Uracils at nucleotide position 9-11 are required for the rapid turnover of miR-29 family

Zhuo Zhang, Jun Zou, Guo Kun Wang, Jun Tao Zhang, Shuang Huang, Yong Wen Qin, Qing Jing

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

MicroRNAs are endogenous small RNA molecules that regulate gene expression. Although the biogenesis of microRNAs and their regulation have been thoroughly elucidated, the degradation of microRNAs has not been fully understood. Here by using the pulse-chase approach, we performed the direct measurement of microRNA lifespan. Five representative microRNAs demonstrated a general feature of relatively long lifespan. However, the decay dynamic varies considerably between these individual microRNAs. Mutation analysis of miR-29b sequence revealed that uracils at nucleotide position 9-11 are required for its rapid decay, in that both specific nucleotides and their position are critical. The effect of uracil-rich element on miR-29b decay dynamic occurs in duplex but not in single strand RNA. Moreover, analysis of published data on microRNA expression profile during development reveals that a substantial subset of microRNAs with the uracil-rich sequence tends to be down-regulated compared to those without the sequence. Among them, Northern blotting shows that miR-29c and fruit fly bantam possess a relatively rapid turnover rate. The effect of uracil-rich sequence on microRNA turnover depends on the sequence context. The present work indicates that microRNAs contain sequence information in the middle region besides the sequence element at both ends.

Original languageEnglish (US)
Pages (from-to)4387-4395
Number of pages9
JournalNucleic Acids Research
Volume39
Issue number10
DOIs
StatePublished - May 1 2011

Fingerprint

Uracil Nucleotides
MicroRNAs
Uracil
RNA
Diptera
Northern Blotting
Fruit
Nucleotides

ASJC Scopus subject areas

  • Genetics

Cite this

Zhang, Z., Zou, J., Wang, G. K., Zhang, J. T., Huang, S., Qin, Y. W., & Jing, Q. (2011). Uracils at nucleotide position 9-11 are required for the rapid turnover of miR-29 family. Nucleic Acids Research, 39(10), 4387-4395. https://doi.org/10.1093/nar/gkr020

Uracils at nucleotide position 9-11 are required for the rapid turnover of miR-29 family. / Zhang, Zhuo; Zou, Jun; Wang, Guo Kun; Zhang, Jun Tao; Huang, Shuang; Qin, Yong Wen; Jing, Qing.

In: Nucleic Acids Research, Vol. 39, No. 10, 01.05.2011, p. 4387-4395.

Research output: Contribution to journalArticle

Zhang, Z, Zou, J, Wang, GK, Zhang, JT, Huang, S, Qin, YW & Jing, Q 2011, 'Uracils at nucleotide position 9-11 are required for the rapid turnover of miR-29 family', Nucleic Acids Research, vol. 39, no. 10, pp. 4387-4395. https://doi.org/10.1093/nar/gkr020
Zhang, Zhuo ; Zou, Jun ; Wang, Guo Kun ; Zhang, Jun Tao ; Huang, Shuang ; Qin, Yong Wen ; Jing, Qing. / Uracils at nucleotide position 9-11 are required for the rapid turnover of miR-29 family. In: Nucleic Acids Research. 2011 ; Vol. 39, No. 10. pp. 4387-4395.
@article{a65a2e9e2b3e4b2993a71c4b40a1d9b2,
title = "Uracils at nucleotide position 9-11 are required for the rapid turnover of miR-29 family",
abstract = "MicroRNAs are endogenous small RNA molecules that regulate gene expression. Although the biogenesis of microRNAs and their regulation have been thoroughly elucidated, the degradation of microRNAs has not been fully understood. Here by using the pulse-chase approach, we performed the direct measurement of microRNA lifespan. Five representative microRNAs demonstrated a general feature of relatively long lifespan. However, the decay dynamic varies considerably between these individual microRNAs. Mutation analysis of miR-29b sequence revealed that uracils at nucleotide position 9-11 are required for its rapid decay, in that both specific nucleotides and their position are critical. The effect of uracil-rich element on miR-29b decay dynamic occurs in duplex but not in single strand RNA. Moreover, analysis of published data on microRNA expression profile during development reveals that a substantial subset of microRNAs with the uracil-rich sequence tends to be down-regulated compared to those without the sequence. Among them, Northern blotting shows that miR-29c and fruit fly bantam possess a relatively rapid turnover rate. The effect of uracil-rich sequence on microRNA turnover depends on the sequence context. The present work indicates that microRNAs contain sequence information in the middle region besides the sequence element at both ends.",
author = "Zhuo Zhang and Jun Zou and Wang, {Guo Kun} and Zhang, {Jun Tao} and Shuang Huang and Qin, {Yong Wen} and Qing Jing",
year = "2011",
month = "5",
day = "1",
doi = "10.1093/nar/gkr020",
language = "English (US)",
volume = "39",
pages = "4387--4395",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "10",

}

TY - JOUR

T1 - Uracils at nucleotide position 9-11 are required for the rapid turnover of miR-29 family

AU - Zhang, Zhuo

AU - Zou, Jun

AU - Wang, Guo Kun

AU - Zhang, Jun Tao

AU - Huang, Shuang

AU - Qin, Yong Wen

AU - Jing, Qing

PY - 2011/5/1

Y1 - 2011/5/1

N2 - MicroRNAs are endogenous small RNA molecules that regulate gene expression. Although the biogenesis of microRNAs and their regulation have been thoroughly elucidated, the degradation of microRNAs has not been fully understood. Here by using the pulse-chase approach, we performed the direct measurement of microRNA lifespan. Five representative microRNAs demonstrated a general feature of relatively long lifespan. However, the decay dynamic varies considerably between these individual microRNAs. Mutation analysis of miR-29b sequence revealed that uracils at nucleotide position 9-11 are required for its rapid decay, in that both specific nucleotides and their position are critical. The effect of uracil-rich element on miR-29b decay dynamic occurs in duplex but not in single strand RNA. Moreover, analysis of published data on microRNA expression profile during development reveals that a substantial subset of microRNAs with the uracil-rich sequence tends to be down-regulated compared to those without the sequence. Among them, Northern blotting shows that miR-29c and fruit fly bantam possess a relatively rapid turnover rate. The effect of uracil-rich sequence on microRNA turnover depends on the sequence context. The present work indicates that microRNAs contain sequence information in the middle region besides the sequence element at both ends.

AB - MicroRNAs are endogenous small RNA molecules that regulate gene expression. Although the biogenesis of microRNAs and their regulation have been thoroughly elucidated, the degradation of microRNAs has not been fully understood. Here by using the pulse-chase approach, we performed the direct measurement of microRNA lifespan. Five representative microRNAs demonstrated a general feature of relatively long lifespan. However, the decay dynamic varies considerably between these individual microRNAs. Mutation analysis of miR-29b sequence revealed that uracils at nucleotide position 9-11 are required for its rapid decay, in that both specific nucleotides and their position are critical. The effect of uracil-rich element on miR-29b decay dynamic occurs in duplex but not in single strand RNA. Moreover, analysis of published data on microRNA expression profile during development reveals that a substantial subset of microRNAs with the uracil-rich sequence tends to be down-regulated compared to those without the sequence. Among them, Northern blotting shows that miR-29c and fruit fly bantam possess a relatively rapid turnover rate. The effect of uracil-rich sequence on microRNA turnover depends on the sequence context. The present work indicates that microRNAs contain sequence information in the middle region besides the sequence element at both ends.

UR - http://www.scopus.com/inward/record.url?scp=79961187973&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79961187973&partnerID=8YFLogxK

U2 - 10.1093/nar/gkr020

DO - 10.1093/nar/gkr020

M3 - Article

VL - 39

SP - 4387

EP - 4395

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 10

ER -