Upregulation of cannabinoid receptor-1 and fibrotic activation of mouse hepatic stellate cells during Schistosoma J. infection: Role of NADPH oxidase

Mi Wang, Justine M. Abais, Nan Meng, Yang Zhang, Joseph K. Ritter, Pin Lan Li, Wang Xian Tang

Research output: Contribution to journalArticle

Abstract

The endocannabinoid system (CS) has been implicated in the development of hepatic fibrosis such as schistosomiasis-associated liver fibrosis (SSLF). However, the mechanisms mediating the action of the CS in hepatic fibrosis are unclear. The present study hypothesized that Schistosoma J. infection upregulates cannabinoid receptor 1 (CB1) due to activation of NADPH oxidase leading to a fibrotic phenotype in hepatic stellate cells (HSCs). The SSLF model was developed by infecting mice with Schistosoma J. cercariae in the skin, and HSCs from control and infected mice were then isolated, cultured, and confirmed by analysis of HSC markers α-SMA and desmin. CB1 significantly increased in HSCs isolated from mice with SSLF, which was accompanied by a greater expression of fibrotic markers α-SMA, collagen I, and TIMP-1. CB1 upregulation and enhanced fibrotic changes were also observed in normal HSCs treated with soluble egg antigen (SEA) from Schistosoma J. Electron spin resonance (ESR) analysis further demonstrated that superoxide (O 2•-) production was increased in infected HSCs or normal HSCs stimulated with SEA. Both Nox4 and Nox1 siRNA prevented SEA-induced upregulation of CB1, α-SMA, collagen I, and TIMP-1 by inhibition of O 2•- production, while CB1 siRNA blocked SEA-induced fibrotic changes without effect on O2•- production in these HSCs. Taken together, these data suggest that the fibrotic activation of HSCs on Schistosoma J. infection or SEA stimulation is associated with NADPH oxidase-mediated redox regulation of CB1 expression, which may be a triggering mechanism for SSLF.

Original languageEnglish (US)
Pages (from-to)109-120
Number of pages12
JournalFree Radical Biology and Medicine
Volume71
DOIs
StatePublished - Jun 2014
Externally publishedYes

Fingerprint

Schistosoma
Cannabinoid Receptors
Hepatic Stellate Cells
NADPH Oxidase
Up-Regulation
Chemical activation
Infection
Schistosomiasis
Ovum
Liver Cirrhosis
Liver
Antigens
Tissue Inhibitor of Metalloproteinase-1
Small Interfering RNA
Fibrosis
Collagen
Cercaria
Endocannabinoids
Desmin
Electron Spin Resonance Spectroscopy

Keywords

  • Cannabinoid receptor-1
  • Hepatic stellate cell
  • NADPH oxidase
  • Schistosomiasis-associated liver fibrosis

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)

Cite this

Upregulation of cannabinoid receptor-1 and fibrotic activation of mouse hepatic stellate cells during Schistosoma J. infection : Role of NADPH oxidase. / Wang, Mi; Abais, Justine M.; Meng, Nan; Zhang, Yang; Ritter, Joseph K.; Li, Pin Lan; Tang, Wang Xian.

In: Free Radical Biology and Medicine, Vol. 71, 06.2014, p. 109-120.

Research output: Contribution to journalArticle

Wang, Mi ; Abais, Justine M. ; Meng, Nan ; Zhang, Yang ; Ritter, Joseph K. ; Li, Pin Lan ; Tang, Wang Xian. / Upregulation of cannabinoid receptor-1 and fibrotic activation of mouse hepatic stellate cells during Schistosoma J. infection : Role of NADPH oxidase. In: Free Radical Biology and Medicine. 2014 ; Vol. 71. pp. 109-120.
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abstract = "The endocannabinoid system (CS) has been implicated in the development of hepatic fibrosis such as schistosomiasis-associated liver fibrosis (SSLF). However, the mechanisms mediating the action of the CS in hepatic fibrosis are unclear. The present study hypothesized that Schistosoma J. infection upregulates cannabinoid receptor 1 (CB1) due to activation of NADPH oxidase leading to a fibrotic phenotype in hepatic stellate cells (HSCs). The SSLF model was developed by infecting mice with Schistosoma J. cercariae in the skin, and HSCs from control and infected mice were then isolated, cultured, and confirmed by analysis of HSC markers α-SMA and desmin. CB1 significantly increased in HSCs isolated from mice with SSLF, which was accompanied by a greater expression of fibrotic markers α-SMA, collagen I, and TIMP-1. CB1 upregulation and enhanced fibrotic changes were also observed in normal HSCs treated with soluble egg antigen (SEA) from Schistosoma J. Electron spin resonance (ESR) analysis further demonstrated that superoxide (O 2•-) production was increased in infected HSCs or normal HSCs stimulated with SEA. Both Nox4 and Nox1 siRNA prevented SEA-induced upregulation of CB1, α-SMA, collagen I, and TIMP-1 by inhibition of O 2•- production, while CB1 siRNA blocked SEA-induced fibrotic changes without effect on O2•- production in these HSCs. Taken together, these data suggest that the fibrotic activation of HSCs on Schistosoma J. infection or SEA stimulation is associated with NADPH oxidase-mediated redox regulation of CB1 expression, which may be a triggering mechanism for SSLF.",
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AB - The endocannabinoid system (CS) has been implicated in the development of hepatic fibrosis such as schistosomiasis-associated liver fibrosis (SSLF). However, the mechanisms mediating the action of the CS in hepatic fibrosis are unclear. The present study hypothesized that Schistosoma J. infection upregulates cannabinoid receptor 1 (CB1) due to activation of NADPH oxidase leading to a fibrotic phenotype in hepatic stellate cells (HSCs). The SSLF model was developed by infecting mice with Schistosoma J. cercariae in the skin, and HSCs from control and infected mice were then isolated, cultured, and confirmed by analysis of HSC markers α-SMA and desmin. CB1 significantly increased in HSCs isolated from mice with SSLF, which was accompanied by a greater expression of fibrotic markers α-SMA, collagen I, and TIMP-1. CB1 upregulation and enhanced fibrotic changes were also observed in normal HSCs treated with soluble egg antigen (SEA) from Schistosoma J. Electron spin resonance (ESR) analysis further demonstrated that superoxide (O 2•-) production was increased in infected HSCs or normal HSCs stimulated with SEA. Both Nox4 and Nox1 siRNA prevented SEA-induced upregulation of CB1, α-SMA, collagen I, and TIMP-1 by inhibition of O 2•- production, while CB1 siRNA blocked SEA-induced fibrotic changes without effect on O2•- production in these HSCs. Taken together, these data suggest that the fibrotic activation of HSCs on Schistosoma J. infection or SEA stimulation is associated with NADPH oxidase-mediated redox regulation of CB1 expression, which may be a triggering mechanism for SSLF.

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