Two-hybrid analysis of human salivary mucin MUC7 interactions

Lucila S. Bruno, Xiaojing Li, Li Wang, Rodrigo V. Soares, Camille Colares Siqueira Nogueira, Frank G. Oppenheim, Robert F. Troxler, Gwynneth D. Offner

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

MUC7 is a low molecular weight monomeric mucin secreted by submandibular, sublingual and minor salivary glands. This mucin has been implicated in the non-immune host defense system in the oral cavity since it binds and agglutinates a variety of oral microbes. To investigate interactions between this mucin and other secretory salivary proteins, a submandibular gland prey library was screened with baits encoding the N- and C-terminal regions of MUC7 in the yeast two-hybrid system. The N-terminal region interacted with several secretory salivary proteins, whereas the C-terminal region did not. Interacting proteins included amylase, acidic proline-rich protein 2, basic proline-rich protein 3, lacrimal proline-rich protein 4, statherin and histatin 1. Formation of complexes between these proteins and the N-terminal region of MUC7 was confirmed in Far Western blotting experiments. Interactions between mucin and non-mucin proteins in saliva could protect complex partners from proteolysis, modulate the biological activity of complexed proteins or serve as a delivery system for distribution of secretory salivary proteins throughout the oral cavity.

Original languageEnglish (US)
Pages (from-to)65-72
Number of pages8
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1746
Issue number1
DOIs
StatePublished - Oct 30 2005
Externally publishedYes

Keywords

  • MG2
  • Mucin
  • Protein-protein interaction
  • Salivary protein

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Two-hybrid analysis of human salivary mucin MUC7 interactions'. Together they form a unique fingerprint.

  • Cite this

    Bruno, L. S., Li, X., Wang, L., Soares, R. V., Siqueira Nogueira, C. C., Oppenheim, F. G., Troxler, R. F., & Offner, G. D. (2005). Two-hybrid analysis of human salivary mucin MUC7 interactions. Biochimica et Biophysica Acta - Molecular Cell Research, 1746(1), 65-72. https://doi.org/10.1016/j.bbamcr.2005.08.007