Two functionally distinct sources of actin monomers supply the leading edge of lamellipodia

Eric A. Vitriol, Laura M. McMillen, Maryna Kapustina, Shawn M. Gomez, Dimitrios Vavylonis, James Q. Zheng

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

Lamellipodia, the sheet-like protrusions of motile cells, consist of networks of actin filaments (F-actin) regulated by the ordered assembly from and disassembly into actin monomers (G-actin). Traditionally, G-actin is thought to exist as a homogeneous pool. Here, we show that there are two functionally and molecularly distinct sources of G-actin that supply lamellipodial actin networks. G-actin originating from the cytosolic pool requires the monomer-binding protein thymosin β4 (Tβ4) for optimal leading-edge localization, is targeted to formins, and is responsible for creating an elevated G/F-actin ratio that promotes membrane protrusion. The second source of G-actin comes from recycled lamellipodia F-actin. Recycling occurs independently of Tβ4 and appears to regulate lamellipodia homeostasis. Tβ4-bound G-actin specifically localizes to the leading edge because it does not interact with Arp2/3-mediated polymerization sites found throughout the lamellipodia. These findings demonstrate that actin networks can be constructed from multiple sources of monomers with discrete spatiotemporal functions.

Original languageEnglish (US)
Pages (from-to)433-445
Number of pages13
JournalCell Reports
Volume11
Issue number3
DOIs
StatePublished - Apr 21 2015
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint

Dive into the research topics of 'Two functionally distinct sources of actin monomers supply the leading edge of lamellipodia'. Together they form a unique fingerprint.

Cite this