TY - JOUR
T1 - Two-hybrid analysis of human salivary mucin MUC7 interactions
AU - Bruno, Lucila S.
AU - Li, Xiaojing
AU - Wang, Li
AU - Soares, Rodrigo V.
AU - Siqueira Nogueira, Camille Colares
AU - Oppenheim, Frank G.
AU - Troxler, Robert F.
AU - Offner, Gwynneth D.
PY - 2005/10/30
Y1 - 2005/10/30
N2 - MUC7 is a low molecular weight monomeric mucin secreted by submandibular, sublingual and minor salivary glands. This mucin has been implicated in the non-immune host defense system in the oral cavity since it binds and agglutinates a variety of oral microbes. To investigate interactions between this mucin and other secretory salivary proteins, a submandibular gland prey library was screened with baits encoding the N- and C-terminal regions of MUC7 in the yeast two-hybrid system. The N-terminal region interacted with several secretory salivary proteins, whereas the C-terminal region did not. Interacting proteins included amylase, acidic proline-rich protein 2, basic proline-rich protein 3, lacrimal proline-rich protein 4, statherin and histatin 1. Formation of complexes between these proteins and the N-terminal region of MUC7 was confirmed in Far Western blotting experiments. Interactions between mucin and non-mucin proteins in saliva could protect complex partners from proteolysis, modulate the biological activity of complexed proteins or serve as a delivery system for distribution of secretory salivary proteins throughout the oral cavity.
AB - MUC7 is a low molecular weight monomeric mucin secreted by submandibular, sublingual and minor salivary glands. This mucin has been implicated in the non-immune host defense system in the oral cavity since it binds and agglutinates a variety of oral microbes. To investigate interactions between this mucin and other secretory salivary proteins, a submandibular gland prey library was screened with baits encoding the N- and C-terminal regions of MUC7 in the yeast two-hybrid system. The N-terminal region interacted with several secretory salivary proteins, whereas the C-terminal region did not. Interacting proteins included amylase, acidic proline-rich protein 2, basic proline-rich protein 3, lacrimal proline-rich protein 4, statherin and histatin 1. Formation of complexes between these proteins and the N-terminal region of MUC7 was confirmed in Far Western blotting experiments. Interactions between mucin and non-mucin proteins in saliva could protect complex partners from proteolysis, modulate the biological activity of complexed proteins or serve as a delivery system for distribution of secretory salivary proteins throughout the oral cavity.
KW - MG2
KW - Mucin
KW - Protein-protein interaction
KW - Salivary protein
UR - http://www.scopus.com/inward/record.url?scp=26944477384&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=26944477384&partnerID=8YFLogxK
U2 - 10.1016/j.bbamcr.2005.08.007
DO - 10.1016/j.bbamcr.2005.08.007
M3 - Article
C2 - 16203048
AN - SCOPUS:26944477384
VL - 1746
SP - 65
EP - 72
JO - Biochimica et Biophysica Acta - Molecular Cell Research
JF - Biochimica et Biophysica Acta - Molecular Cell Research
SN - 0167-4889
IS - 1
ER -