Unexpected reactivities of T cells selected by a single MHC-peptide ligand

Nagendra Singh, Luc Van Kaer

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

In H2-DM mutant mice, most MHC class II molecules are bound by a single peptide, CLIP, derived from the class II-associated invariant chain. Previous studies showed that H2-DM- cells are defective in presenting synthetic peptides to class II-restricted T cells. In sharp contrast, however, the same peptides elicited strong CD4+ T cell responses in H2-DM- animals. We now provide an explanation for this apparent discrepancy. Peptide-specific CD4+ T cells from wild-type mice were efficiently stimulated by H2-DM+, but not by H2-DM- cells pulsed with the cognate peptide. In sharp contrast, CD4+ T cells from mutant animals specific for the same MHC-peptide combination recognized peptide-pulsed H2-DM+ and H2-DM- cells equally well. In addition, unlike Ag-specific T cells from wild-type animals, the reactivities of peptide-specific T cells from mutant animals could not be efficiently blocked by Abs specific for the cognate MHC class H-peptide combination. We further demonstrated that the distinct reactivities of CD4+ T cells from H2- DM+ and H2-DM- mice are due to differences in thymic selection. Collectively, these findings indicate that the CD4+ T cell repertoires of H2-DM+ and H2-DM- mice are remarkably different.

Original languageEnglish (US)
Pages (from-to)3583-3591
Number of pages9
JournalJournal of Immunology
Volume163
Issue number7
StatePublished - Oct 1 1999
Externally publishedYes

Fingerprint

Ligands
T-Lymphocytes
Peptides
Peptide T
Wild Animals
invariant chain

ASJC Scopus subject areas

  • Immunology

Cite this

Unexpected reactivities of T cells selected by a single MHC-peptide ligand. / Singh, Nagendra; Van Kaer, Luc.

In: Journal of Immunology, Vol. 163, No. 7, 01.10.1999, p. 3583-3591.

Research output: Contribution to journalArticle

@article{d175a4e49a6e42c6b2184a294b666964,
title = "Unexpected reactivities of T cells selected by a single MHC-peptide ligand",
abstract = "In H2-DM mutant mice, most MHC class II molecules are bound by a single peptide, CLIP, derived from the class II-associated invariant chain. Previous studies showed that H2-DM- cells are defective in presenting synthetic peptides to class II-restricted T cells. In sharp contrast, however, the same peptides elicited strong CD4+ T cell responses in H2-DM- animals. We now provide an explanation for this apparent discrepancy. Peptide-specific CD4+ T cells from wild-type mice were efficiently stimulated by H2-DM+, but not by H2-DM- cells pulsed with the cognate peptide. In sharp contrast, CD4+ T cells from mutant animals specific for the same MHC-peptide combination recognized peptide-pulsed H2-DM+ and H2-DM- cells equally well. In addition, unlike Ag-specific T cells from wild-type animals, the reactivities of peptide-specific T cells from mutant animals could not be efficiently blocked by Abs specific for the cognate MHC class H-peptide combination. We further demonstrated that the distinct reactivities of CD4+ T cells from H2- DM+ and H2-DM- mice are due to differences in thymic selection. Collectively, these findings indicate that the CD4+ T cell repertoires of H2-DM+ and H2-DM- mice are remarkably different.",
author = "Nagendra Singh and {Van Kaer}, Luc",
year = "1999",
month = "10",
day = "1",
language = "English (US)",
volume = "163",
pages = "3583--3591",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "7",

}

TY - JOUR

T1 - Unexpected reactivities of T cells selected by a single MHC-peptide ligand

AU - Singh, Nagendra

AU - Van Kaer, Luc

PY - 1999/10/1

Y1 - 1999/10/1

N2 - In H2-DM mutant mice, most MHC class II molecules are bound by a single peptide, CLIP, derived from the class II-associated invariant chain. Previous studies showed that H2-DM- cells are defective in presenting synthetic peptides to class II-restricted T cells. In sharp contrast, however, the same peptides elicited strong CD4+ T cell responses in H2-DM- animals. We now provide an explanation for this apparent discrepancy. Peptide-specific CD4+ T cells from wild-type mice were efficiently stimulated by H2-DM+, but not by H2-DM- cells pulsed with the cognate peptide. In sharp contrast, CD4+ T cells from mutant animals specific for the same MHC-peptide combination recognized peptide-pulsed H2-DM+ and H2-DM- cells equally well. In addition, unlike Ag-specific T cells from wild-type animals, the reactivities of peptide-specific T cells from mutant animals could not be efficiently blocked by Abs specific for the cognate MHC class H-peptide combination. We further demonstrated that the distinct reactivities of CD4+ T cells from H2- DM+ and H2-DM- mice are due to differences in thymic selection. Collectively, these findings indicate that the CD4+ T cell repertoires of H2-DM+ and H2-DM- mice are remarkably different.

AB - In H2-DM mutant mice, most MHC class II molecules are bound by a single peptide, CLIP, derived from the class II-associated invariant chain. Previous studies showed that H2-DM- cells are defective in presenting synthetic peptides to class II-restricted T cells. In sharp contrast, however, the same peptides elicited strong CD4+ T cell responses in H2-DM- animals. We now provide an explanation for this apparent discrepancy. Peptide-specific CD4+ T cells from wild-type mice were efficiently stimulated by H2-DM+, but not by H2-DM- cells pulsed with the cognate peptide. In sharp contrast, CD4+ T cells from mutant animals specific for the same MHC-peptide combination recognized peptide-pulsed H2-DM+ and H2-DM- cells equally well. In addition, unlike Ag-specific T cells from wild-type animals, the reactivities of peptide-specific T cells from mutant animals could not be efficiently blocked by Abs specific for the cognate MHC class H-peptide combination. We further demonstrated that the distinct reactivities of CD4+ T cells from H2- DM+ and H2-DM- mice are due to differences in thymic selection. Collectively, these findings indicate that the CD4+ T cell repertoires of H2-DM+ and H2-DM- mice are remarkably different.

UR - http://www.scopus.com/inward/record.url?scp=0033213947&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033213947&partnerID=8YFLogxK

M3 - Article

C2 - 10490950

AN - SCOPUS:0033213947

VL - 163

SP - 3583

EP - 3591

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 7

ER -