UFMylation of RPL26 links translocation-associated quality control to endoplasmic reticulum protein homeostasis

Lihui Wang, Yue Xu, Heather Rogers, Layla Saidi, Constance Tom Noguchi, Honglin Li, Jonathan Wilson Yewdell, Nicholas Raymond Guydosh, Yihong Ye

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Protein biogenesis at the endoplasmic reticulum (ER) in eukaryotic cells is monitored by a protein quality control system named ER-associated protein degradation (ERAD). While there has been substantial progress in understanding how ERAD eliminates defective polypeptides generated from erroneous folding, how cells remove nascent chains stalled in the translocon during co-translational protein insertion into the ER is unclear. Here we show that ribosome stalling during protein translocation induces the attachment of UFM1, a ubiquitin-like modifier, to two conserved lysine residues near the COOH-terminus of the 60S ribosomal subunit RPL26 (uL24) at the ER. Strikingly, RPL26 UFMylation enables the degradation of stalled nascent chains, but unlike ERAD or previously established cytosolic ribosome-associated quality control (RQC), which uses proteasome to degrade their client proteins, ribosome UFMylation promotes the targeting of a translocation-arrested ER protein to lysosomes for degradation. RPL26 UFMylation is upregulated during erythroid differentiation to cope with increased secretory flow, and compromising UFMylation impairs protein secretion, and ultimately hemoglobin production. We propose that in metazoan, co-translational protein translocation into the ER is safeguarded by a UFMylation-dependent protein quality control mechanism, which when impaired causes anemia in mice and abnormal neuronal development in humans.

Original languageEnglish (US)
Pages (from-to)5-20
Number of pages16
JournalCell Research
Volume30
Issue number1
DOIs
StatePublished - Jan 1 2020

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'UFMylation of RPL26 links translocation-associated quality control to endoplasmic reticulum protein homeostasis'. Together they form a unique fingerprint.

  • Cite this

    Wang, L., Xu, Y., Rogers, H., Saidi, L., Noguchi, C. T., Li, H., Yewdell, J. W., Guydosh, N. R., & Ye, Y. (2020). UFMylation of RPL26 links translocation-associated quality control to endoplasmic reticulum protein homeostasis. Cell Research, 30(1), 5-20. https://doi.org/10.1038/s41422-019-0236-6