Use of electrophoretic karyotyping in the evaluation of Candida infections in a neonatal intensive-care unit

Jose Antonio Vazquez, Dina Boikov, Sergei G. Boikov, Adnan S. Dajani

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

OBJECTIVE: To evaluate a possible common-source outbreak of Candida infections in the neonatal intensive-care unit. Systemic Candida infections increased from 6 to 11 cases (0.71 to 1.34 per 1,000 patient-days). In addition, Candida parapsilosis infections increased from 1 in 1992 to 10 in 1993. DESIGN AND SETTING: Tertiary-care, teaching, pediatric institution with a 40-bed neonatal intensive-care unit (NICU). Clinical characteristics, associated conditions, and antimicrobial therapy were obtained from the medical records of all NICU patients with positive blood cultures for Candida during 1992 and 1993. Nineteen Candida isolates from 15 infants were studied retrospectively using contour-clamped homogeneous electric-field (CHEF) electrophoresis. RESULTS: CHEF revealed eight karyotypes of C parapsilosis. Five isolates recovered from four patients shared one karyotype. The remaining isolates from seven infants all had distinctly different karyotypes. CONCLUSIONS: The evidence was insufficient to implicate a single source of infection, even though four patients in the same unit had identical strain types. However, identical strains of C parapsilosis were associated geographically, suggesting that nosocomial acquisition of C parapsilosis through indirect patient contact in the NICU was possible. The CHEF technique yields unique patterns that may be used to delineate clinical isolates and to study the molecular epidemiology of candidal infections.

Original languageEnglish (US)
Pages (from-to)32-37
Number of pages6
JournalInfection Control and Hospital Epidemiology
Volume18
Issue number1
DOIs
StatePublished - Jan 1 1997

Fingerprint

Karyotyping
Neonatal Intensive Care Units
Candida
Karyotype
Infection
Molecular Epidemiology
Tertiary Healthcare
Medical Records
Disease Outbreaks
Electrophoresis
Teaching
Pediatrics

ASJC Scopus subject areas

  • Epidemiology
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Use of electrophoretic karyotyping in the evaluation of Candida infections in a neonatal intensive-care unit. / Vazquez, Jose Antonio; Boikov, Dina; Boikov, Sergei G.; Dajani, Adnan S.

In: Infection Control and Hospital Epidemiology, Vol. 18, No. 1, 01.01.1997, p. 32-37.

Research output: Contribution to journalArticle

@article{a148631fbdfe43bfa86323d80c56db14,
title = "Use of electrophoretic karyotyping in the evaluation of Candida infections in a neonatal intensive-care unit",
abstract = "OBJECTIVE: To evaluate a possible common-source outbreak of Candida infections in the neonatal intensive-care unit. Systemic Candida infections increased from 6 to 11 cases (0.71 to 1.34 per 1,000 patient-days). In addition, Candida parapsilosis infections increased from 1 in 1992 to 10 in 1993. DESIGN AND SETTING: Tertiary-care, teaching, pediatric institution with a 40-bed neonatal intensive-care unit (NICU). Clinical characteristics, associated conditions, and antimicrobial therapy were obtained from the medical records of all NICU patients with positive blood cultures for Candida during 1992 and 1993. Nineteen Candida isolates from 15 infants were studied retrospectively using contour-clamped homogeneous electric-field (CHEF) electrophoresis. RESULTS: CHEF revealed eight karyotypes of C parapsilosis. Five isolates recovered from four patients shared one karyotype. The remaining isolates from seven infants all had distinctly different karyotypes. CONCLUSIONS: The evidence was insufficient to implicate a single source of infection, even though four patients in the same unit had identical strain types. However, identical strains of C parapsilosis were associated geographically, suggesting that nosocomial acquisition of C parapsilosis through indirect patient contact in the NICU was possible. The CHEF technique yields unique patterns that may be used to delineate clinical isolates and to study the molecular epidemiology of candidal infections.",
author = "Vazquez, {Jose Antonio} and Dina Boikov and Boikov, {Sergei G.} and Dajani, {Adnan S.}",
year = "1997",
month = "1",
day = "1",
doi = "10.2307/30141961",
language = "English (US)",
volume = "18",
pages = "32--37",
journal = "Infection Control and Hospital Epidemiology",
issn = "0899-823X",
publisher = "University of Chicago Press",
number = "1",

}

TY - JOUR

T1 - Use of electrophoretic karyotyping in the evaluation of Candida infections in a neonatal intensive-care unit

AU - Vazquez, Jose Antonio

AU - Boikov, Dina

AU - Boikov, Sergei G.

AU - Dajani, Adnan S.

PY - 1997/1/1

Y1 - 1997/1/1

N2 - OBJECTIVE: To evaluate a possible common-source outbreak of Candida infections in the neonatal intensive-care unit. Systemic Candida infections increased from 6 to 11 cases (0.71 to 1.34 per 1,000 patient-days). In addition, Candida parapsilosis infections increased from 1 in 1992 to 10 in 1993. DESIGN AND SETTING: Tertiary-care, teaching, pediatric institution with a 40-bed neonatal intensive-care unit (NICU). Clinical characteristics, associated conditions, and antimicrobial therapy were obtained from the medical records of all NICU patients with positive blood cultures for Candida during 1992 and 1993. Nineteen Candida isolates from 15 infants were studied retrospectively using contour-clamped homogeneous electric-field (CHEF) electrophoresis. RESULTS: CHEF revealed eight karyotypes of C parapsilosis. Five isolates recovered from four patients shared one karyotype. The remaining isolates from seven infants all had distinctly different karyotypes. CONCLUSIONS: The evidence was insufficient to implicate a single source of infection, even though four patients in the same unit had identical strain types. However, identical strains of C parapsilosis were associated geographically, suggesting that nosocomial acquisition of C parapsilosis through indirect patient contact in the NICU was possible. The CHEF technique yields unique patterns that may be used to delineate clinical isolates and to study the molecular epidemiology of candidal infections.

AB - OBJECTIVE: To evaluate a possible common-source outbreak of Candida infections in the neonatal intensive-care unit. Systemic Candida infections increased from 6 to 11 cases (0.71 to 1.34 per 1,000 patient-days). In addition, Candida parapsilosis infections increased from 1 in 1992 to 10 in 1993. DESIGN AND SETTING: Tertiary-care, teaching, pediatric institution with a 40-bed neonatal intensive-care unit (NICU). Clinical characteristics, associated conditions, and antimicrobial therapy were obtained from the medical records of all NICU patients with positive blood cultures for Candida during 1992 and 1993. Nineteen Candida isolates from 15 infants were studied retrospectively using contour-clamped homogeneous electric-field (CHEF) electrophoresis. RESULTS: CHEF revealed eight karyotypes of C parapsilosis. Five isolates recovered from four patients shared one karyotype. The remaining isolates from seven infants all had distinctly different karyotypes. CONCLUSIONS: The evidence was insufficient to implicate a single source of infection, even though four patients in the same unit had identical strain types. However, identical strains of C parapsilosis were associated geographically, suggesting that nosocomial acquisition of C parapsilosis through indirect patient contact in the NICU was possible. The CHEF technique yields unique patterns that may be used to delineate clinical isolates and to study the molecular epidemiology of candidal infections.

UR - http://www.scopus.com/inward/record.url?scp=0030635977&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030635977&partnerID=8YFLogxK

U2 - 10.2307/30141961

DO - 10.2307/30141961

M3 - Article

C2 - 9013244

AN - SCOPUS:0030635977

VL - 18

SP - 32

EP - 37

JO - Infection Control and Hospital Epidemiology

JF - Infection Control and Hospital Epidemiology

SN - 0899-823X

IS - 1

ER -