Two-step protocol for isolation and culture of cardiospheres

Lijuan Chen, Yaohua Pan, Lan Zhang, Yingjie Wang, Neal Weintraub, Yaoliang Tang

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Scopus citations

Abstract

Cardiac progenitor cells (CPC) are a unique pool of progenitor cells residing in the heart that play an important role in cardiac homeostasis and physiological cardiovascular cell turnover during acute myocardial infarction (MI). Transplanting CPC into the heart has shown promise in two recent clinical trials of cardiac repair (SCIPIO & CADUCEUS). CSCs were originally isolated directly from enzymatically digested hearts followed by cell sorting using stem cell markers. However, long exposure to enzymatic digestion can affect the integrity of stem cell markers on the cell surface and also compromise stem cell function. Here, we describe a two-step procedure in which a large number of intact cardiac progenitor cells can be purified from small amount of heart tissue.

Original languageEnglish (US)
Title of host publicationCellular Cardiomyoplasty
Subtitle of host publicationMethods and Protocols
Pages75-80
Number of pages6
DOIs
StatePublished - Jul 18 2013
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1036
ISSN (Print)1064-3745

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Keywords

  • Cardiac progenitor cells
  • Cardiosphere
  • Magnetic-activated cell sorting (MACS)

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Chen, L., Pan, Y., Zhang, L., Wang, Y., Weintraub, N., & Tang, Y. (2013). Two-step protocol for isolation and culture of cardiospheres. In Cellular Cardiomyoplasty: Methods and Protocols (pp. 75-80). (Methods in Molecular Biology; Vol. 1036). https://doi.org/10.1007/978-1-62703-511-8_6