Two distinct arginine methyltransferases are required for biogenesis of Sm-class ribonucleoproteins

Graydon B. Gonsalvez, Liping Tian, Jason K. Ospina, François Michel Boisvert, Angus I. Lamond, A. Gregory Matera

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

Small nuclear ribonucleoproteins (snRNPs) are core components of the spliceosome. The U1, U2, U4, and U5 snRNPs each contain a common set of seven Sm proteins. Three of these Sm proteins are posttranslationally modified to contain symmetric dimethylarginine (sDMA) residues within their C-terminal tails. However, the precise function of this modification in the snRNP biogenesis pathway is unclear. Several lines of evidence suggest that the methyltransferase protein arginine methyltransferase 5 (PRMT5) is responsible for sDMA modification of Sm proteins. We found that in human cells, PRMT5 and a newly discovered type II methyltransferase, PRMT7, are each required for Sm protein sDMA modification. Furthermore, we show that the two enzymes function nonredundantly in Sm protein methylation. Lastly, we provide in vivo evidence demonstrating that Sm protein sDMA modification is required for snRNP biogenesis in human cells.

Original languageEnglish (US)
Pages (from-to)733-740
Number of pages8
JournalJournal of Cell Biology
Volume178
Issue number5
DOIs
StatePublished - Aug 27 2007

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Ribonucleoproteins
Small Nuclear Ribonucleoproteins
Proteins
U4-U6 Small Nuclear Ribonucleoproteins
Phosphatidyl-N-Methylethanolamine N-Methyltransferase
U5 Small Nuclear Ribonucleoproteins
U2 Small Nuclear Ribonucleoproteins
Protein-Arginine N-Methyltransferases
U1 Small Nuclear Ribonucleoproteins
Spliceosomes
Methyltransferases
Methylation
human PRMT2 protein
Tail
N,N'-dimethylarginine
Enzymes

ASJC Scopus subject areas

  • Cell Biology

Cite this

Two distinct arginine methyltransferases are required for biogenesis of Sm-class ribonucleoproteins. / Gonsalvez, Graydon B.; Tian, Liping; Ospina, Jason K.; Boisvert, François Michel; Lamond, Angus I.; Matera, A. Gregory.

In: Journal of Cell Biology, Vol. 178, No. 5, 27.08.2007, p. 733-740.

Research output: Contribution to journalArticle

Gonsalvez, Graydon B. ; Tian, Liping ; Ospina, Jason K. ; Boisvert, François Michel ; Lamond, Angus I. ; Matera, A. Gregory. / Two distinct arginine methyltransferases are required for biogenesis of Sm-class ribonucleoproteins. In: Journal of Cell Biology. 2007 ; Vol. 178, No. 5. pp. 733-740.
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