Uptake of technetium-99m-tetrofosmin, technetium-99m-MIBI and thallium- 201 in tumor cell lines

Ali Syed Arbab, Kiyoshi Koizumi, Keiji Toyama, Tsutomu Araki

Research output: Contribution to journalArticle

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Abstract

We investigated the kinetics, cellular uptake and intracellular distribution of 99mTc-tetrofosmin in tumor cell lines and compared them with those of 99mTc-MIBI and 201Tl. Methods: At specific intervals after incubation with radiotracers, cellular uptake was determined. Cells were also treated with nigericin, carbonyl cyanide m-chlorophenylhydrazone (CCCP) and ouabain to determine their effects on the uptake of the tracers. Results: Each tracer showed similar uptake kinetics in both cell lines, and a steady-state was maintained for at least 4 hr. Nigericin stimulated the uptake of both 99mTc-tetrofosmin and 99mTc-MIBI in HBL-2 cells, although it inhibited their uptake in SW-13 cells. Nigericin also inhibited 90% of 201Tl uptake in both cell lines. Addition of CCCP caused 73%-97% release of accumulated 99mTc-MIBI from both cell lines with or without nigericin pretreatment, indicating that most of the accumulated 99mTc- MIBI was related to mitochondria. The effect of CCCP on accumulated 99mTc- tatrofosmin was less marked than that on 99mTc-MIBI in both cell lines, indicating that only a part of accumulated 99mTc-tetrofosmin, was related to mitochondria. Ouabain preincubation inhibited 74%-77% and 51%-53% of 201Tl uptake in HBL-2 and SW-13 cells, respectively, as well as inhibited 22%-31% uptake of 99mTc-tetrofosmin in both HBL-2 and SW-13 cells. Uptake by the dead ceils of either cell line was negligible for each tracer. Conclusion: Technetium-99m-tetrofosmin uptake depends on both cell membrane and mitochondrial potentials. Only a small fraction of 99mTc-tetrofosmin accumulates inside the mitochondria, while most 99mTc-MIBI accumulates inside the mitochondria. Thallium-201 uptake is partly independent of the Na+, K+ pump.

Original languageEnglish (US)
Pages (from-to)1551-1556
Number of pages6
JournalJournal of Nuclear Medicine
Volume37
Issue number9
StatePublished - Sep 1 1996
Externally publishedYes

Fingerprint

Thallium
Technetium
Tumor Cell Line
Nigericin
Mitochondria
Cell Line
Ouabain
Membrane Potentials
technetium Tc 99m 1,2-bis(bis(2-ethoxyethyl)phosphino)ethane
Cell Membrane
mesoxalonitrile
carbonyl 3-chlorophenylhydrazone

Keywords

  • mitochondrial and cell membrane potentials
  • technetium-99m- MIBI
  • technetium-99m-tetrofosmin
  • thallium-201
  • tumor cell lines

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Uptake of technetium-99m-tetrofosmin, technetium-99m-MIBI and thallium- 201 in tumor cell lines. / Arbab, Ali Syed; Koizumi, Kiyoshi; Toyama, Keiji; Araki, Tsutomu.

In: Journal of Nuclear Medicine, Vol. 37, No. 9, 01.09.1996, p. 1551-1556.

Research output: Contribution to journalArticle

Arbab, Ali Syed ; Koizumi, Kiyoshi ; Toyama, Keiji ; Araki, Tsutomu. / Uptake of technetium-99m-tetrofosmin, technetium-99m-MIBI and thallium- 201 in tumor cell lines. In: Journal of Nuclear Medicine. 1996 ; Vol. 37, No. 9. pp. 1551-1556.
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abstract = "We investigated the kinetics, cellular uptake and intracellular distribution of 99mTc-tetrofosmin in tumor cell lines and compared them with those of 99mTc-MIBI and 201Tl. Methods: At specific intervals after incubation with radiotracers, cellular uptake was determined. Cells were also treated with nigericin, carbonyl cyanide m-chlorophenylhydrazone (CCCP) and ouabain to determine their effects on the uptake of the tracers. Results: Each tracer showed similar uptake kinetics in both cell lines, and a steady-state was maintained for at least 4 hr. Nigericin stimulated the uptake of both 99mTc-tetrofosmin and 99mTc-MIBI in HBL-2 cells, although it inhibited their uptake in SW-13 cells. Nigericin also inhibited 90{\%} of 201Tl uptake in both cell lines. Addition of CCCP caused 73{\%}-97{\%} release of accumulated 99mTc-MIBI from both cell lines with or without nigericin pretreatment, indicating that most of the accumulated 99mTc- MIBI was related to mitochondria. The effect of CCCP on accumulated 99mTc- tatrofosmin was less marked than that on 99mTc-MIBI in both cell lines, indicating that only a part of accumulated 99mTc-tetrofosmin, was related to mitochondria. Ouabain preincubation inhibited 74{\%}-77{\%} and 51{\%}-53{\%} of 201Tl uptake in HBL-2 and SW-13 cells, respectively, as well as inhibited 22{\%}-31{\%} uptake of 99mTc-tetrofosmin in both HBL-2 and SW-13 cells. Uptake by the dead ceils of either cell line was negligible for each tracer. Conclusion: Technetium-99m-tetrofosmin uptake depends on both cell membrane and mitochondrial potentials. Only a small fraction of 99mTc-tetrofosmin accumulates inside the mitochondria, while most 99mTc-MIBI accumulates inside the mitochondria. Thallium-201 uptake is partly independent of the Na+, K+ pump.",
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T1 - Uptake of technetium-99m-tetrofosmin, technetium-99m-MIBI and thallium- 201 in tumor cell lines

AU - Arbab, Ali Syed

AU - Koizumi, Kiyoshi

AU - Toyama, Keiji

AU - Araki, Tsutomu

PY - 1996/9/1

Y1 - 1996/9/1

N2 - We investigated the kinetics, cellular uptake and intracellular distribution of 99mTc-tetrofosmin in tumor cell lines and compared them with those of 99mTc-MIBI and 201Tl. Methods: At specific intervals after incubation with radiotracers, cellular uptake was determined. Cells were also treated with nigericin, carbonyl cyanide m-chlorophenylhydrazone (CCCP) and ouabain to determine their effects on the uptake of the tracers. Results: Each tracer showed similar uptake kinetics in both cell lines, and a steady-state was maintained for at least 4 hr. Nigericin stimulated the uptake of both 99mTc-tetrofosmin and 99mTc-MIBI in HBL-2 cells, although it inhibited their uptake in SW-13 cells. Nigericin also inhibited 90% of 201Tl uptake in both cell lines. Addition of CCCP caused 73%-97% release of accumulated 99mTc-MIBI from both cell lines with or without nigericin pretreatment, indicating that most of the accumulated 99mTc- MIBI was related to mitochondria. The effect of CCCP on accumulated 99mTc- tatrofosmin was less marked than that on 99mTc-MIBI in both cell lines, indicating that only a part of accumulated 99mTc-tetrofosmin, was related to mitochondria. Ouabain preincubation inhibited 74%-77% and 51%-53% of 201Tl uptake in HBL-2 and SW-13 cells, respectively, as well as inhibited 22%-31% uptake of 99mTc-tetrofosmin in both HBL-2 and SW-13 cells. Uptake by the dead ceils of either cell line was negligible for each tracer. Conclusion: Technetium-99m-tetrofosmin uptake depends on both cell membrane and mitochondrial potentials. Only a small fraction of 99mTc-tetrofosmin accumulates inside the mitochondria, while most 99mTc-MIBI accumulates inside the mitochondria. Thallium-201 uptake is partly independent of the Na+, K+ pump.

AB - We investigated the kinetics, cellular uptake and intracellular distribution of 99mTc-tetrofosmin in tumor cell lines and compared them with those of 99mTc-MIBI and 201Tl. Methods: At specific intervals after incubation with radiotracers, cellular uptake was determined. Cells were also treated with nigericin, carbonyl cyanide m-chlorophenylhydrazone (CCCP) and ouabain to determine their effects on the uptake of the tracers. Results: Each tracer showed similar uptake kinetics in both cell lines, and a steady-state was maintained for at least 4 hr. Nigericin stimulated the uptake of both 99mTc-tetrofosmin and 99mTc-MIBI in HBL-2 cells, although it inhibited their uptake in SW-13 cells. Nigericin also inhibited 90% of 201Tl uptake in both cell lines. Addition of CCCP caused 73%-97% release of accumulated 99mTc-MIBI from both cell lines with or without nigericin pretreatment, indicating that most of the accumulated 99mTc- MIBI was related to mitochondria. The effect of CCCP on accumulated 99mTc- tatrofosmin was less marked than that on 99mTc-MIBI in both cell lines, indicating that only a part of accumulated 99mTc-tetrofosmin, was related to mitochondria. Ouabain preincubation inhibited 74%-77% and 51%-53% of 201Tl uptake in HBL-2 and SW-13 cells, respectively, as well as inhibited 22%-31% uptake of 99mTc-tetrofosmin in both HBL-2 and SW-13 cells. Uptake by the dead ceils of either cell line was negligible for each tracer. Conclusion: Technetium-99m-tetrofosmin uptake depends on both cell membrane and mitochondrial potentials. Only a small fraction of 99mTc-tetrofosmin accumulates inside the mitochondria, while most 99mTc-MIBI accumulates inside the mitochondria. Thallium-201 uptake is partly independent of the Na+, K+ pump.

KW - mitochondrial and cell membrane potentials

KW - technetium-99m- MIBI

KW - technetium-99m-tetrofosmin

KW - thallium-201

KW - tumor cell lines

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