Use of a specific MMP-inhibitor (galardin) for preservation of hybrid layer

Lorenzo Breschi, Patrizia Martin, Annalisa Mazzoni, Fernando Nato, Marcela Carrilho, Leo Tjäderhane, Erika Visintini, Milena Cadenaro, Franklin Chi Meng Tay, Elettra De Stefano Dorigo, David Henry Pashley

Research output: Contribution to journalArticle

120 Citations (Scopus)

Abstract

Objective: Dentinal MMPs have been claimed to contribute to the auto-degradation of collagen fibrils within incompletely resin-infiltrated hybrid layers and their inhibition may, therefore, slow the degradation of hybrid layer. This study aimed to determine the contribution of a synthetic MMPs inhibitor (galardin) to the proteolytic activity of dentinal MMPs and to the morphological and mechanical features of hybrid layers after aging. Methods: Dentin powder obtained from human molars was treated with galardin or chlorhexidine digluconate and zymographically analyzed. Microtensile bond strength was also evaluated in extracted human teeth. Exposed dentin was etched with 35% phosphoric acid and specimens were assigned to (1) pre-treatment with galardin as additional primer for 30 s and (2) no pre-treatment. A two-step etch-and-rinse adhesive (Adper Scotchbond 1XT, 3M ESPE) was then applied in accordance with manufacturer's instructions and resin composite build-ups were created. Specimens were immediately tested for their microtensile bond strength or stored in artificial saliva for 12 months prior to being tested. Data were evaluated by two-way ANOVA and Tukey's tests (α = 0.05). Additional specimens were prepared for interfacial nanoleakage analysis under light microscopy and TEM, quantified by two independent observers and statistically analyzed (χ2 test, α = 0.05). Results: The inhibitory effect of galardin on dentinal MMPs was confirmed by zymographic analysis, as complete inhibition of both MMP-2 and -9 was observed. The use of galardin had no effect on immediate bond strength, while it significantly decreased bond degradation after 1 year (p < 0.05). Interfacial nanoleakage expression after aging revealed reduced silver deposits in galardin-treated specimens compared to controls (p < 0.05). Conclusions: This study confirmed that the proteolytic activity of dentinal MMPs was inhibited by the use of galardin in a therapeutic primer. Galardin also partially preserved the mechanical integrity of the hybrid layer created by a two-step etch-and-rinse adhesive after artificial aging.

Original languageEnglish (US)
Pages (from-to)571-578
Number of pages8
JournalDental Materials
Volume26
Issue number6
DOIs
StatePublished - Jun 1 2010

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Matrix Metalloproteinase Inhibitors
Aging of materials
Degradation
Matrix Metalloproteinases
Adhesives
Silver deposits
Resins
Phosphoric acid
Analysis of variance (ANOVA)
Collagen
X ray powder diffraction
Optical microscopy
Dentin
Transmission electron microscopy
Composite materials
Artificial Saliva
N-(2(R)-2-(hydroxamidocarbonylmethyl)-4-methylpentanoyl)-L-tryptophan methylamide
Composite Resins
Silver
Powders

Keywords

  • Aging
  • Dental bonding systems
  • Dentin
  • Galardin
  • Hybrid layer

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

Cite this

Breschi, L., Martin, P., Mazzoni, A., Nato, F., Carrilho, M., Tjäderhane, L., ... Pashley, D. H. (2010). Use of a specific MMP-inhibitor (galardin) for preservation of hybrid layer. Dental Materials, 26(6), 571-578. https://doi.org/10.1016/j.dental.2010.02.007

Use of a specific MMP-inhibitor (galardin) for preservation of hybrid layer. / Breschi, Lorenzo; Martin, Patrizia; Mazzoni, Annalisa; Nato, Fernando; Carrilho, Marcela; Tjäderhane, Leo; Visintini, Erika; Cadenaro, Milena; Tay, Franklin Chi Meng; Dorigo, Elettra De Stefano; Pashley, David Henry.

In: Dental Materials, Vol. 26, No. 6, 01.06.2010, p. 571-578.

Research output: Contribution to journalArticle

Breschi, L, Martin, P, Mazzoni, A, Nato, F, Carrilho, M, Tjäderhane, L, Visintini, E, Cadenaro, M, Tay, FCM, Dorigo, EDS & Pashley, DH 2010, 'Use of a specific MMP-inhibitor (galardin) for preservation of hybrid layer', Dental Materials, vol. 26, no. 6, pp. 571-578. https://doi.org/10.1016/j.dental.2010.02.007
Breschi L, Martin P, Mazzoni A, Nato F, Carrilho M, Tjäderhane L et al. Use of a specific MMP-inhibitor (galardin) for preservation of hybrid layer. Dental Materials. 2010 Jun 1;26(6):571-578. https://doi.org/10.1016/j.dental.2010.02.007
Breschi, Lorenzo ; Martin, Patrizia ; Mazzoni, Annalisa ; Nato, Fernando ; Carrilho, Marcela ; Tjäderhane, Leo ; Visintini, Erika ; Cadenaro, Milena ; Tay, Franklin Chi Meng ; Dorigo, Elettra De Stefano ; Pashley, David Henry. / Use of a specific MMP-inhibitor (galardin) for preservation of hybrid layer. In: Dental Materials. 2010 ; Vol. 26, No. 6. pp. 571-578.
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abstract = "Objective: Dentinal MMPs have been claimed to contribute to the auto-degradation of collagen fibrils within incompletely resin-infiltrated hybrid layers and their inhibition may, therefore, slow the degradation of hybrid layer. This study aimed to determine the contribution of a synthetic MMPs inhibitor (galardin) to the proteolytic activity of dentinal MMPs and to the morphological and mechanical features of hybrid layers after aging. Methods: Dentin powder obtained from human molars was treated with galardin or chlorhexidine digluconate and zymographically analyzed. Microtensile bond strength was also evaluated in extracted human teeth. Exposed dentin was etched with 35{\%} phosphoric acid and specimens were assigned to (1) pre-treatment with galardin as additional primer for 30 s and (2) no pre-treatment. A two-step etch-and-rinse adhesive (Adper Scotchbond 1XT, 3M ESPE) was then applied in accordance with manufacturer's instructions and resin composite build-ups were created. Specimens were immediately tested for their microtensile bond strength or stored in artificial saliva for 12 months prior to being tested. Data were evaluated by two-way ANOVA and Tukey's tests (α = 0.05). Additional specimens were prepared for interfacial nanoleakage analysis under light microscopy and TEM, quantified by two independent observers and statistically analyzed (χ2 test, α = 0.05). Results: The inhibitory effect of galardin on dentinal MMPs was confirmed by zymographic analysis, as complete inhibition of both MMP-2 and -9 was observed. The use of galardin had no effect on immediate bond strength, while it significantly decreased bond degradation after 1 year (p < 0.05). Interfacial nanoleakage expression after aging revealed reduced silver deposits in galardin-treated specimens compared to controls (p < 0.05). Conclusions: This study confirmed that the proteolytic activity of dentinal MMPs was inhibited by the use of galardin in a therapeutic primer. Galardin also partially preserved the mechanical integrity of the hybrid layer created by a two-step etch-and-rinse adhesive after artificial aging.",
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AU - Carrilho, Marcela

AU - Tjäderhane, Leo

AU - Visintini, Erika

AU - Cadenaro, Milena

AU - Tay, Franklin Chi Meng

AU - Dorigo, Elettra De Stefano

AU - Pashley, David Henry

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N2 - Objective: Dentinal MMPs have been claimed to contribute to the auto-degradation of collagen fibrils within incompletely resin-infiltrated hybrid layers and their inhibition may, therefore, slow the degradation of hybrid layer. This study aimed to determine the contribution of a synthetic MMPs inhibitor (galardin) to the proteolytic activity of dentinal MMPs and to the morphological and mechanical features of hybrid layers after aging. Methods: Dentin powder obtained from human molars was treated with galardin or chlorhexidine digluconate and zymographically analyzed. Microtensile bond strength was also evaluated in extracted human teeth. Exposed dentin was etched with 35% phosphoric acid and specimens were assigned to (1) pre-treatment with galardin as additional primer for 30 s and (2) no pre-treatment. A two-step etch-and-rinse adhesive (Adper Scotchbond 1XT, 3M ESPE) was then applied in accordance with manufacturer's instructions and resin composite build-ups were created. Specimens were immediately tested for their microtensile bond strength or stored in artificial saliva for 12 months prior to being tested. Data were evaluated by two-way ANOVA and Tukey's tests (α = 0.05). Additional specimens were prepared for interfacial nanoleakage analysis under light microscopy and TEM, quantified by two independent observers and statistically analyzed (χ2 test, α = 0.05). Results: The inhibitory effect of galardin on dentinal MMPs was confirmed by zymographic analysis, as complete inhibition of both MMP-2 and -9 was observed. The use of galardin had no effect on immediate bond strength, while it significantly decreased bond degradation after 1 year (p < 0.05). Interfacial nanoleakage expression after aging revealed reduced silver deposits in galardin-treated specimens compared to controls (p < 0.05). Conclusions: This study confirmed that the proteolytic activity of dentinal MMPs was inhibited by the use of galardin in a therapeutic primer. Galardin also partially preserved the mechanical integrity of the hybrid layer created by a two-step etch-and-rinse adhesive after artificial aging.

AB - Objective: Dentinal MMPs have been claimed to contribute to the auto-degradation of collagen fibrils within incompletely resin-infiltrated hybrid layers and their inhibition may, therefore, slow the degradation of hybrid layer. This study aimed to determine the contribution of a synthetic MMPs inhibitor (galardin) to the proteolytic activity of dentinal MMPs and to the morphological and mechanical features of hybrid layers after aging. Methods: Dentin powder obtained from human molars was treated with galardin or chlorhexidine digluconate and zymographically analyzed. Microtensile bond strength was also evaluated in extracted human teeth. Exposed dentin was etched with 35% phosphoric acid and specimens were assigned to (1) pre-treatment with galardin as additional primer for 30 s and (2) no pre-treatment. A two-step etch-and-rinse adhesive (Adper Scotchbond 1XT, 3M ESPE) was then applied in accordance with manufacturer's instructions and resin composite build-ups were created. Specimens were immediately tested for their microtensile bond strength or stored in artificial saliva for 12 months prior to being tested. Data were evaluated by two-way ANOVA and Tukey's tests (α = 0.05). Additional specimens were prepared for interfacial nanoleakage analysis under light microscopy and TEM, quantified by two independent observers and statistically analyzed (χ2 test, α = 0.05). Results: The inhibitory effect of galardin on dentinal MMPs was confirmed by zymographic analysis, as complete inhibition of both MMP-2 and -9 was observed. The use of galardin had no effect on immediate bond strength, while it significantly decreased bond degradation after 1 year (p < 0.05). Interfacial nanoleakage expression after aging revealed reduced silver deposits in galardin-treated specimens compared to controls (p < 0.05). Conclusions: This study confirmed that the proteolytic activity of dentinal MMPs was inhibited by the use of galardin in a therapeutic primer. Galardin also partially preserved the mechanical integrity of the hybrid layer created by a two-step etch-and-rinse adhesive after artificial aging.

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