Ultrastructural alterations of the mitochondrial ATPase in the calcium paradox as revealed by negative staining

P. M. Rahamathulla, M. Ashraf, S. Sato, John Benedict

Research output: Contribution to journalArticle

5 Scopus citations


We have used a simple negative staining technique to study the structural alterations of mitochondria from biopsies of hearts subjected to the calcium paradox and treatment with diltiazem, a calcium channel blocker. A significant (P<0.05) decrease in the number of spheres on the mitochondrial membranes occurs during the calcium paradox (58.0 ±4. I/μm vs. control 80.5±6.5). Treatment with diltiazem prevented the loss of spheres from mitochondrial membranes during the calcium paradox (75.5 ±5.0 gmm). We found that this negative staining technique can be used for quick assessment of the condition of mitochondria in biopsies from normal and pathological organs.

Original languageEnglish (US)
Pages (from-to)267-272
Number of pages6
JournalVirchows Archiv B Cell Pathology Including Molecular Pathology
Issue number1
Publication statusPublished - Dec 1 1982
Externally publishedYes



  • ATPase
  • Calcium channel blocker
  • Calcium paradox
  • Diltiazem
  • Electron microscopy
  • Mitochondria
  • Negative staining

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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