Tyrosine kinase activity may be necessary but is not sufficient for c- erbB1-mediated tissue-specific tumorigenicity

D. C. Connolly, S. L. Toutenhoofd, N. J. Maihle

Research output: Contribution to journalComment/debate

13 Scopus citations

Abstract

Expression of mutant avian c-erbB1 genes results in tissue-specific transformation in chickens. Site-directed mutagenesis was used to generate kinase-defective mutants of several tissue-specific v-erbB transforming mutants by replacement of the ATP-binding lysine residue in the kinase domain with an arginine residue. These kinase-defective v-erbB mutants were analyzed for their in vitro and in vivo transforming potentials. Specifically, kinase- defective mutants of erythroleukemogenic, hemangioma-inducing, and sarcomagenic v-erbB genes were assessed for their oncogenic potential. In vitro transformation potential was assessed by soft-agar colony formation in primary cultures of chick embryo fibroblasts (CEF). In vivo transformation potential was determined by infection of 1-day-old line 0 chicks with concentrated recombinant retrovirus and then monitoring of birds for tumor formation. These transformation assays demonstrate that kinase activity is absolutely essential for transformation by tissue-specific transforming mutants of the avian c-erbB1 gene. Since all of the tissue-specific v-erbB mutants characterized to date exhibit tyrosine kinase activity in vitro but do not transform all tissues in which they are expressed, we conclude that v- erbB-associated tyrosine kinase activity may be necessary but is not sufficient to induce tumor formation.

Original languageEnglish (US)
Pages (from-to)6804-6810
Number of pages7
JournalJournal of Virology
Volume68
Issue number10
DOIs
StatePublished - Jan 1 1994

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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