Type 2 diabetes severely impairs structural and functional adaptation of rat resistance arteries to chronic changes in blood flow

Eric Jacques Belin de Chantemele, Emilie Vessières, Anne Laure Guihot, Bertrand Toutain, Maud Maquignau, Laurent Loufrani, Daniel Henrion

Research output: Contribution to journalArticle

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Abstract

Aims: Endothelial dysfunction in resistance arteries (RAs) leads to end-organ damage in type 2 diabetes. Remodelling of RAs in response to chronic increases in blood flow depends on the integrity of the endothelium. Since type 2 diabetes impairs endothelial sensitivity to flow and increases oxidative stress, we hypothesized that flow-induced remodelling in RAs would be impaired in diabetes. Thus, we studied the structural and functional adaptation of RAs from Zucker diabetic fatty (ZDF) and lean Zucker (LZ) rats to chronic changes in flow. Methods and results: Mesenteric RAs were alternatively ligated so that one artery was submitted to high flow (HF) and compared with normal-flow (NF) arteries located at distance. After 3 weeks, arteries were studied in vitro (n = 10 rats per group). Arterial diameter (468 vs. 394 ± 8 μm) and endothelial (acetylcholine)-dependent dilation (91 ± 8 vs. 75 ± 6% dilation) were higher in HF than in NF arteries in LZ rats. In ZDF rats, diameter (396 ± 9 vs. 440 ± 17 μm) and acetylcholine-mediated dilation (42 ± 8 vs. 75 ± 7%) were lower in HF than in NF arteries. Nevertheless, endothelial NO synthase and NADP(H) oxidase subunits (gp91, p67) expression level and superoxide production (dihydroethidium staining) were higher in HF than in NF arteries in both strains, suggesting an efficient flow-sensing process in ZDF rats. In ZDF rats, basal oxidative stress was higher compared with LZ rats: dihydroethidium staining was higher in NF and HF arteries from ZDF rats, and acetylcholine-mediated dilation was improved by an acute antioxidant (tempol) in NF and HF arteries from ZDF rats. Thus, superoxide overproduction in ZDF rats impaired NO-dependent dilation and HF remodelling. Indeed, a chronic treatment with tempol increased HF artery diameter and endothelium-dependent dilation in ZDF rats. Conclusion: In type 2 diabetic rats, a chronic increase in blood flow failed to induce outward remodelling and to improve endothelium-dependent dilation, mainly because of superoxide overproduction.

Original languageEnglish (US)
Pages (from-to)788-796
Number of pages9
JournalCardiovascular Research
Volume81
Issue number4
DOIs
StatePublished - Mar 1 2009

Fingerprint

Type 2 Diabetes Mellitus
Arteries
Dilatation
Zucker Rats
Superoxides
Acetylcholine
Endothelium
Oxidative Stress
Staining and Labeling
Mesenteric Arteries
NADP
Nitric Oxide Synthase
Oxidoreductases
Antioxidants

Keywords

  • Blood flow
  • Endothelium
  • Nitric oxide
  • Reactive oxygen species
  • Resistance arteries
  • Shear stress
  • Type 2 diabetes
  • Zucker diabetic fatty rats

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Type 2 diabetes severely impairs structural and functional adaptation of rat resistance arteries to chronic changes in blood flow. / Belin de Chantemele, Eric Jacques; Vessières, Emilie; Guihot, Anne Laure; Toutain, Bertrand; Maquignau, Maud; Loufrani, Laurent; Henrion, Daniel.

In: Cardiovascular Research, Vol. 81, No. 4, 01.03.2009, p. 788-796.

Research output: Contribution to journalArticle

Belin de Chantemele, Eric Jacques ; Vessières, Emilie ; Guihot, Anne Laure ; Toutain, Bertrand ; Maquignau, Maud ; Loufrani, Laurent ; Henrion, Daniel. / Type 2 diabetes severely impairs structural and functional adaptation of rat resistance arteries to chronic changes in blood flow. In: Cardiovascular Research. 2009 ; Vol. 81, No. 4. pp. 788-796.
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AU - Vessières, Emilie

AU - Guihot, Anne Laure

AU - Toutain, Bertrand

AU - Maquignau, Maud

AU - Loufrani, Laurent

AU - Henrion, Daniel

PY - 2009/3/1

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N2 - Aims: Endothelial dysfunction in resistance arteries (RAs) leads to end-organ damage in type 2 diabetes. Remodelling of RAs in response to chronic increases in blood flow depends on the integrity of the endothelium. Since type 2 diabetes impairs endothelial sensitivity to flow and increases oxidative stress, we hypothesized that flow-induced remodelling in RAs would be impaired in diabetes. Thus, we studied the structural and functional adaptation of RAs from Zucker diabetic fatty (ZDF) and lean Zucker (LZ) rats to chronic changes in flow. Methods and results: Mesenteric RAs were alternatively ligated so that one artery was submitted to high flow (HF) and compared with normal-flow (NF) arteries located at distance. After 3 weeks, arteries were studied in vitro (n = 10 rats per group). Arterial diameter (468 vs. 394 ± 8 μm) and endothelial (acetylcholine)-dependent dilation (91 ± 8 vs. 75 ± 6% dilation) were higher in HF than in NF arteries in LZ rats. In ZDF rats, diameter (396 ± 9 vs. 440 ± 17 μm) and acetylcholine-mediated dilation (42 ± 8 vs. 75 ± 7%) were lower in HF than in NF arteries. Nevertheless, endothelial NO synthase and NADP(H) oxidase subunits (gp91, p67) expression level and superoxide production (dihydroethidium staining) were higher in HF than in NF arteries in both strains, suggesting an efficient flow-sensing process in ZDF rats. In ZDF rats, basal oxidative stress was higher compared with LZ rats: dihydroethidium staining was higher in NF and HF arteries from ZDF rats, and acetylcholine-mediated dilation was improved by an acute antioxidant (tempol) in NF and HF arteries from ZDF rats. Thus, superoxide overproduction in ZDF rats impaired NO-dependent dilation and HF remodelling. Indeed, a chronic treatment with tempol increased HF artery diameter and endothelium-dependent dilation in ZDF rats. Conclusion: In type 2 diabetic rats, a chronic increase in blood flow failed to induce outward remodelling and to improve endothelium-dependent dilation, mainly because of superoxide overproduction.

AB - Aims: Endothelial dysfunction in resistance arteries (RAs) leads to end-organ damage in type 2 diabetes. Remodelling of RAs in response to chronic increases in blood flow depends on the integrity of the endothelium. Since type 2 diabetes impairs endothelial sensitivity to flow and increases oxidative stress, we hypothesized that flow-induced remodelling in RAs would be impaired in diabetes. Thus, we studied the structural and functional adaptation of RAs from Zucker diabetic fatty (ZDF) and lean Zucker (LZ) rats to chronic changes in flow. Methods and results: Mesenteric RAs were alternatively ligated so that one artery was submitted to high flow (HF) and compared with normal-flow (NF) arteries located at distance. After 3 weeks, arteries were studied in vitro (n = 10 rats per group). Arterial diameter (468 vs. 394 ± 8 μm) and endothelial (acetylcholine)-dependent dilation (91 ± 8 vs. 75 ± 6% dilation) were higher in HF than in NF arteries in LZ rats. In ZDF rats, diameter (396 ± 9 vs. 440 ± 17 μm) and acetylcholine-mediated dilation (42 ± 8 vs. 75 ± 7%) were lower in HF than in NF arteries. Nevertheless, endothelial NO synthase and NADP(H) oxidase subunits (gp91, p67) expression level and superoxide production (dihydroethidium staining) were higher in HF than in NF arteries in both strains, suggesting an efficient flow-sensing process in ZDF rats. In ZDF rats, basal oxidative stress was higher compared with LZ rats: dihydroethidium staining was higher in NF and HF arteries from ZDF rats, and acetylcholine-mediated dilation was improved by an acute antioxidant (tempol) in NF and HF arteries from ZDF rats. Thus, superoxide overproduction in ZDF rats impaired NO-dependent dilation and HF remodelling. Indeed, a chronic treatment with tempol increased HF artery diameter and endothelium-dependent dilation in ZDF rats. Conclusion: In type 2 diabetic rats, a chronic increase in blood flow failed to induce outward remodelling and to improve endothelium-dependent dilation, mainly because of superoxide overproduction.

KW - Blood flow

KW - Endothelium

KW - Nitric oxide

KW - Reactive oxygen species

KW - Resistance arteries

KW - Shear stress

KW - Type 2 diabetes

KW - Zucker diabetic fatty rats

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