Two-photon laser confocal microscopy of micropermeability of resin-dentin bonds made with water or ethanol wet bonding

Salvatore Sauro, Timothy F. Watson, Francesco Mannocci, Katsuya Miyake, Bradford P. Huffman, Franklin Chi Meng Tay, David Henry Pashley

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

This study evaluated the micropermeability of six etch-and-rinse adhesives bonded to dentin. There were two principal groups: wet bonding with water or wet bonding with absolute ethyl alcohol. After bonding and the creation of composite build-ups, the pulp chambers were filled with 0.1% lucifer yellow. The contents of the pulp chamber were kept under 20 cm H2O pressure to simulate pulpal pressure for 3 h. The specimens were vertically sectioned into multiple 0.5-mm thick slabs that were polished and then examined using a twophoton confocal laser scanning microscope (TPCLSM). The results showed that specimens bonded with adhesives using the water wet-bonding condition all showed tracer taken up uniformly by the hybrid layer. This uptake of fluorescent tracer into the hybrid layer was quantified by computer software. The most hydrophobic experimental resins showed the highest fluorescent tracer uptake (ca. 1800 ± 160 arbitrary fluorescent units/std. surface area). The most hydrophilic experimental resins showed the lowest tracer uptake into watersaturated hybrid layers. When ethanol wet-bonding was used, significantly less fluorescent tracer was seen in hybrid layers. The most hydrophilic experimental resins and Single Bond Plus showed little micropermeability. Clearly, ethanol wet-bonding seals dentin significantly better than water-wet dentin regardless of the adhesive in etch-and-rinse systems.

Original languageEnglish (US)
Pages (from-to)327-337
Number of pages11
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Volume90 B
Issue number1
DOIs
StatePublished - Jan 1 2009

Fingerprint

Confocal microscopy
Dentin
Photons
Confocal Microscopy
Ethanol
Resins
Adhesives
Water
Lasers
Dental Pulp Cavity
Pressure
Pulp
Software
Seals
Microscopes
Scanning
Composite materials

Keywords

  • Dentin sealing
  • Ethanol-saturated dentin
  • Hydrophobic hybrid layer
  • Micropermeability
  • Two-photon confocal microscopy

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

Cite this

Two-photon laser confocal microscopy of micropermeability of resin-dentin bonds made with water or ethanol wet bonding. / Sauro, Salvatore; Watson, Timothy F.; Mannocci, Francesco; Miyake, Katsuya; Huffman, Bradford P.; Tay, Franklin Chi Meng; Pashley, David Henry.

In: Journal of Biomedical Materials Research - Part B Applied Biomaterials, Vol. 90 B, No. 1, 01.01.2009, p. 327-337.

Research output: Contribution to journalArticle

Sauro, Salvatore ; Watson, Timothy F. ; Mannocci, Francesco ; Miyake, Katsuya ; Huffman, Bradford P. ; Tay, Franklin Chi Meng ; Pashley, David Henry. / Two-photon laser confocal microscopy of micropermeability of resin-dentin bonds made with water or ethanol wet bonding. In: Journal of Biomedical Materials Research - Part B Applied Biomaterials. 2009 ; Vol. 90 B, No. 1. pp. 327-337.
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