Abstract
The Y alphoid deoxyribonucleic acid probe Y97 has proved to be specific for the human Y centromere and to define a Y-specific 5.5 kb Eco RI fragment. Three experiments were designed to evaluate the sensitivity and the specificity of this Y alphoid probe Y97. In the first experiment the centromeric Y-specific 5.5 kb Eco RI fragment was clearly seen in the mixture of 0.050 μg of male DNA with 4.950 μg of female DNA (1%). In the second experiment the same dilutional study was applied to the Yq11-related probe 4B-2 for comparison purpose. In the third experiment, hybridization with the Y97 probe was performed on 20 subjects with mosaic cell lines containing a cytogenetically identifiable Y (n = 10) and a cytogenetically unidentifiable minute (n = 10) fragment. Nineteen of the 20 study subjects demonstrated the Y-specific 5.5 kb Eco RI hybridization band with the centromeric Y97 probe. These experiments demonstrated the utility of the Y97 probe to consistently identify cytogenetically altered Y chromosome fragments and confirm the mapping of the alphoid repeat sequences to the centromeric region of the Y chromosome.
Original language | English (US) |
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Pages (from-to) | 1553-1557 |
Number of pages | 5 |
Journal | American journal of obstetrics and gynecology |
Volume | 159 |
Issue number | 6 |
DOIs | |
State | Published - Dec 1988 |
Keywords
- 4B-2
- Y chromosome
- Y97
- deoxyribonucleic acid probes
ASJC Scopus subject areas
- Obstetrics and Gynecology