Ultraviolet B-sensitive rice cultivar deficient in cyclobutyl pyrimidine dimer repair

Jun Hidema, Tadashi Kumagai, John C. Sutherland, Betsy M. Sutherland

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Repair of cyclobutyl pyrimidine dimers (CPDs) in DNA is essential in most organisms to prevent biological damage by ultraviolet (UV) light. In higher plants tested thus far, UV-sensitive strains had higher initial damage levels or deficient repair of nondimer DNA lesions but normal CPD repair. This suggested that CPDs might not be important for biological lesions. The photosynthetic apparatus has also been proposed as a critical target. We have analyzed CPD induction and repair in the UV-sensitive rice (Oryza sativa L.) cultivar Norin 1 and its close relative UV-resistant Sasanishiki using alkaline agarose gel electrophoresis. Norin 1 is deficient in cyclobutyl pyrimidi.ne dimer photoreactivation and excision; thus, UV sensitivity correlates with deficient dimer repair.

Original languageEnglish (US)
Pages (from-to)39-44
Number of pages6
JournalPlant Physiology
Volume113
Issue number1
DOIs
StatePublished - Jan 1 1997
Externally publishedYes

Fingerprint

Pyrimidine Dimers
pyrimidines
ultraviolet radiation
rice
lesions (plant)
cultivars
DNA Repair-Deficiency Disorders
Agar Gel Electrophoresis
Ultraviolet Rays
DNA repair
agarose
gel electrophoresis
Oryza sativa
Oryza
DNA
organisms

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

Ultraviolet B-sensitive rice cultivar deficient in cyclobutyl pyrimidine dimer repair. / Hidema, Jun; Kumagai, Tadashi; Sutherland, John C.; Sutherland, Betsy M.

In: Plant Physiology, Vol. 113, No. 1, 01.01.1997, p. 39-44.

Research output: Contribution to journalArticle

Hidema, Jun ; Kumagai, Tadashi ; Sutherland, John C. ; Sutherland, Betsy M. / Ultraviolet B-sensitive rice cultivar deficient in cyclobutyl pyrimidine dimer repair. In: Plant Physiology. 1997 ; Vol. 113, No. 1. pp. 39-44.
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