarau

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Materials and techniques are described for positioning and exteriorizing two chronic venous cannulas in the rat. A cannula for fluid infusion is placed in the inferior vena cava via the femoral vein and exteriorized on the tail, whereas a cannula for blood withdrawal is placed in the superior vena cava and exteriorized in the scapular region. Grooming behavior and range of motion are not impeded since the cannula used for chronic infusion is shielded by a light-weight tail cover instead of a torso harness. Application of this method can minimize numbers of rats used in total parenteral nutrition (TPN) studies as demonstrated our feasibility study in which serum parameters of liver function were serially monitored in toxin-treated rats infused with nutrient mixtures.

Original languageEnglish (US)
Pages (from-to)633-638
Number of pages6
JournalJournal of Parenteral and Enteral Nutrition
Volume12
Issue number6
DOIs
StatePublished - 1988

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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